Green mussel (Perna viridis) and cockle shell (Anadara granosa) are one of many sources of animal<br />protein which is many cultivated in Indonesia because their price is relatively affordable. This study was<br />conducted to identify the presence of Listeria monocytogenes in 27 samples of green mussels and 3 samples<br />of cockle shells using real-time Polymerase Chain Reaction (real-time PCR) and biochemical methods. The<br />target gene for amplification in real-time PCR was an hlyA gene because this gene was a determinant of<br />virulence genes that produce listeriolysin O. Primers used in this study were forward primer DG69 (GTG<br />CCG GGT AAA AGA CCA TA) and reverse primer DG74 (CGC CAC TGA GAT ACT AT) and fluorescence<br />signals indicator using SYBR Green I. The results of analysis using real-time PCR were negative Listeria<br />monocytogenes in all samples, while using biochemical methods there was one of 30 samples contaminated<br />by Listeria welshimeri.
Comparison of Real-Time PCR and Conventional Biochemical Methods for Identification of Staphylococcus lugdunensis
