Cloning and Expression of Two Genes Coding for Sodium Pumps in the Salt-Tolerant Yeast Debaryomyces hansenii
2001 ◽
Vol 183
(10)
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pp. 3251-3255
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Keyword(s):
High Ph
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ABSTRACT Two genes encoding Na+-ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designatedENA1 from D. hansenii (DhENA1) andDhENA2, exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na+ concentrations, while the expression of DhENA2 also required high pH. A mutant ofSaccharomyces cerevisiae lacking the Na+ efflux systems and sensitive to Na+, when transformed withDhENA1 or DhENA2, recovered Na+tolerance and also the ability to extrude Na+.
2007 ◽
Vol 7
(1)
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pp. 102-109
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1989 ◽
Vol 55
(12)
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pp. 3167-3172
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1992 ◽
Vol 267
(23)
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pp. 16371-16378
1997 ◽
Vol 136
(1)
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pp. 111-123
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Keyword(s):
2002 ◽
Vol 22
(20)
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pp. 6946-6948
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2004 ◽
Vol 26
(24)
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pp. 1861-1865
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1995 ◽
Vol 1264
(1)
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pp. 79-86
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Keyword(s):