scholarly journals Changes of Membrane Proteins and Their Relation to Deoxyribonucleic Acid Synthesis and Cell Division of Escherichia coli

1970 ◽  
Vol 245 (21) ◽  
pp. 5813-5819 ◽  
Author(s):  
Masayori Inouye ◽  
Arthur B. Pardee
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Membrane Proteins ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Deoxyribonucleic Acid Synthesis

Analysis of cell division in rapidly growing synchronous cultures of Escherichia coli recovering from an inhibition of deoxyribonucleic acid synthesis

1973 ◽  
Vol 19 (9) ◽  
pp. 1111-1114 ◽  
Author(s):  
George G. Khachatourians ◽  
Lorraine M. McGrath
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Short Pulses ◽  
Synchronous Cultures ◽  
Deoxyribonucleic Acid Synthesis ◽  
Casamino Acids ◽  
Inhibition Of Dna Synthesis ◽  
Kinetics Of

Kinetics of cell division in cultures of Escherichia coli grown in casamino acids – glucose and recovering from short pulses of inhibition of DNA synthesis by nalidixic acid clearly demonstrates that preexisting cycles of chromosome replication continue to their ends.

Duration of Availability of Tritiated Thymidine Following Intraperitoneal Injection

1968 ◽  
Vol 3 (1) ◽  
pp. 89-93
Author(s):  
W. K. BLENKINSOPP
Keyword(s):  
Cell Division ◽  
Intraperitoneal Injection ◽  
Deoxyribonucleic Acid ◽  
Direct Evidence ◽  
Tritiated Thymidine ◽  
Indirect Evidence ◽  
Acid Synthesis ◽  
Single Intraperitoneal Injection ◽  
Deoxyribonucleic Acid Synthesis

Much indirect evidence supports the assumption that tritiated thymidine does not label cells which enter the deoxyribonucleic acid synthesis phase (S) more than 1 h after injection. Direct evidence confirming this assumption was obtained by counting labelled epithelial nuclei in mice killed 1, 4 or 6 h after a single intraperitoneal injection of [3H]thymidine; colchicine was used to prevent the increase in number of labelled nuclei which would otherwise have occurred because of cell division. The proportion of cells labelled was the same at 1 h as at 4 or 6 h after injection of [3H]thymidine. Nuclei were regarded as labelled if they were overlaid by 4 grains or more; comparison of nuclear and background labelling indicated that nuclei overlaid by 3 grains or less represented background labelling.

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