scholarly journals Effect of heat and 2-mercaptoethanol on intracytoplasmic membrane polypeptides of Rhodopseudomonas sphaeroides.

1978 ◽  
Vol 135 (2) ◽  
pp. 656-667 ◽  
Author(s):  
W D Shepherd ◽  
S Kaplan
Keyword(s):  
Rhodopseudomonas Sphaeroides ◽  
Intracytoplasmic Membrane

scholarly journals Kinetic analysis of N-acylphosphatidylserine accumulation and implications for membrane assembly in Rhodopseudomonas sphaeroides

1982 ◽  
Vol 152 (2) ◽  
pp. 607-615
Author(s):  
B D Cain ◽  
T J Donohue ◽  
S Kaplan
Keyword(s):  
Kinetic Analysis ◽  
Growth Medium ◽  
Metabolic Pathway ◽  
Fold Increase ◽  
Phospholipid Content ◽  
Membrane Biogenesis ◽  
Rhodopseudomonas Sphaeroides ◽  
Intracytoplasmic Membrane ◽  
Phospholipid Transfer ◽  
Cellular Phospholipid

The accumulation of N-acylphosphatidylserine (NAPS) in response to the inclusion of Tris in the growth medium of Rhodopseudomonas sphaeroides strain M29-5 has been examined. In the accompanying paper (Donohue et al., J. Bacteriol. 152:000--000, 1982), we show that in response to Tris, NAPS accumulated to as much as 40% of the total cellular phospholipid content. NAPS accumulation began immediately upon addition of Tris and was reflected as an abrupt 12-fold increase in the apparent rate of NAPS accumulation. We suggest that Tris altered the flow of metabolites through a preexisting and previously unknown metabolic pathway. NAPS accumulation ceased immediately upon the removal of Tris; however, accumulated NAPS remained largely metabolically stable. Importantly, under conditions in which NAPS was not accumulated, the intracytoplasmic membrane was shown to be virtually devoid of newly synthesized NAPS. The significance of this observation is discussed in terms of its physiological implications on phospholipid transfer and membrane biogenesis in R. sphaeroides.

A Freeze-Etch Study of Acinetobacter SP. Grown on a Hydrocarbon Substrate

1974 ◽  
Vol 32 ◽  
pp. 174-175
Author(s):  
C. L. Scott ◽  
W. R. Finnerty
Keyword(s):  
Membrane System ◽  
Structural Distortion ◽  
Intracytoplasmic Membrane ◽  
Gram Negative ◽  
Sectioned Material ◽  
Acinetobacter Sp

Acinetobacter sp. HO-1-N, a gram-negative hydrocarbon oxidizing bacterium previously designated Micrococcus cerificans, has been shown to sequester the hydrocarbon into intracytoplasmic pools as a result of growth on this substrate. In hydrocarbon grown cells, an intracytoplasmic membrane system was also observed along with a doubling of cellular phospholipids (Z). However, using conventional dehydration and embedding procedures in preparing thin sectioned material, the hydrocarbon is extracted from the cells. This may lead to structural distortion, consequently, the freeze-etch technique was applied to preserve the integrity of the cell.

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