scholarly journals Genotyping of Chlamydophila psittaci by Real-Time PCR and High-Resolution Melt Analysis

2008 ◽  
Vol 47 (1) ◽  
pp. 175-181 ◽  
Author(s):  
S. L. Mitchell ◽  
B. J. Wolff ◽  
W. L. Thacker ◽  
P. G. Ciembor ◽  
C. R. Gregory ◽  
...  
2010 ◽  
Vol 48 (3) ◽  
pp. 697-702 ◽  
Author(s):  
Jonas M. Winchell ◽  
Bernard J. Wolff ◽  
Rebekah Tiller ◽  
Michael D. Bowen ◽  
Alex R. Hoffmaster

2010 ◽  
Vol 150 ◽  
pp. 127-127 ◽  
Author(s):  
Benjaporn Panichareon ◽  
Paisarn Khawsak ◽  
Warin Deesukon ◽  
Wasana Sukhumsirichart

2009 ◽  
Vol 15 (8) ◽  
pp. 756-762 ◽  
Author(s):  
S.B. Schwartz ◽  
K.A. Thurman ◽  
S.L. Mitchell ◽  
B.J. Wolff ◽  
J.M. Winchell

2015 ◽  
Vol 59 (9) ◽  
pp. 5574-5580 ◽  
Author(s):  
Peera Hemarajata ◽  
Shangxin Yang ◽  
Janet A. Hindler ◽  
Romney M. Humphries

ABSTRACTThe rapid global spread of carbapenem-resistantEnterobacteriaceae(CRE) poses an urgent threat to public health. More than 250 class D β-lactamases (OXAs) have been described in recent years, with variations in hydrolytic activity for β-lactams. The plasmid-borne OXA-48 β-lactamase and its variants are identified only sporadically in the United States but are common in Europe. Recognition of these OXA-48-like carbapenemases is vital in order to control their dissemination. We developed a real-time PCR assay based on high-resolution melt analysis, usingblaOXA-48-like-specific primers coupled with an unlabeled 3′-phosphorylated oligonucleotide probe (LunaProbe) homologous to OXA-48-like carbapenemase genes. The assay was validated using genomic DNA from 48 clinical isolates carrying a variety of carbapenemase genes, includingblaKPC,blaSME,blaIMP,blaNDM-1,blaVIM,blaOXA-48,blaOXA-162,blaOXA-181,blaOXA-204,blaOXA-244,blaOXA-245, andblaOXA-232. Our assay identified the presence ofblaOXA-48-likeβ-lactamase genes and clearly distinguished betweenblaOXA-48and its variants in control strains, including betweenblaOXA-181andblaOXA-232, which differ by only a single base pair in the assay target region. This approach has potential for use in epidemiological investigations and continuous surveillance to help control the spread of CRE strains producing OXA-48-like enzymes.


Lab on a Chip ◽  
2016 ◽  
Vol 16 (18) ◽  
pp. 3524-3531 ◽  
Author(s):  
A. Sposito ◽  
V. Hoang ◽  
D. L. DeVoe

A disposable thermoplastic microfluidic platform enabling rapid point-of-care PCR-based diagnostics with automated sample loading is reported.


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