scholarly journals Control of transcription of the Bacillus subtilis spoIIIG gene, which codes for the forespore-specific transcription factor sigma G.

1991 ◽  
Vol 173 (9) ◽  
pp. 2977-2984 ◽  
Author(s):  
D X Sun ◽  
R M Cabrera-Martinez ◽  
P Setlow
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Specific Transcription Factor ◽  
Sigma G

scholarly journals Genotype, Phenotype, and Protein Structure in a Regulator of Sporulation: Effects of Mutations in the spoIIAA Gene ofBacillus subtilis

1999 ◽  
Vol 181 (12) ◽  
pp. 3860-3863 ◽  
Author(s):  
Daniela Barillà ◽  
Isabelle Lucet ◽  
Anne Kuhlmann ◽  
Michael D. Yudkin
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Protein Structure ◽  
Solution Structure ◽  
Protein A ◽  
Ofbacillus Subtilis ◽  
Specific Transcription Factor ◽  
Mutant Strains

ABSTRACT SpoIIAA, a phosphorylatable protein, is essential to the regulation of ςF, the first sporulation-specific transcription factor of Bacillus subtilis. The solution structure of SpoIIAA has recently been published. Here we examine four mutant SpoIIAA proteins and correlate their properties with the phenotypes of the corresponding B. subtilis mutant strains. Two of the mutations severely disrupted the structure of the protein, a third greatly diminished the rate of its phosphorylation and abolished dephosphorylation, and the fourth left phosphorylation unaffected but reduced the rate of dephosphorylation about 10-fold.

scholarly journals Unique Degradation Signal for ClpCP in Bacillus subtilis

2003 ◽  
Vol 185 (17) ◽  
pp. 5275-5278 ◽  
Author(s):  
Qi Pan ◽  
Richard Losick
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Specific Transcription Factor ◽  
C Terminus ◽  
Antisigma Factor ◽  
Necessary And Sufficient

ABSTRACT Regulation of the cell-specific transcription factor σF in the spore-forming bacterium Bacillus subtilis involves the antisigma factor SpoIIAB. Contributing to the activation of σF is the degradation of SpoIIAB in a manner that depends on the protease ClpCP. Here we show that the three residues (LCN) located at the extreme C terminus of SpoIIAB are both necessary and sufficient for this degradation. We also report that the use of the LCN extension as a degradation signal for ClpCP is unique to SpoIIAB.

Identification of sporulation genes by genome-wide analysis of the σ E regulon of Bacillus subtilis

Microbiology ◽  
2003 ◽  
Vol 149 (10) ◽  
pp. 3023-3034 ◽  
Author(s):  
Andrea Feucht ◽  
Louise Evans ◽  
Jeff Errington
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Transcription Factors ◽  
Mother Cell ◽  
Dna Microarrays ◽  
Genome Wide Analysis ◽  
Specific Transcription Factor ◽  
Genome Wide ◽  
Sequential Activation ◽  
Sporulation Genes

Differentiation in the spore-forming bacterium Bacillus subtilis is governed by the sequential activation of five sporulation-specific transcription factors. The early mother-cell-specific transcription factor, σ E, directs the transcription of many genes that contribute to the formation of mature, dormant spores. In this study, DNA microarrays were used to identify genes belonging to the σ E regulon. In total, 171 genes were found to be under the control of σ E. Of these, 101 genes had not previously been described as being σ E dependent. Disruption of some of the previously unknown genes (ydcC, yhaL, yhbH, yjaV and yqfD) resulted in a defect in sporulation.

scholarly journals Novel spoIIE Mutation That Causes Uncompartmentalized σF Activation in Bacillus subtilis

2003 ◽  
Vol 185 (5) ◽  
pp. 1590-1598 ◽  
Author(s):  
David W. Hilbert ◽  
Patrick J. Piggot
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Mother Cell ◽  
Critical Role ◽  
Asymmetric Division ◽  
Small Subset ◽  
Gene Encoding ◽  
Specific Transcription Factor ◽  
Fold Reduction ◽  
Optimal Efficiency

ABSTRACT During sporulation, Bacillus subtilis undergoes an asymmetric division that results in two cells with different fates, the larger mother cell and the smaller forespore. The protein phosphatase SpoIIE, which is required for activation of the forespore-specific transcription factor σF, is also required for optimal efficiency and timing of asymmetric division. We performed a genetic screen for spoIIE mutants that were impaired in sporulation but not σF activity and isolated a strain with the mutation spoIIEV697A. The mutant exhibited a 10- to 40-fold reduction in sporulation and a sixfold reduction in asymmetric division compared to the parent. Transcription of the σF-dependent spoIIQ promoter was increased more than 10-fold and was no longer confined to the forespore. The excessive σF activity persisted even when asymmetric division was prevented. Disruption of spoIIGB did not restore asymmetric division to the spoIIEV697A mutant, indicating that the deficiency is not a consequence of predivisional activation of the mother cell-specific transcription factor σE. Deletion of the gene encoding σF (spoIIAC) restored asymmetric division; however, a mutation that dramatically reduced the number of promoters responsive to σF, spoIIAC561 (spoIIACV233 M), failed to do so. This result suggests that the block is due to expression of one of the small subset of σF-dependent genes expressed in this background or to unregulated interaction of σF with some other factor. Our results indicate that regulation of SpoIIE plays a critical role in coupling asymmetric division to σF activation in order to ensure proper spatial and temporal expression of forespore-specific genes.

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