scholarly journals Physiological and biochemical analyses of FlgH, a lipoprotein forming the outer membrane L ring of the flagellar basal body of Salmonella typhimurium.

1996 ◽  
Vol 178 (14) ◽  
pp. 4200-4207 ◽  
Author(s):  
G J Schoenhals ◽  
R M Macnab
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Basal Body ◽  
Biochemical Analyses ◽  
Physiological And Biochemical

​Optimization of Cultural Conditions for Maximum Production of Outer Membrane Vesicles from Salmonella Typhimurium

2021 ◽  
Author(s):  
Menguzotunuo Solo ◽  
Shantanu Tamuly ◽  
Luit Moni Barkalita ◽  
Girin Kumar Saikia ◽  
Dhruba Jyoti Kalita
Keyword(s):  
Oxidative Stress ◽  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Large Scale ◽  
Multiple Drug Resistance ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multiple Drug ◽  
Scale Production ◽  
Maximum Production

Background: The non-typhoidal Salmonella causes gastroenteritis in humans that makes its way to the food chain mainly through the animal products. The multiple drug resistance imposes one of the major hurdle in the treatment of the disease. The vaccination appears to be the most important method for prevention of the disease. Unfortunately, there is no liscenced vaccine available against non-typhoidal Salmonellae. The use of outer membrane vesicles (OMVs) of Salmonella as a vaccine candidate has attained significant centre-stage in the recent years given to its protective immunogenicity. However, the large scale production of OMVs is difficult owing to low yield per liter of culture. Methods: In the present study, we have optimized the culture conditions viz. pH, phase of growth and presence of oxidative stress for maximum production of OMVs from Salmonella Typhimurium. The OMVs were characterized based on yield based on protein concentration, lipopolysaccharide concentration and zeta size. Result: In the present study, it was found that incubation of Salmonella Typhimurium up to peak of the growth phase at pH 7 in presence of oxidative stress was found to be the most suitable condition for maximum production of OMVs.

scholarly journals Topology of the outer membrane phospholipase A of Salmonella typhimurium.

1997 ◽  
Vol 179 (11) ◽  
pp. 3443-3450 ◽  
Author(s):  
K B Merck ◽  
H de Cock ◽  
H M Verheij ◽  
J Tommassen
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Phospholipase A ◽  
Outer Membrane Phospholipase A

Characterization and sequence analysis of potential biofertilizer and biocontrol agent Bacillus subtilis strain SEM-9 from silkworm excrement

2019 ◽  
Vol 65 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Qingrong Li ◽  
Sentai Liao ◽  
Huyu Zhi ◽  
Dongxu Xing ◽  
Yang Xiao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Gene Clusters ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Fusarium Spp ◽  
Antagonistic Microorganisms ◽  
Biochemical Analyses ◽  
Physiological And Biochemical

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. The result of physiological and biochemical analyses, as well as genome sequencing, showed that SEM-9 was a strain of Bacillus subtilis. Through genome annotation and analysis, it was found that SEM-9 contained genes related to the regulation of biofilm formation, which may play an important role in colonization, and gene clusters encoding the biosynthesis of antimicrobials, such as surfactin, bacilysin, fengycin, and subtilosin-A. The production of such antifungal compounds may constitute the basis of the mode-of-action of SEM-9 against Fusarium spp. These data suggested that the SEM-9 strain has potential as both a biofertilizer and a biocontrol agent, with the potential to manage Fusarium wilt disease in crops.

Periplasmic quality control in biogenesis of outer membrane proteins

2015 ◽  
Vol 43 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Zhi Xin Lyu ◽  
Xin Sheng Zhao
Keyword(s):  
Quality Control ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Diverse Range ◽  
Multifunctional Protein ◽  
Proposed Model ◽  
Periplasmic Proteins ◽  
Basic Functions ◽  
Biochemical Analyses

The β-barrel outer membrane proteins (OMPs) are integral membrane proteins that reside in the outer membrane of Gram-negative bacteria and perform a diverse range of biological functions. Synthesized in the cytoplasm, OMPs must be transported across the inner membrane and through the periplasmic space before they are assembled in the outer membrane. In Escherichia coli, Skp, SurA and DegP are the most prominent factors identified to guide OMPs across the periplasm and to play the role of quality control. Although extensive genetic and biochemical analyses have revealed many basic functions of these periplasmic proteins, the mechanism of their collaboration in assisting the folding and insertion of OMPs is much less understood. Recently, biophysical approaches have shed light on the identification of the intricate network. In the present review, we summarize recent advances in the characterization of these key factors, with a special emphasis on the multifunctional protein DegP. In addition, we present our proposed model on the periplasmic quality control in biogenesis of OMPs.

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