Comparison of the hemagglutination inhibition procedure and an enzyme-linked immunosorbent assay for detection of specific antibodies to pneumonia virus of mice in experimentally infected laboratory rats.

1980 ◽  
Vol 11 (2) ◽  
pp. 162-166 ◽  
Author(s):  
J P Descôteaux ◽  
P Payment ◽  
M Trudel
Keyword(s):  
Immunosorbent Assay ◽  
Hemagglutination Inhibition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Laboratory Rats

scholarly journals Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

1998 ◽  
Vol 5 (5) ◽  
pp. 613-616 ◽  
Author(s):  
Felix Grimm ◽  
Friedrich E. Maly ◽  
Jian Lü ◽  
Roberto Llano
Keyword(s):  
Healthy Volunteers ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Igg Subclass ◽  
Specific Antibodies ◽  
Specific Igg4 ◽  
Antibody Levels

ABSTRACT The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

An Enzyme-Linked Immunosorbent Assay for Protein Kinase D Activity Using Phosphorylation Site-Specific Antibodies

2007 ◽  
Vol 5 (5) ◽  
pp. 637-644 ◽  
Author(s):  
An Rykx ◽  
Sadia Vancauwenbergh ◽  
Line De Kimpe ◽  
Katrien Janssens ◽  
Sandy Vandoninck ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Immunosorbent Assay ◽  
Phosphorylation Site ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Kinase D ◽  
Site Specific ◽  
Specific Antibodies
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