scholarly journals Evidence that the Genomic RNA of Hepatitis E Virus Is Capped

1999 ◽  
Vol 73 (10) ◽  
pp. 8848-8850 ◽  
Author(s):  
Yamina Kabrane-Lazizi ◽  
Xiang-Jin Meng ◽  
Robert H. Purcell ◽  
Suzanne U. Emerson
Keyword(s):  
Monoclonal Antibody ◽  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genomic Rna ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
Replication Strategy ◽  
Positive Sense ◽  
Rna Genome

ABSTRACT Hepatitis E virus (HEV) is an unclassified virus with a positive-sense RNA genome and an undefined replication strategy. In order to determine whether the HEV genome is capped or not, we developed a reverse transcription-PCR assay that is based on the ability of a monoclonal antibody to recognize 7-methylguanosine (m7G). Antibody to m7G bound RNA extracted from virions of two different HEV genotypes. The cap analog competitively inhibited the binding of virion RNAs, demonstrating that HEV has a capped RNA genome.

scholarly journals Activation of CXCL-8 Transcription by Hepatitis E Virus ORF-1 via AP-1

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Zhubing Li ◽  
Lu Chen ◽  
Qiang Liu
Keyword(s):  
Transcriptional Activation ◽  
Promoter Activity ◽  
Hepatitis E Virus ◽  
Rna Virus ◽  
Ectopic Expression ◽  
Hepatitis E ◽  
Genomic Rna ◽  
Host Interactions ◽  
Positive Sense ◽  
Binding Sequence

Hepatitis E virus (HEV) is a small nonenveloped single-stranded positive-sense RNA virus and is one of the major causes for acute hepatitis worldwide. CXCL-8 is a small multifunctional proinflammatory chemokine. It was reported recently that HEV infection significantly upregulates CXCL-8 gene expression. In this study, we investigated the mechanism of HEV-induced CXCL-8 transcriptional activation. Using CXCL-8 promoter reporters of different lengths ranging from −1400 to −173, we showed that −173 promoter has the highest promoter activity in the presence of HEV genomic RNA, indicating that the −173 promoter contains sequences responsible for CXCL-8 activation by HEV. Ectopic expression of the ORF-1 protein can upregulate the −173 CXCL-8 promoter activity. In contrast, expression of the ORF-2 protein suppresses the CXCL-8 promoter activity and expression of the ORF-3 protein has no effect on the CXCL-8 promoter activity. We further showed that AP-1 is required for CXCL-8 activation because neither HEV genomic RNA nor the ORF-1 protein can upregulate the −173 CXCL-8 promoter in the absence of the AP-1 binding sequence. Taken together, our results showed that HEV and HEV ORF-1 protein activate the CXCL-8 promoter via AP-1. This novel function of HEV ORF-1 protein should contribute to our understanding of HEV-host interactions and HEV-associated pathogenesis.

scholarly journals Detection of Hepatitis E Virus RNA in Raw Cured Sausages and Raw Cured Sausages Containing Pig Liver at Retail Stores in Switzerland

2017 ◽  
Vol 81 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Petra Giannini ◽  
Marco Jermini ◽  
Lorenzo Leggeri ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan
Keyword(s):  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Food Product ◽  
Hepatitis E ◽  
Pig Liver ◽  
Viral Loads ◽  
Reverse Transcription Pcr ◽  
Potential Source ◽  
Virus Rna ◽  
Pork Sausages

ABSTRACT Hepatitis E virus (HEV) is the causative agent of an acute and self-limiting hepatitis and is increasingly detected in food products containing pork. In this study, 102 raw sausages containing pig liver (mortadella di fegato) and 18 raw pork sausages (salami type sausage) collected at retail level in a region of southern Switzerland were screened for the presence of HEV by quantitative real-time reverse transcription PCR. HEV was detected in 12 (11.8%) of 102 mortadella di fegato products but not in any of the salami sausages. Viral loads in the mortadella di fegato sausages ranged from log HEV 2.3 to 5.7 genome copies per gram of food product. This study identifies mortadella di fegato type sausages made with raw pig liver as a potential source of HEV infection in humans.

Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR.

1996 ◽  
Vol 34 (6) ◽  
pp. 1568-1571 ◽  
Author(s):  
S Turkoglu ◽  
Y Lazizi ◽  
H Meng ◽  
A Kordosi ◽  
P Dubreuil ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Reverse Transcription Pcr ◽  
Virus Rna

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr
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