Partial atlE Sequencing of Staphylococcus epidermidis Strains from Prosthetic Joint Infections

There is increased awareness of the worldwide spread of specific epidemic multidrug-resistant (MDR) lineages of the human commensal Staphylococcus epidermidis . Here, using bioinformatic analyses accounting for population structure, we determined genomic traits (genes, SNPs and k-mers) that distinguish S. epidermidis causing prosthetic-joint infections (PJIs) from commensal isolates from nares, by analysing whole-genome sequencing data from S. epidermidis from PJIs prospectively collected over 10 years in Sweden, and contemporary S. epidermidis from the nares of patients scheduled for arthroplasty surgery. Previously suggested virulence determinants and the presence of genes and mutations linked to antimicrobial resistance (AMR) were also investigated. Publicly available S. epidermidis sequences were used for international extrapolation and validation of findings. Our data show that S. epidermidis causing PJIs differed from nasal isolates not by virulence but by traits associated with resistance to compounds used in prevention of PJIs: β-lactams, aminoglycosides and chlorhexidine. Almost a quarter of the PJI isolates did not belong to any of the previously described major nosocomial lineages, but the AMR-related traits were also over-represented in these isolates, as well as in international S. epidermidis isolates originating from PJIs. Genes previously associated with virulence in S. epidermidis were over-represented in individual lineages, but failed to reach statistical significance when adjusted for population structure. Our findings suggest that the current strategies for prevention of PJIs select for nosocomial MDR S. epidermidis lineages that have arisen from horizontal gene transfer of AMR-related traits into multiple genetic backgrounds.

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Lower activation of caspase-1 by Staphylococcus epidermidis isolated from prosthetic joint infections compared to commensals

Journal of Bone and Joint Infection ◽

10.7150/jbji.21567 ◽

2018 ◽

Vol 3 (1) ◽

pp. 10-14 ◽

Cited By ~ 1

Author(s):

Emeli Månsson ◽

Berolla Sahdo ◽

Åsa Nilsdotter-Augustinsson ◽

Eva Särndahl ◽

Bo Söderquist

Keyword(s):

Staphylococcus Epidermidis ◽

Normal Skin ◽

Human Neutrophils ◽

Caspase 1 ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections ◽

Skin Flora ◽

Number Of Individuals ◽

Active Caspase

Abstract. Nosocomial sequence types of Staphylococcus epidermidis dominate in prosthetic joint infections. We examined caspase-1 activation in human neutrophils after incubation with Staphylococcus epidermidis isolated from prosthetic joint infections and normal skin flora. Active caspase-1 was lower after incubation with isolates from prosthetic joint infections than after incubation with commensal isolates. Both host and isolate dependent differences in active caspase-1 were noted. Our results indicate that there might be a host-dependent incapacity to elicit a strong caspase-1 response towards certain strains of S. epidermidis. Further experiments with a larger number of individuals are warranted.

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Bacterial Extracellular DNA Production Is Associated with Outcome of Prosthetic Joint Infections

BioMed Research International ◽

10.1155/2018/1067413 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Beata Zatorska ◽

Carla Renata Arciola ◽

Nicolas Haffner ◽

Luigi Segagni Lusignani ◽

Elisabeth Presterl ◽

...

Keyword(s):

Clinical Outcome ◽

Staphylococcus Epidermidis ◽

Joint Infection ◽

Surrogate Marker ◽

Predictive Marker ◽

Extracellular Dna ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections ◽

Dna Production

In a retrospective study the association of the production of extracellular DNA (eDNA) in biofilms of clinical staphylococcal isolates from 60 patients with prosthetic joint infection (PJI) and the clinical outcome were investigated. Data from a previous study on eDNA production determined in 24-hour biofilms of staphylococcal isolates (Staphylococcus aureusn=30,Staphylococcus epidermidisn=30) was correlated with the patients’ clinical outcome after 3 and 12 months. Statistical analysis was performed using either the Spearman’s rank correlations test or the t-test. eDNA production ofS. epidermidisin 24-hour biofilms correlated with the patients’ outcome ‘not cured‘ after 12 months. ForS. aureusno such correlation was detected. Thus, eDNA may be a virulence factor ofS. epidermidis.Quantification of eDNA production as a surrogate marker for biofilm formation might be a potential predictive marker for the management of PJI.

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Staphylococcus epidermidis from prosthetic joint infections induces lower IL-1β release from human neutrophils than isolates from normal flora

Apmis ◽

10.1111/apm.12861 ◽

2018 ◽

Vol 126 (8) ◽

pp. 678-684

Author(s):

Emeli Månsson ◽

Bo Söderquist ◽

Åsa Nilsdotter-Augustinsson ◽

Eva Särndahl ◽

Isak Demirel

Keyword(s):

Staphylococcus Epidermidis ◽

Human Neutrophils ◽

Normal Flora ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections

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Pathogenesis of Staphylococcus epidermidis in prosthetic joint infections: can identification of virulence genes differentiate between infecting and commensal strains?

Journal of Hospital Infection ◽

10.1016/j.jhin.2020.04.026 ◽

2020 ◽

Vol 105 (3) ◽

pp. 561-568

Author(s):

A. Sánchez ◽

N. Benito ◽

A. Rivera ◽

L. García ◽

E. Miró ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Virulence Genes ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections

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Genotypic and phenotypic changes of Staphylococcus epidermidis during relapse episodes in prosthetic joint infections

Brazilian Journal of Microbiology ◽

10.1007/s42770-019-00190-3 ◽

2019 ◽

Vol 51 (2) ◽

pp. 601-612

Author(s):

Silvestre Ortega-Peña ◽

Rafael Franco-Cendejas ◽

Alejandra Aquino-Andrade ◽

Gabriel Betanzos-Cabrera ◽

Ashutosh Sharma ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Phenotypic Changes ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections

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Staphylococcus epidermidis isolates from nares and prosthetic joint infections are mupirocin susceptible

Journal of Bone and Joint Infection ◽

10.7150/jbji.22459 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Lavin Salih ◽

Staffan Tevell ◽

Emeli Månsson ◽

Åsa Nilsdotter-Augustinsson ◽

Bengt Hellmark ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Staphylococcus Epidermidis ◽

Antimicrobial Agents ◽

Multi Drug Resistance ◽

Disc Diffusion ◽

Diffusion Test ◽

Joint Replacements ◽

Joint Infections ◽

Prosthetic Joint ◽

Prosthetic Joint Infections

Abstract. The objective of the present study was to investigate the antibiotic susceptibility including mupirocin among Staphylococcus. epidermidis isolated from prosthetic joint infections (PJIs) (n=183) and nasal isolates (n=75) from patients intended to undergo prosthetic joint replacements. Susceptibility to mupirocin (used for eradication of nasal carriership of Staphylococcus aureus) was investigated by gradient test, and susceptibility to various other antimicrobial agents was investigated by disc diffusion test. All isolates, except three from PJIs and one from the nares, were fully susceptible to mupirocin. Multi-drug resistance (≥3 antibiotic classes) was found in 154/183 (84.2%) of the PJI isolates but only in 2/75 (2.7%) of the nares isolates, indicating that S. epidermidis causing PJIs do not originate from the nares.

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