scholarly journals Antibiotic resistance patterns of group B streptococci in pregnant women.

1990 ◽  
Vol 28 (1) ◽  
pp. 5-7 ◽  
Author(s):  
K Berkowitz ◽  
J A Regan ◽  
E Greenberg
Keyword(s):  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Group B Streptococci ◽  
Resistance Patterns ◽  
Group B ◽  
Antibiotic Resistance Patterns

scholarly journals Distribution of Pilus island and antibiotic resistance genes in Streptococcus agalactiae obtained from vagina of pregnant women in Yazd, Iran

2020 ◽  
Author(s):  
Mahdieh Nabavinia ◽  
Mohammad Bagher Khalili ◽  
Maryam Sadeh ◽  
Gilda Eslami ◽  
Mahmood Vakili ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Streptococcus Agalactiae ◽  
Antibiotic Resistance Genes ◽  
Invasive Disease ◽  
Group B Streptococci ◽  
Resistance Factors ◽  
Group B

Background and Objectives: Due to the important role of Streptococcus agalactiae, Group B streptococci (GBS), in production of invasive disease in neonates, investigation regarding the pathogenicity and antibiotic resistance factors is necessary in selecting the appropriate therapeutic agents. Beside capsule, the pilus has been currently recognized as an important factor in enhancing the pathogenicity of GBS. Resistance of GBS to selected antibiotics is noticeably increasing which is mainly due to the anomalous use of these drugs for treatment. The aim of this study was to determine the prevalence of pili genes followed by antibiotic susceptibility of GBS, previously serotyped, isolated from pregnant women in the city of Yazd, Iran. Materials and Methods: Fifty seven GBS from pregnant women were subjected to multiplex PCR for determination of PI-1, PI-2a and PI-2b pilus-islands and simultaneously, the phenotype of antibiotic resistance to penicillin, tetracycline, erythromycin, clindamycin, gentamycin and levofloxacin was determined. Antibiotic resistance genes (ermA, ermB, mefA, tetM, int-Tn) were further diagnosed using PCR and multiplex PCR. Results: PI-1+PI-2a with 71.9%; followed by PI-2a (21.1%) and PI-2b (7%) were observed. PI-1+PI-2a in serotype III was (73.2%), serotype II, Ia, Ib and V were 12.2%, 9.8%, 2.4% and 2.4% respectively. GBS penicillin sensitive was 89.5% and 96.5% resistance to tetracycline. The frequency of resistance genes were as follows: tetM (93%), ermA (33.3%), ermB (8.8%), int-Tn (80.7%) and mefA (0). Conclusion: Majority of GBS contained PI-1+PI-2a. Hence presence of this pilus stabilizes the colonization, therefore designing a program for diagnosing and treatment of infected pregnant women seems to be necessary.

scholarly journals Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Akua Obeng Forson ◽  
Wilson Bright Tsidi ◽  
David Nana-Adjei ◽  
Marjorie Ntiwaa Quarchie ◽  
Noah Obeng-Nkrumah
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Virulence Factors ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Carriage of Group B Streptococci in Pregnant Women from the Region of Krakow and their Antibiotic Resistance in the Year 2008–2012

2013 ◽  
Vol 62 (4) ◽  
pp. 427-433 ◽  
Author(s):  
MONIKA BRZYCHCZY-WŁOCH ◽  
DOROTA OCHOŃSKA ◽  
MAŁGORZATA BULANDA
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Retrospective Analysis ◽  
Group B Streptococci ◽  
Epidemiological Analysis ◽  
Average Value ◽  
Diagnostic Standards ◽  
Group B

The aim of the study was a retrospective analysis of the frequency of group B streptococci (Streptococcus agalactaie; GBS) carriage in pregnant women from the region of Krakow, together with an analysis of their drug resistance, carried out between 2008-2012. The study included 3363 pregnant women between 35 and 37 weeks of gestation, studied in accordance with the guidelines of the Polish Gynecological Society (2008). A high percentage of pregnant women who are carriers of group B streptococci was demonstrated. Each year covered by the study, it was in the range of 25-30%, with an average value equal to 28%. The results confirm the need for taking swabs from both the vagina and anus, since 15% of GBS-positive patients showed only rectal carriage. High percentage of isolates resistant to erythromycin was detected, which ranged from 22% to 29%, with an average value equal to 25%, as well as a high proportion of isolates resistant to clindamycin being 17-25%, with an average of 20%. The results indicate the need to standardize the methodology of collecting samples for GBS testing and introduce microbiological diagnostic standards in all gynecological and obstetric centers in Poland, in order to carry out a detailed epidemiological analysis in our country.

scholarly journals Group BStreptococcusVaginal Carriage in Pregnant Women as Deciphered by Clustered Regularly Interspaced Short Palindromic Repeat Analysis

2018 ◽  
Vol 56 (6) ◽  
Author(s):  
Clemence Beauruelle ◽  
Adeline Pastuszka ◽  
Laurent Mereghetti ◽  
Philippe Lanotte
Keyword(s):  
Pregnant Women ◽  
Rflp Analysis ◽  
Vaginal Swab ◽  
Capsular Type ◽  
Crispr Locus ◽  
Content Type ◽  
Crispr Array ◽  
Resistance Patterns ◽  
Group B ◽  
Antibiotic Resistance Patterns

ABSTRACTWe evaluated the diversity of group BStreptococcus(GBS) vaginal carriage populations in pregnant women. For this purpose, we studied each isolate present in a primary culture of a vaginal swab using a new approach based on clustered regularly interspaced short palindromic repeats (CRISPR) locus analysis. To evaluate the CRISPR array composition rapidly, a restriction fragment length polymorphism (RFLP) analysis was performed. For each different pattern observed, the CRISPR array was sequenced and capsular typing and multilocus sequence typing (MLST) were performed. A total of 970 isolates from 10 women were analyzed by CRISPR-RFLP. Each woman carrying GBS isolates presented one to five specific “personal” patterns. Five women showed similar isolates with specific and unique restriction patterns, suggesting the carriage of a single GBS clone. Different patterns were observed among isolates from the other five women. For three of these, CRISPR locus sequencing highlighted low levels of internal modifications in the locus backbone, whereas there were high levels of modifications for the last two women, suggesting the carriage of two different clones. These two clones were closely related, having the same ancestral spacer(s), the same capsular type and, in one case, the same ST, but showed different antibiotic resistance patterns in pairs. Eight of 10 women were colonized by a single GBS clone, while two of them were colonized by two strains, leading to a risk of selection of more-virulent and/or more-resistant clones during antibiotic prophylaxis. This CRISPR analysis made it possible to separate isolates belonging to a single capsular type and sequence type, highlighting the greater discriminating power of this approach.

scholarly journals Correction to: Escherichia coli bacteriuria in pregnant women in Ghana: antibiotic resistance patterns and virulence factors

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Akua Obeng Forson ◽  
Wilson Bright Tsidi ◽  
David Nana-Adjei ◽  
Marjorie Ntiwaa Quarchie ◽  
Noah Obeng-Nkrumah
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Virulence Factors ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns
Sign in / Sign up

Export Citation Format

Share Document