scholarly journals Simple disk-plate method for the biochemical confirmation of pathogenic Neisseria

1976 ◽  
Vol 3 (2) ◽  
pp. 172-174
Author(s):  
J A Valu
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Limited Sample ◽  
Plate Method ◽  
Simple Method ◽  
Carbohydrate Fermentation ◽  
Fermentation Test

The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement between the two methods when a limited sample of clinical isolates of Neisseria gonorrhoeae and Nesseria meningitidis were tested.

scholarly journals A simple carbohydrate fermentation test for identification of the pathogenic Neisseria

1975 ◽  
Vol 2 (1) ◽  
pp. 72-73
Author(s):  
A Reddick
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Mueller Hinton ◽  
Paper Disk ◽  
Carbohydrate Fermentation ◽  
Fermentation Test ◽  
Agar Method ◽  
Disk Method ◽  
Mueller Hinton Broth ◽  
Simple Carbohydrate

The carbohydrate fermentation test in cystine-Trypticase agar-tubed medium was compared with the Minitek system with carbohydrate-impregnated paper disks in Müeller-Hinton broth for identification of Neisseria gonorrhoeae and N. meningitidis. There was 100% agreement between the methods for confirmation of N. meningitidis. The paper disk method confirmed 98% of the N. gonorrhoeae isolates; the cystine-Trypticase agar method confirmed 96%. Reactions with the paper disk method could be read in 4 h.

scholarly journals Neisseria confirmation by an enriched, bicarbonate-containing carbohydrate medium

1978 ◽  
Vol 8 (5) ◽  
pp. 525-528
Author(s):  
J O Graves ◽  
L A Magee
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Related Species ◽  
Fermentation Medium ◽  
Clinical Isolates ◽  
Sheep Erythrocytes ◽  
Neisseria Species ◽  
Sugar Fermentation ◽  
Fermentation Test ◽  
Species Performance

A sugar fermentation medium for the confirmation of Neisseria and related species was developed. The medium contained a commercial supplement and a hemoglobin source prepared from lysed sheep erythrocytes. Bicarbonate in the medium substituted for a CO2-supplemented atmosphere. The medium was dispensed into screw-capped tubes. This medium was compared to cystine-Trypticase agar and the modified rapid fermentation test in the confirmation of Neisseria species. Performance of the new medium was equivalent to that of the modified rapid fermentation test, but cystine-Trypticase agar failed to confirm a significant number of clinical isolates of Neisseria gonorrhoeae.

scholarly journals Simplified complete medium for the growth of Neisseria gonorrhoeae

1977 ◽  
Vol 5 (1) ◽  
pp. 9-14
Author(s):  
R T Jones ◽  
R S Talley
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Growth Yields ◽  
Complete Medium ◽  
Maximal Growth ◽  
Plate Method ◽  
Commercial Media ◽  
Solid Media ◽  
Plate Counts ◽  
Commercial Medium

A complete medium for the growth of Neisseria gonorrhoeae has been developed, using the same basic ingredients contained in commercial media but with fewer supplements. Based on a comparison of plating efficiencies, this medium (designated TTU Complete Medium) supported the growth of laboratory strains of N. gonorrhoeae equal to that obtained on commercial GC agar base medium supplemented with Iso VitaleX. It was also equivalent to the commercial medium in supporting the growth of 67 clinical isolates and 6 auxotypes of N. gonorrhoeae when tested by the streak plate method. Based on turbidity measurements and viable plate counts, the liquid equivalent of TTU Complete Medium supported maximal growth yields of N. gonorrhoeae. The toxicity of different brands of agar appeared to be a major factor in preventing the growth of N. gonorrhoeae strains on solid media. The addition of starch neutralized the toxicity of some types of agar but not of others.

scholarly journals Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

2002 ◽  
Vol 46 (12) ◽  
pp. 3744-3749 ◽  
Author(s):  
Satoshi Ameyama ◽  
Shoichi Onodera ◽  
Masahiro Takahata ◽  
Shinzaburo Minami ◽  
Nobuko Maki ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Penicillin Binding Protein ◽  
Gene Sequences ◽  
Neisseria Flavescens ◽  
Neisseria Perflava

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

Resistance, molecular characterization and viability of Neisseria gonorrhoeae recent clinical isolates

2021 ◽  
Vol 156 (5) ◽  
pp. 249-250
Author(s):  
Elizabeth Calatrava-Hernández ◽  
Carla Foronda-García-Hidalgo ◽  
José Gutiérrez-Fernández
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Characterization ◽  
Clinical Isolates

scholarly journals Amino Acid Substitutions in Mosaic Penicillin-Binding Protein 2 Associated with Reduced Susceptibility to Cefixime in Clinical Isolates of Neisseria gonorrhoeae

2006 ◽  
Vol 50 (11) ◽  
pp. 3638-3645 ◽  
Author(s):  
Sho Takahata ◽  
Nami Senju ◽  
Yumi Osaki ◽  
Takuji Yoshida ◽  
Takashi Ida
Keyword(s):  
Amino Acid ◽  
Neisseria Gonorrhoeae ◽  
Molecular Mechanisms ◽  
Binding Protein ◽  
Complete Sequence ◽  
Clinical Isolates ◽  
Amino Acid Substitutions ◽  
Penicillin Binding Protein ◽  
Fourfold Increase ◽  
Genes Encoding

ABSTRACT The molecular mechanisms of reduced susceptibility to cefixime in clinical isolates of Neisseria gonorrhoeae, particularly amino acid substitutions in mosaic penicillin-binding protein 2 (PBP2), were examined. The complete sequence of ponA, penA, and por genes, encoding, respectively, PBP1, PBP2, and porin, were determined for 58 strains isolated in 2002 from Japan. Replacement of leucine 421 by proline in PBP1 and the mosaic-like structure of PBP2 were detected in 48 strains (82.8%) and 28 strains (48.3%), respectively. The presence of mosaic PBP2 was the main cause of the elevated cefixime MIC (4- to 64-fold). In order to identify the mutations responsible for the reduced susceptibility to cefixime in isolates with mosaic PBP2, penA genes with various mutations were transferred to a susceptible strain by genetic transformation. The susceptibility of partial recombinants and site-directed mutants revealed that the replacement of glycine 545 by serine (G545S) was the primary mutation, which led to a two- to fourfold increase in resistance to cephems. Replacement of isoleucine 312 by methionine (I312M) and valine 316 by threonine (V316T), in the presence of the G545S mutation, reduced susceptibility to cefixime, ceftibuten, and cefpodoxime by an additional fourfold. Therefore, three mutations (G545S, I312M, and V316T) in mosaic PBP2 were identified as the amino acid substitutions responsible for reduced susceptibility to cefixime in N. gonorrhoeae.

Evaluation of Susceptibilities to Carbapenems and Faropenem Against Cephalosporin-Resistant Neisseria gonorrhoeae Clinical Isolates with penA Mosaic Alleles

2019 ◽  
Vol 25 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Yoshiki Hiyama ◽  
Satoshi Takahashi ◽  
Toyotaka Sato ◽  
Masaaki Shinagawa ◽  
Yukari Fukushima ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates

scholarly journals DNA gyrase mutations in quinolone-resistant clinical isolates of Neisseria gonorrhoeae

1995 ◽  
Vol 39 (2) ◽  
pp. 561-563 ◽  
Author(s):  
T. Deguchi ◽  
M. Yasuda ◽  
M. Asano ◽  
K. Tada ◽  
H. Iwata ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Dna Gyrase ◽  
Clinical Isolates
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