Replacement of Tyrosine 181 by Phenylalanine in Gentisate 1,2-Dioxygenase I from Pseudomonas alcaligenes NCIMB 9867 Enhances Catalytic Activities
2005 ◽
Vol 187
(21)
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pp. 7543-7545
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Keyword(s):
ABSTRACT xlnE, encoding gentisate 1,2-dioxygenase (EC 1.13.11.4), from Pseudomonas alcaligenes (P25X) was mutagenized by site-directed mutagenesis. The mutant enzyme, Y181F, demonstrated 4-, 3-, 6-, and 16-fold increases in relative activity towards gentisate and 3-fluoro-, 4-methyl-, and 3-methylgentisate, respectively. The specific mutation conferred a 13-fold higher catalytic efficiency (k cat/Km ) on Y181F towards 3-methylgentisate than that of the wild-type enzyme.
2012 ◽
Vol 78
(11)
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pp. 3880-3884
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1990 ◽
Vol 112
(5)
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pp. 1983-1989
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2013 ◽
Vol 79
(13)
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pp. 4072-4077
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Kinetic Studies on CphA Mutants Reveal the Role of the P158-P172 Loop in Activity versus Carbapenems
2016 ◽
Vol 60
(5)
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pp. 3123-3126
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Keyword(s):
Zinc Ion
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