scholarly journals Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus.

1993 ◽  
Vol 31 (3) ◽  
pp. 717-719 ◽  
Author(s):  
F Condorelli ◽  
T Ziegler
1994 ◽  
Vol 49 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Francesca Condorelli ◽  
Guido Scalia ◽  
Aldo Stivala ◽  
Rita Gallo ◽  
Anna Marino ◽  
...  

Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.


2009 ◽  
Vol 200 (7) ◽  
pp. 1031-1038 ◽  
Author(s):  
Nitya Nair ◽  
William J. Moss ◽  
Susana Scott ◽  
Nanthalile Mugala ◽  
Zaza M. Ndhlovu ◽  
...  

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.


2002 ◽  
Vol 40 (1) ◽  
pp. 111-116 ◽  
Author(s):  
M. del Mar Mosquera ◽  
F. de Ory ◽  
M. Moreno ◽  
J. E. Echevarria

2001 ◽  
Vol 39 (1) ◽  
pp. 170-174 ◽  
Author(s):  
M. B. Isa ◽  
L. Martinez ◽  
M. Giordano ◽  
M. Zapata ◽  
C. Passeggi ◽  
...  

2016 ◽  
Vol 54 (7) ◽  
pp. 1720-1725 ◽  
Author(s):  
Elise Bouthry ◽  
Milena Furione ◽  
Daniela Huzly ◽  
Adaeze Ogee-Nwankwo ◽  
LiJuan Hao ◽  
...  

Immunity to rubella virus (RV) is commonly determined by measuring specific immunoglobulin G (RV IgG). However, RV IgG results and their interpretation may vary, depending on the immunoassay, even though most commercial immunoassays (CIAs) have been calibrated against an international standard and results are reported in international units per milliliter. A panel of 322 sera collected from pregnant women that tested negative or equivocal for RV IgG in a prior test (routine screening) was selected. This panel was tested with two reference tests, immunoblotting (IB) and neutralization (Nt), and with 8 CIAs widely used in Europe. IB and Nt gave concordant results on 267/322 (82.9%) sera. Of these, 85 (26.4%) sera were negative and 182 (56.5%) sera were positive for both tests. All 85 IB/Nt-negative samples were classified as negative with all CIAs. Of the 182 IB/Nt-positive samples, 25.3 to 61.5% were classified as equivocal and 6 to 64.8% were classified as positive with the CIAs. Wide variations in titers in international units per milliliter were observed. In our series, more than half of the women considered susceptible to RV based on CIA results tested positive for RV antibodies by IB/Nt. Our data suggest that (i) sensitivity of CIAs could be increased by considering equivocal results as positive and (ii) the definition of immunity to RV as the 10-IU/ml usual cutoff as well as the use of quantitative results for clinical decisions may warrant reconsideration. A better standardization of CIAs for RV IgG determination is needed.


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