scholarly journals Development and Validation of a Negative-Strand-Specific Reverse Transcription-PCR Assay for Detection of a Chicken Strain of Hepatitis E Virus: Identification of Nonliver Replication Sites

2008 ◽  
Vol 46 (8) ◽  
pp. 2630-2634 ◽  
Author(s):  
P. Billam ◽  
F. W. Pierson ◽  
W. Li ◽  
T. LeRoith ◽  
R. B. Duncan ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Pcr Assay ◽  
Negative Strand ◽  
Virus Identification ◽  
Reverse Transcription Pcr ◽  
Replication Sites ◽  
Development And Validation

scholarly journals Evidence that the Genomic RNA of Hepatitis E Virus Is Capped

1999 ◽  
Vol 73 (10) ◽  
pp. 8848-8850 ◽  
Author(s):  
Yamina Kabrane-Lazizi ◽  
Xiang-Jin Meng ◽  
Robert H. Purcell ◽  
Suzanne U. Emerson
Keyword(s):  
Monoclonal Antibody ◽  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genomic Rna ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Replication Strategy ◽  
Positive Sense ◽  
Rna Genome

ABSTRACT Hepatitis E virus (HEV) is an unclassified virus with a positive-sense RNA genome and an undefined replication strategy. In order to determine whether the HEV genome is capped or not, we developed a reverse transcription-PCR assay that is based on the ability of a monoclonal antibody to recognize 7-methylguanosine (m7G). Antibody to m7G bound RNA extracted from virions of two different HEV genotypes. The cap analog competitively inhibited the binding of virion RNAs, demonstrating that HEV has a capped RNA genome.

scholarly journals Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses

Viruses ◽  
2014 ◽  
Vol 6 (10) ◽  
pp. 3893-3906 ◽  
Author(s):  
Decai Tuo ◽  
Wentao Shen ◽  
Yong Yang ◽  
Pu Yan ◽  
Xiaoying Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Development And Validation

scholarly journals Detection of Hepatitis E Virus RNA in Raw Cured Sausages and Raw Cured Sausages Containing Pig Liver at Retail Stores in Switzerland

2017 ◽  
Vol 81 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Petra Giannini ◽  
Marco Jermini ◽  
Lorenzo Leggeri ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan
Keyword(s):  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Food Product ◽  
Hepatitis E ◽  
Pig Liver ◽  
Viral Loads ◽  
Reverse Transcription Pcr ◽  
Potential Source ◽  
Virus Rna ◽  
Pork Sausages

ABSTRACT Hepatitis E virus (HEV) is the causative agent of an acute and self-limiting hepatitis and is increasingly detected in food products containing pork. In this study, 102 raw sausages containing pig liver (mortadella di fegato) and 18 raw pork sausages (salami type sausage) collected at retail level in a region of southern Switzerland were screened for the presence of HEV by quantitative real-time reverse transcription PCR. HEV was detected in 12 (11.8%) of 102 mortadella di fegato products but not in any of the salami sausages. Viral loads in the mortadella di fegato sausages ranged from log HEV 2.3 to 5.7 genome copies per gram of food product. This study identifies mortadella di fegato type sausages made with raw pig liver as a potential source of HEV infection in humans.

Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR.

1996 ◽  
Vol 34 (6) ◽  
pp. 1568-1571 ◽  
Author(s):  
S Turkoglu ◽  
Y Lazizi ◽  
H Meng ◽  
A Kordosi ◽  
P Dubreuil ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Reverse Transcription Pcr ◽  
Virus Rna
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