Polymerase chain reaction for Group B Streptococci (GBS) at labor highly correlates with vaginal GBS load

2021 ◽  
pp. 1-5
Author(s):  
Mohammed Rohi Khalil ◽  
Poul Bak Thorsen ◽  
Jens Kjølseth Møller ◽  
Niels Uldbjerg
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Group B Streptococci ◽  
Polymerase Chain ◽  
Group B

Rapid Group B Streptococci Screening Using a Real-Time Polymerase Chain Reaction Assay

2008 ◽  
Vol 111 (6) ◽  
pp. 1335-1341 ◽  
Author(s):  
Rodney K. Edwards ◽  
Susan M. Novak-Weekley ◽  
Patrick P. Koty ◽  
Thomas Davis ◽  
Leroy J. Leeds ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Chain Reaction ◽  
Group B Streptococci ◽  
Polymerase Chain ◽  
Group B

Perinatal Screening for Group B Streptococci: Cost-Benefit Analysis of Rapid Polymerase Chain Reaction

PEDIATRICS ◽  
2002 ◽  
Vol 110 (3) ◽  
pp. 471-480 ◽  
Author(s):  
C. A. Haberland ◽  
W. E. Benitz ◽  
G. D. Sanders ◽  
J. B. Pietzsch ◽  
S. Yamada ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cost Benefit Analysis ◽  
Cost Benefit ◽  
Benefit Analysis ◽  
Chain Reaction ◽  
Group B Streptococci ◽  
Rapid Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Group B

scholarly journals Comparison of Three Rapid Commercial Canine Parvovirus Antigen Detection Tests with Electron Microscopy and Polymerase Chain Reaction

2009 ◽  
Vol 21 (3) ◽  
pp. 344-345 ◽  
Author(s):  
Silke Schmitz ◽  
Christina Coenen ◽  
König Matthias ◽  
Thiel Heinz-Jürgen ◽  
Reto Neiger
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Chronic Diarrhea ◽  
Gastrointestinal Disease ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Chain Reaction ◽  
Group A ◽  
Polymerase Chain ◽  
Group B

Different antibody-based tests for rapid detection of Canine parvovirus antigens in feces are commercially available, allowing quick diagnosis in a clinical setting. However, the diagnostic accuracy of these tests compared with standard methods has not been evaluated so far. In the current study, 3 commercial tests were compared with immune-electron microscopy (IEM) and polymerase chain reaction (PCR). Dogs were divided into 3 groups: group A, samples from dogs with acute hemorrhagic diarrhea ( n = 50); group B, dogs with chronic diarrhea ( n = 10); and group C, dogs with no evidence of gastrointestinal disease ( n = 40). Specificity of all 3 commercial tests versus PCR and IEM was good to excellent (92.2–100%). Sensitivity, in contrast, was poor: 15.8–26.3% versus PCR and 50–60% versus IEM. In group A, 10 dogs were positive by IEM and 24 dogs were positive by PCR. Positive PCR results were also obtained from animals in control groups (group B, 1 dog; group C, 5 dogs). No dog in group B or C was positive by IEM. In conclusion, the rapid tests are useful to diagnose canine parvoviral enteritis, but they do not rule out parvovirus infection in an animal with typical clinical signs. In addition, a small percentage of healthy dogs and dogs with chronic diarrhea showed positive PCR results; this may be due to asymptomatic/persistent infection or intestinal passage of virus. The significance of this finding remains unclear.

Clinical value of polymerase chain reaction in detecting group B streptococcus during labor

2017 ◽  
Vol 43 (6) ◽  
pp. 996-1000 ◽  
Author(s):  
Dorothea Maria Koppes ◽  
Antonius Arnoldus Cornelis Maria Vriends ◽  
Michiel van Rijn ◽  
Antonine Dimphne van Heesewijk
Keyword(s):  
Polymerase Chain Reaction ◽  
Group B Streptococcus ◽  
Chain Reaction ◽  
Clinical Value ◽  
Polymerase Chain ◽  
Group B

Detection of group B and C rotaviruses by polymerase chain reaction.

1991 ◽  
Vol 29 (3) ◽  
pp. 519-523 ◽  
Author(s):  
V Gouvea ◽  
J R Allen ◽  
R I Glass ◽  
Z Y Fang ◽  
M Bremont ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Group B

scholarly journals Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

2018 ◽  
Vol 5 (7) ◽  
Author(s):  
Ameneh Khatami ◽  
Tara M Randis ◽  
Anna Chamby ◽  
Thomas A Hooven ◽  
Margaret Gegick ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Consensus Sequence ◽  
Group B Streptococcus ◽  
Improved Method ◽  
Chain Reaction ◽  
Consensus Sequences ◽  
Conserved Genes ◽  
Culture Independent ◽  
Polymerase Chain ◽  
Group B

Abstract Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

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