scholarly journals Intracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Polypeptide insertion into growing membrane.

1978 ◽  
Vol 253 (2) ◽  
pp. 458-464 ◽  
Author(s):  
R.T. Fraley ◽  
D.R. Lueking ◽  
S. Kaplan
Keyword(s):  
Cell Populations ◽  
Rhodopseudomonas Sphaeroides ◽  
Intracytoplasmic Membrane ◽  
Membrane Synthesis ◽  
Synchronous Cell

scholarly journals Kinetic analysis of N-acylphosphatidylserine accumulation and implications for membrane assembly in Rhodopseudomonas sphaeroides

1982 ◽  
Vol 152 (2) ◽  
pp. 607-615
Author(s):  
B D Cain ◽  
T J Donohue ◽  
S Kaplan
Keyword(s):  
Kinetic Analysis ◽  
Growth Medium ◽  
Metabolic Pathway ◽  
Fold Increase ◽  
Phospholipid Content ◽  
Membrane Biogenesis ◽  
Rhodopseudomonas Sphaeroides ◽  
Intracytoplasmic Membrane ◽  
Phospholipid Transfer ◽  
Cellular Phospholipid

The accumulation of N-acylphosphatidylserine (NAPS) in response to the inclusion of Tris in the growth medium of Rhodopseudomonas sphaeroides strain M29-5 has been examined. In the accompanying paper (Donohue et al., J. Bacteriol. 152:000--000, 1982), we show that in response to Tris, NAPS accumulated to as much as 40% of the total cellular phospholipid content. NAPS accumulation began immediately upon addition of Tris and was reflected as an abrupt 12-fold increase in the apparent rate of NAPS accumulation. We suggest that Tris altered the flow of metabolites through a preexisting and previously unknown metabolic pathway. NAPS accumulation ceased immediately upon the removal of Tris; however, accumulated NAPS remained largely metabolically stable. Importantly, under conditions in which NAPS was not accumulated, the intracytoplasmic membrane was shown to be virtually devoid of newly synthesized NAPS. The significance of this observation is discussed in terms of its physiological implications on phospholipid transfer and membrane biogenesis in R. sphaeroides.

scholarly journals Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis

2021 ◽  
Vol 22 (19) ◽  
pp. 10759
Author(s):  
Anna Ligasová ◽  
Karel Koberna
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Mammalian Cells ◽  
Building Blocks ◽  
Cell Populations ◽  
Advantages And Disadvantages ◽  
Cell Synchronization ◽  
Synchronous Cell ◽  
Inhibition Of Dna Synthesis ◽  
Selection Of

Synchronous cell populations are commonly used for the analysis of various aspects of cellular metabolism at specific stages of the cell cycle. Cell synchronization at a chosen cell cycle stage is most frequently achieved by inhibition of specific metabolic pathway(s). In this respect, various protocols have been developed to synchronize cells in particular cell cycle stages. In this review, we provide an overview of the protocols for cell synchronization of mammalian cells based on the inhibition of synthesis of DNA building blocks—deoxynucleotides and/or inhibition of DNA synthesis. The mechanism of action, examples of their use, and advantages and disadvantages are described with the aim of providing a guide for the selection of suitable protocol for different studied situations.

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