Identification and subtyping of avian influenza viruses by reverse transcription-PCR

2001 ◽  
Vol 97 (1-2) ◽  
pp. 13-22 ◽  
Author(s):  
Ming-Shiuh Lee ◽  
Poa-Chun Chang ◽  
Jui-Hung Shien ◽  
Ming-Chu Cheng ◽  
Happy K Shieh
Keyword(s):  
Avian Influenza ◽  
Reverse Transcription ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
Reverse Transcription Pcr

A reverse transcription-PCR for subtyping of the neuraminidase of avian influenza viruses

2009 ◽  
Vol 155 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Bao-Feng Qiu ◽  
Wu-Jie Liu ◽  
Da-Xin Peng ◽  
Shun-Lin Hu ◽  
Ying-Hua Tang ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Reverse Transcription ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
Reverse Transcription Pcr

scholarly journals A rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication

2008 ◽  
Vol 75 (4) ◽  
Author(s):  
C. Abolnik
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
South African ◽  
Reverse Transcription ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Influenza Viruses ◽  
Western Cape ◽  
Avian Influenza Viruses ◽  
Reverse Transcription Pcr

A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.

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