Evaluation of spot CAMP test for identification of group B streptococci.

1986 ◽  
Vol 24 (2) ◽  
pp. 296-297 ◽  
Author(s):  
H B Ratner ◽  
L S Weeks ◽  
C W Stratton
Keyword(s):  
Group B Streptococci ◽  
Camp Test ◽  
Group B

scholarly journals Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping

1978 ◽  
Vol 8 (5) ◽  
pp. 480-488
Author(s):  
S M Gubash
Keyword(s):  
Human Blood ◽  
Test Group ◽  
Blood Agar ◽  
Group A Streptococci ◽  
Alpha Toxin ◽  
Group B Streptococci ◽  
Camp Factor ◽  
Group A ◽  
Camp Test ◽  
Group B

A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shaped zone of hemolysis; 74% of group A gave a crescent-shaped lytic zone, whereas all isolates of groups C and G and the remaining 26% of group A streptococci gave a bullet-shaped lytic zone. By comparison, in the CAMP test incubated aerobically and anaerobically, 70 and 91%, respectively, of streptococci other than group B gave positive, arrowhead-shaped lytic zones. If all intermediate positive reactions in the CAMP tests were read as negative after aerobic incubation, only 89% of group B streptococci would be properly identified. The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups.

scholarly journals CAMP-disk test for presumptive identification of group B streptococci

1977 ◽  
Vol 6 (1) ◽  
pp. 42-45
Author(s):  
H W Wilkinson
Keyword(s):  
Agar Plate ◽  
Blood Agar Plate ◽  
Complete Agreement ◽  
Double Blind Study ◽  
Group B Streptococci ◽  
Double Blind ◽  
Sheep Blood Agar Plate ◽  
Camp Test ◽  
Group B ◽  
Disk Test

The modification of the CAMP test for goup B streptococci involved substituting a paper disk impregnated with partially purified beta-hemolysin for the staphylococcal culture that was the source of beta-hemolysin in the original test. The disk is placed onto a sheep blood agar plate beside the streak of Streptococcus being tested. The plate is then incubated aerobically at 35 degrees C. A positive reaction consists of a lunar-shaped clear zone that appears within 24 h in the dark beta-hemolysin zone surrounding the disk. A double-blind study of 135 randomly coded streptococcal isolates showed complete agreement between the CAMP-disk test and the standard Lancefield precipitin test. All group B streptococci tested had positive reactions, and all strains tested from streptococcal groups A, C, D, and G were negative. The CAMP-disk test is a simple and convenient way to identify presumptively group B streptococci.

Modified Christie–Atkins–Munch-Petersen (CAMP) test for direct identification of hemolytic and nonhemolytic group B streptococci on primary plating

1980 ◽  
Vol 26 (4) ◽  
pp. 539-542 ◽  
Author(s):  
Ben H. C. Bae ◽  
Edward J. Bottone
Keyword(s):  
Sheep Blood Agar ◽  
Group B Streptococci ◽  
Direct Identification ◽  
Sheep Erythrocytes ◽  
Active Edge ◽  
Sheep Blood ◽  
Microbial Species ◽  
Camp Test ◽  
Group B ◽  
Direct Recognition

A primary plating medium, using sheep blood agar with preformed zones of cell-free staphylococcal β-hemolysin, was used for direct identification of both hemolytic and nonhemolytic group B streptococci by the direct elicitation of the Christie–Atkins–Munch-Petersen (CAMP) reaction. A positive CAMP reaction consisted of a semilunar-shaped area or circle of complete hemolysis of sheep erythrocytes and occurred only when colonies of group B streptococci developed along the periphery of the preformed zone of staphylococcal β-hemolysin. Of 104 simulated specimens containing group B streptococci in combination with a variety of microbial species and of 85 vaginal specimens examined, direct recognition of group B streptococci was possible in 64 (61.5%) simulated specimens and in 12 (100%) vaginal cultures. Group B streptococcal colonies not developing along the active edge of staphylococcal β-hemolysin were still identified by subculture. Of particular value was the facilitated recognition of colonies of nonhemolytic group B streptococcal strains and of hemolytic group B streptococci few in number or overgrown by other microbial species.

scholarly journals Multiple-Inocula (Replicator) CAMP Test for Presumptive Identification of Group B Streptococci

1978 ◽  
Vol 7 (2) ◽  
pp. 232-233
Author(s):  
Peter C. Fuchs ◽  
Carol Christy ◽  
Ronald N. Jones
Keyword(s):  
Group B Streptococci ◽  
Presumptive Identification ◽  
Camp Test ◽  
Group B

A replicator method for performing the CAMP test is described. Of 304 group B streptococci tested, 303 (99.7%) were CAMP positive. None of 1,093 non-group B streptococci was CAMP positive.

Evaluation of the Spot-CAMP Test for the Rapid Presumptive Identification of Group B Streptococci

1985 ◽  
Vol 84 (2) ◽  
pp. 216-219 ◽  
Author(s):  
Joseph R. DiPersio ◽  
Jean E. Barrett ◽  
Raymond L. Kaplan
Keyword(s):  
Group B Streptococci ◽  
Presumptive Identification ◽  
Camp Test ◽  
Group B

Susceptibility to Six Antibiotics of Group B Streptococci Isolated from Cerebrospinal Fluid

1985 ◽  
Vol 17 (2) ◽  
pp. 191-193
Author(s):  
Chris Mulder ◽  
Pieter Bol ◽  
Arjan Nabbe ◽  
Bob Zanen
Keyword(s):  
Cerebrospinal Fluid ◽  
Group B Streptococci ◽  
Group B

scholarly journals Influence of group B streptococci on piglet pulmonary artery response to bradykinin

1999 ◽  
Vol 86 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Richard M. Whitehurst ◽  
Rachel Laskey ◽  
Ronald N. Goldberg ◽  
Donald Herbert ◽  
Cornelius Van Breemen
Keyword(s):  
Pulmonary Artery ◽  
Contractile Response ◽  
Isometric Force ◽  
Control Group ◽  
Relaxed Controls ◽  
Vascular Response ◽  
Group B Streptococci ◽  
Resting Tension ◽  
Neonatal Group ◽  
Group B

To study whether a sepsis-induced increase in des-Arg9-bradykinin (des-Arg9-BK) and bradykinin (BK) B1-receptor activity participates in the observed increase in pulmonary vascular resistance in neonatal group B streptococcal sepsis (GBS), isometric force bioassays of pulmonary artery (PA) rings were studied, after 4-h exposure to either Krebs or GBS, by using the following protocols: 1) BK dose-response curve, 2) vascular response to BK with N G-nitro-l-arginine methyl ester (l-NAME), and 3) response to des-Arg9-BK (BK metabolite and B1 agonist). PA rings exposed to BK resulted in contraction in the GBS group and a decrease in resting tension in the Control group ( P = 0.034) at a concentration of 10−5 M. GBS-treated PA rings contracted more to des-Arg9-BK than did Controls ( P < 0.001). BK (10−6 M) relaxed preconstricted PA rings incubated in GBS less than BK relaxed Controls ( P < 0.001), and preincubation withl-NAME decreased relaxation in both. These results suggest that GBS decreased endothelium-dependent BK relaxation and increased contractile response to des-Arg9-BK. We speculate that this occurs secondary to upregulation of B1 receptors reflected by B1-agonist-mediated PA contraction.

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