Detection of Human Herpesvirus 6 DNA in Serum by a Microplate PCR-Hybridization Assay

PCR was performed on DNA extracts derived from clinical serum samples submitted for human herpesvirus 6 (HHV-6) serological examination. To detect amplified HHV-6 products, a hybridization-based microtiter plate assay (PCR ELISA; Boehringer Mannheim) was used. The assay system was found to be rapid, specific, and sensitive. Approximately three copies of a plasmid-based HHV-6 sequence could be detected, and no cross amplification was observed with HHV-7 genomic DNA. There was no correlation found between HHV-6 DNA detection and serological status in clinical serum samples from individuals more than 2 years old. On the other hand, in serum samples from infants less than 2 years old, a high rate of detection of HHV-6 DNA was observed in those who lacked immunoglobulin G and M antibodies to HHV-6 (55%). In this regard, PCR of serum DNA extracts may be used as a sensitive indicator of active HHV-6 infection in infants prior to their seroconversion.

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Seroprevalence of Antibodies against Human Herpesvirus 6 in the Quebec City Area

Canadian Journal of Infectious Diseases ◽

10.1155/1992/837029 ◽

1992 ◽

Vol 3 (4) ◽

pp. 179-184 ◽

Cited By ~ 1

Author(s):

Louise Deschênes ◽

Jean R Joly ◽

Michel Couillard ◽

Gilles Richer

Keyword(s):

Cell Line ◽

Antibody Titre ◽

Geometric Mean ◽

Human Herpesvirus ◽

Quebec City ◽

Age Group ◽

Human Herpesvirus 6 ◽

Serum Samples ◽

City Area ◽

Herpesvirus 6

Seroprevalence of antibodies against human herpesvirus 6 was determined in a sample of 303 randomly selected individuals from the Quebec City area. The influence of different variables on antibody litres was also evaluated. Human herpesvirus 6 was grown in the HSB-2 cell line, and antibody litres were measured by indirect immunofluorescence. Serum samples were collected from 177 females and 126 males ranging in age from two months to 88 years. Ninety-nine per cent (300 of 303) of this population had an antibody titre of at least 1:10, whereas 75% had a titre of at least 1:80. Women had a higher geometric mean litre than men (P=0.06). This difference between sexes varied according to age and became statistically significant in subjects older than 20 years of age (P=0.04). It was found that this difference was attributable to higher antibody litres in women in the 15 to 40 year age group who had previously had children.

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Association of Active Human Herpesvirus-6, -7 and Parvovirus B19 Infection with Clinical Outcomes in Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

Advances in Virology ◽

10.1155/2012/205085 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 28

Author(s):

Svetlana Chapenko ◽

Angelika Krumina ◽

Inara Logina ◽

Santa Rasa ◽

Maksims Chistjakovs ◽

...

Keyword(s):

Viral Infection ◽

Proinflammatory Cytokines ◽

Parvovirus B19 ◽

Human Herpesvirus ◽

High Rate ◽

Simultaneous Increase ◽

Concurrent Infection ◽

Human Herpesvirus 6 ◽

Herpesvirus 6 ◽

Healthy Persons

Frequency of active human herpesvirus-6, -7 (HHV-6, HHV-7) and parvovirus B19 (B19) infection/coinfection and its association with clinical course of ME/CFS was evaluated. 108 ME/CFS patients and 90 practically healthy persons were enrolled in the study. Viral genomic sequences were detected by PCR, virus-specific antibodies and cytokine levels—by ELISA, HHV-6 variants—by restriction analysis. Active viral infection including concurrent infection was found in 64.8% (70/108) of patients and in 13.3% (12/90) of practically healthy persons. Increase in peripheral blood leukocyte DNA HHV-6 load as well as in proinflammatory cytokines' levels was detected in patients during active viral infection. Definite relationship was observed between active betaherpesvirus infection and subfebrility, lymphadenopathy and malaise after exertion, and between active B19 infection and multijoint pain. Neuropsychological disturbances were detected in all patients. The manifestation of symptoms was of more frequent occurrence in patients with concurrent infection. The high rate of active HHV-6, HHV-7 and B19 infection/coinfection with the simultaneous increase in plasma proinflammatory cytokines' level as well as the association between active viral infection and distinctive types of clinical symptoms shows necessity of simultaneous study of these viral infections for identification of possible subsets of ME/CFS.

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Association of human herpesvirus 6 infection with exanthem subitum in Belem, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651995000600003 ◽

1995 ◽

Vol 37 (6) ◽

pp. 489-492 ◽

Cited By ~ 4

Author(s):

R. B. de Freitas ◽

A. C. Linhares ◽

C. S. Oliveira ◽

R. H. P. Gusmão ◽

M. I. S. Linhares

Keyword(s):

Lymph Node ◽

Cervical Lymph Node ◽

Human Herpesvirus ◽

Human Herpesvirus 6 ◽

Antibody Assay ◽

Serum Samples ◽

Exanthem Subitum ◽

Lymph Node Swelling ◽

Herpesvirus 6 ◽

Immunofluorescence Antibody

Recent human herpesvirus 6 (HHV-6) infection was detected in cases of exanthem subitum (ES) involving four children, aged 10 to 24 months, between April and August 1994, in Belém, Brazil. By using the indirect immunofluorescence antibody assay (IFA), significant increases (at least eight times) in antibody concentrations were noted from the acute to the convalescent serum samples, with titers ranging from <1:10/1:80 to <1:10/1:640 (patients 3 and 2, respectively). All children had high fever (over 39ºC) for three days, followed by generalized, maculo-papular skin rash. A physical examination of the children also revealed concomitant, cervical lymph node swelling and tonsillar pharyngitis in two of them.

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