scholarly journals Preliminary evaluation of the ligase chain reaction for specific detection of Neisseria gonorrhoeae.

1992 ◽  
Vol 30 (12) ◽  
pp. 3089-3094 ◽  
Author(s):  
L Birkenmeyer ◽  
A S Armstrong
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Preliminary Evaluation ◽  
Specific Detection ◽  
Chain Reaction ◽  
Ligase Chain Reaction

Diagnosis of Neisseria gonorrhoeae infections in women by using the ligase chain reaction on patient-obtained vaginal swabs.

1997 ◽  
Vol 35 (8) ◽  
pp. 2129-2132 ◽  
Author(s):  
E W Hook ◽  
S F Ching ◽  
J Stephens ◽  
K F Hardy ◽  
K R Smith ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Vaginal Swabs

scholarly journals Pooling of Urine Samples for Screening for Neisseria gonorrhoeae by Ligase Chain Reaction: Accuracy and Application

1998 ◽  
Vol 36 (12) ◽  
pp. 3624-3628 ◽  
Author(s):  
Katherine A. Kacena ◽  
Sean B. Quinn ◽  
Suzanne C. Hartman ◽  
Thomas C. Quinn ◽  
Charlotte A. Gaydos
Keyword(s):  
High School Students ◽  
Neisseria Gonorrhoeae ◽  
Nurse Practitioners ◽  
Urine Samples ◽  
Chain Reaction ◽  
School Students ◽  
Ligase Chain Reaction ◽  
Military Recruits ◽  
Specific Culture ◽  
Urine Specimens

The accuracy of detection of genital Neisseria gonorrhoeae infection in pooled urine samples by ligase chain reaction (LCR) was examined in three populations. Firstly, urine specimens from 300 female military recruits (FMR) were tested by LCR individually and in pools of four and six. Secondly, 300 urine specimens from middle-school students (MSS) were tested individually by LCR, and then the processed specimens were stored frozen for subsequent testing in pools of 4 and 10. Thirdly, 600 frozen urine specimens from high-school students (HSS) were tested by using the LCR pooling algorithm, i.e., testing processed specimens in pools of four in one test unit dose, and retesting individual specimens from positive pools. Finally, the pooling algorithm results were compared to culture results for a subset of 344 students from the original 600 HSS from whom cervical or urethral samples were taken at the discretion of the school nurse practitioners. Compared to individual testing of specimens by LCR in the FMR population, the pooling-by-four algorithm was 100% sensitive (5 of 5) and 100% pool specific (70 of 70), and the pool-by-six algorithm was 100% sensitive (5 of 5) and 100% pool specific (45 of 45). In the MSS population, the pool-by-4 algorithm was 95.8% sensitive (23 of 24) and 100% (52 of 52) pool specific, and the pool-by-10 algorithm was 95.8% sensitive (23 of 24) and 100% (17 of 17) pool specific. In the subset of 344 HSS from whom endocervical or urethral specimens were collected for culture, 31 were positive by LCR in urine and 26 were positive by culture. After results discrepant between culture and LCR were adjudicated by a confirmatory LCR test, the pooling algorithm was 93.8% (30 of 32) sensitive and 99.7% (311 of 312) specific. Culture from these 344 HSS was 81.3% (26 of 32) sensitive. The pooling algorithm reduced the cost of the N. gonorrhoeae LCR assay by 60% compared to individual testing of the HSS specimens and was both sensitive and specific.

Ligase chain reaction-based electrochemical biosensor for the ultrasensitive and specific detection of single nucleotide polymorphisms

2019 ◽  
Vol 43 (36) ◽  
pp. 14327-14335 ◽  
Author(s):  
Wancun Zhang ◽  
Fang Hu ◽  
Xianwei Zhang ◽  
Wei Meng ◽  
Yaodong Zhang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Nucleotide Polymorphisms ◽  
Specific Detection ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Ligase Chain Reaction ◽  
Amplification Strategy

In this study, a sensitive electrochemical biosensor for universally, robustly, specifically, and sensitively detecting SNPs was developed by using LCR as a signal amplification strategy.

scholarly journals Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabs.

1995 ◽  
Vol 33 (12) ◽  
pp. 3111-3114 ◽  
Author(s):  
S Ching ◽  
H Lee ◽  
E W Hook ◽  
M R Jacobs ◽  
J Zenilman
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Chain Reaction ◽  
Ligase Chain Reaction

scholarly journals Evaluation of the Abbott LCx Ligase Chain Reaction Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine and Genital Swab Specimens from a Sexually Transmitted Disease Clinic Population

1998 ◽  
Vol 36 (6) ◽  
pp. 1630-1633 ◽  
Author(s):  
Karen C. Carroll ◽  
William E. Aldeen ◽  
Michael Morrison ◽  
Roberta Anderson ◽  
Deborah Lee ◽  
...  
Keyword(s):  
Data Analysis ◽  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Positive Result ◽  
Transmitted Disease ◽  
Urine Samples ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Pilin Gene ◽  
Urine Specimens

The Abbott LCx ligase chain reaction (LCR) assay for the simultaneous detection of Chlamydia trachomatis andNeisseria gonorrhoeae was evaluated by using swab and urine specimens from 562 patients. C. trachomatis results by LCR were compared to those by the Gen-Probe PACE 2 assay, whereas N. gonorrhoeae results by LCR were compared to those by culture. The Gen-Probe and LCR assays were performed according to the manufacturers’ instructions. Gram-negative diplococci growing on modified Thayer-Martin medium were confirmed as N. gonorrhoeae by the GonoGen II assay. Supplemental data analysis was performed by major outer membrane protein PCR for C. trachomatis and probes for pilin gene detection for N. gonorrhoeae. A true-positive result for each pathogen was defined as a positive result for all three or two of three assays. Overall agreement among the six assays was 94.8%. C. trachomatis prevalence was 16.2%; N. gonorrhoeae prevalence was 5.5%. The overall sensitivity and specificity, respectively, for each test (after supplemental data analysis) were as follows: for C. trachomatis, Gen-Probe, 65.9 and 100%; LCR on urine, 90.1 and 100%; LCR on swab specimens, 96.7 and 100%; and for N. gonorrhoeae, culture, 80.6 and 100%; LCR on urine, 93.5 and 99.8%; and LCR on swab specimens, 96.8 and 100%. For women, theN. gonorrhoeae culture was very insensitive compared to its performance in men (58.3 versus 94.7%, respectively). ForC. trachomatis, the Gen-Probe assay’s sensitivity was lower for men than for women (62.3 versus 71.1%, respectively). The sensitivity for C. trachomatis detection by LCR on urethral and cervical swab specimens was 96.2 and 97.4% for men and women, respectively. For men, swab results were slightly better than urine results for both pathogens (sensitivity for C. trachomatis in swab and urine specimens, 96.2 and 92.5%, respectively; sensitivity for N. gonorrhoeae in swab and urine specimens, 100 and 94.7%, respectively), while for women, cervical swabs were superior in sensitivity to urine samples for detecting C. trachomatis (swab, 97.4%; urine, 81.6%) and equivalent for N. gonorrhoeae (swab, 92.3%; urine, 91.6%). The LCx LCR appears to be both sensitive and specific for the detection of C. trachomatis andN. gonorrhoeae when performed on urine or genital swab samples. Swab samples had better sensitivity than urine samples for the detection of both pathogens.

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