scholarly journals The N-Terminal Region of the Escherichia coli WecA (Rfe) Protein, Containing Three Predicted Transmembrane Helices, Is Required for Function but Not for Membrane Insertion

2000 ◽  
Vol 182 (2) ◽  
pp. 498-503 ◽  
Author(s):  
Amal O. Amer ◽  
Miguel A. Valvano
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Terminal Region ◽  
Membrane Localization ◽  
Membrane Insertion ◽  
Transmembrane Helices

ABSTRACT The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.

Amphipathic C-terminal region of Escherichia coli NADH dehydrogenase-2 mediates membrane localization

2011 ◽  
Vol 505 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Josefina M. Villegas ◽  
Sabrina I. Volentini ◽  
María R. Rintoul ◽  
Viviana A. Rapisarda
Keyword(s):  
Escherichia Coli ◽  
Nadh Dehydrogenase ◽  
Terminal Region ◽  
Membrane Localization

scholarly journals Mutually exclusive RNA secondary structures regulate translation initiation of DinQ in Escherichia coli

RNA ◽  
2016 ◽  
Vol 22 (11) ◽  
pp. 1739-1749 ◽  
Author(s):  
Knut I. Kristiansen ◽  
Ragnhild Weel-Sneve ◽  
James A. Booth ◽  
Magnar Bjørås
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Secondary Structures ◽  
Rna Secondary Structures

scholarly journals Point Mutations in Transmembrane Helices 2 and 3 of ExbB and TolQ Affect Their Activities in Escherichia coli K-12

2004 ◽  
Vol 186 (13) ◽  
pp. 4402-4406 ◽  
Author(s):  
Volkmar Braun ◽  
Christina Herrmann
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Transmembrane Helix ◽  
Point Mutations ◽  
Transmembrane Helices ◽  
The Third ◽  
Form Part ◽  
K 12

ABSTRACT Replacement of glutamate 176, the only charged amino acid in the third transmembrane helix of ExbB, with alanine (E176A) abolished ExbB activity in all determined ExbB-dependent functions of Escherichia coli. Combination of the mutations T148A in the second transmembrane helix and T181A in the third transmembrane helix, proposed to form part of a proton pathway through ExbB, also resulted in inactive ExbB. E176 and T148 are strictly conserved in ExbB and TolQ proteins, and T181 is almost strictly conserved in ExbB, TolQ, and MotA.

scholarly journals Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli

2002 ◽  
Vol 184 (23) ◽  
pp. 6730-6733 ◽  
Author(s):  
Sean M. O'Donnell ◽  
Gary R. Janssen
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Primer Extension ◽  
Ribosomal Subunits ◽  
Ribosome Binding ◽  
Initiation Factors ◽  
Translation Initiation Factors

ABSTRACT By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.

scholarly journals POLARITY IN SEGMENTS OF THE ESCHERICHIA COLI trp OPERON WITH DELETED INTRAOPERONIC TRANSLATIONAL INITIATION SIGNALS

Genetics ◽  
1975 ◽  
Vol 80 (4) ◽  
pp. 651-666
Author(s):  
Yasunobu Kano ◽  
Fumio Imamoto
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Nonsense Mutation ◽  
Messenger Rna ◽  
Distal Part ◽  
High Efficiency ◽  
Translational Initiation ◽  
Transcriptional Termination ◽  
Trp Operon

ABSTRACT The effect of deletion of the operator-distal genes of the trp operon, including the trpE-trpD intercistronic punctuation point, on the degree of transcriptional polarity (in this case the effect of a nonsense mutation on the level of mRNA from the distal part of the very gene where the mutation is located) was investigated. Double mutants which contain a nonsense mutation and a deletion in trpE were constructed, and the degree of transcriptional polarity was estimated by the decrease in messenger RNA for the operator-distal trpE beyond the nonsense mutation, as well as by the production of truncated messenger RNA for the region of trpE proximal to the nonsense mutation. The content of mRNA of operator-distal trpE and the size of the mRNA of operator-proximal trpE of the double mutants show that transcriptional polarity is not relaxed as a function of distance of the nonsense mutation from the operator-distal end of the trpE segment (at which the subsequent high efficiency translational initiation signal has been deleted). These findings are consistent with the conclusion that the degree of polarity depends on the distance of the nonsense mutation fro mthe subsequent translation initiation signal, but not on its distance from the operator-distal end, including possible translational or transcriptional termination signals

Sign in / Sign up

Export Citation Format

Share Document