Reverse Transcriptase PCR Amplification ofRickettsia typhi from Infected Mammalian Cells and Insect Vectors

Journal of Clinical Microbiology ◽

10.1128/jcm.36.6.1793-1794.1998 ◽

1998 ◽

Vol 36 (6) ◽

pp. 1793-1794 ◽

Author(s):

James A. Higgins ◽

Suzana Radulovic ◽

Bruce H. Noden ◽

Jill M. Troyer ◽

Abdu F. Azad

Keyword(s):

Reverse Transcriptase ◽

Vero Cell ◽

Mammalian Cells ◽

Pcr Amplification ◽

Insect Vectors ◽

Pcr Assay ◽

Rickettsia Typhi ◽

Reverse Transcriptase Pcr ◽

Infected Vero Cell ◽

High Concentrations

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

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Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care

Journal of Clinical Microbiology ◽

10.1128/jcm.01712-07 ◽

2007 ◽

Vol 45 (11) ◽

pp. 3498-3505 ◽

Author(s):

S. E. Letant ◽

J. I. Ortiz ◽

L. F. Bentley Tammero ◽

J. M. Birch ◽

R. W. Derlet ◽

...

Keyword(s):

Reverse Transcriptase ◽

Point Of Care ◽

Respiratory Pathogens ◽

Pcr Assay ◽

Reverse Transcriptase Pcr ◽

Viral Respiratory

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Diagnostic accuracy of an allele-specific reverse transcriptase-PCR assay targeting the H275Y oseltamivir resistant mutation in 2009 pandemic influenza A/H1N1 virus

Journal of Clinical Virology ◽

10.1016/j.jcv.2010.06.019 ◽

2010 ◽

Vol 49 (1) ◽

pp. 21-25 ◽

Author(s):

C. Renaud ◽

J. Kuypers ◽

L. Corey

Keyword(s):

Diagnostic Accuracy ◽

Reverse Transcriptase ◽

Pandemic Influenza ◽

Influenza A ◽

Pcr Assay ◽

Reverse Transcriptase Pcr ◽

Influenza A H1n1 ◽

Allele Specific ◽

Resistant Mutation

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A Multiplex Reverse Transcriptase-PCR Assay for the Genotyping of Avian Infectious Bronchitis Viruses

Avian Diseases Digest ◽

10.1637/9257.1 ◽

2010 ◽

Vol 5 (1) ◽

pp. e20-e20

Author(s):

Hui-Wen Chen ◽

Ching-Ho Wang

Keyword(s):

Reverse Transcriptase ◽

Pcr Assay ◽

Infectious Bronchitis ◽

Reverse Transcriptase Pcr

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Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification

Protocols for Nucleic Acid Analysis by Nonradioactive Probes ◽

10.1385/1-59745-229-7:143 ◽

2007 ◽

pp. 143-152

Author(s):

María Rosa Ponce ◽

Víctor Quesada ◽

Andrea Hricová ◽

José Luis Micol

Keyword(s):

Gene Expression ◽

Reverse Transcriptase ◽

Pcr Amplification ◽

Reverse Transcriptase Pcr

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The prognostic value of a reverse transcriptase-PCR assay of sentinel lymph node biopsy for patients with cutaneous melanoma

Melanoma Research ◽

10.1097/cmr.0b013e32834dcfdf ◽

2012 ◽

Vol 22 (1) ◽

pp. 38-44 ◽

Author(s):

Takamichi Ito ◽

Yoichi Moroi ◽

Junna Oba ◽

Takeshi Nakahara ◽

Satoshi Takeuchi ◽

...

Keyword(s):

Sentinel Lymph Node ◽

Sentinel Lymph Node Biopsy ◽

Reverse Transcriptase ◽

Cutaneous Melanoma ◽

Prognostic Value ◽

Lymph Node Biopsy ◽

Pcr Assay ◽

Reverse Transcriptase Pcr ◽

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Validation of a Real-Time Reverse Transcriptase–PCR Assay for the Detection of H7 Avian Influenza Virus

Avian Diseases Digest ◽

10.1637/9215-891109-digest.1 ◽

2010 ◽

Vol 5 (s1) ◽

pp. e148-e149

Author(s):

Janice Pedersen ◽

Mary Lea Killian ◽

Nichole Hines ◽

Dennis Senne ◽

Brundaban Panigrahy ◽

...

Keyword(s):

Influenza Virus ◽

Avian Influenza ◽

Reverse Transcriptase ◽

Avian Influenza Virus ◽

Real Time ◽

Pcr Assay ◽

Reverse Transcriptase Pcr

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Detection of Wuchereria bancrofti L3 Larvae in Mosquitoes: A Reverse Transcriptase PCR Assay Evaluating Infection and Infectivity

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0000602 ◽

2010 ◽

Vol 4 (2) ◽

pp. e602 ◽

Author(s):

Sandra J. Laney ◽

Reda M. R. Ramzy ◽

Hanan H. Helmy ◽

Hoda A. Farid ◽

Ameen A. Ashour ◽

...

Keyword(s):

Reverse Transcriptase ◽

Wuchereria Bancrofti ◽

Pcr Assay ◽

Reverse Transcriptase Pcr

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Comparison of a Real-Time Reverse Transcriptase PCR Assay and a Culture Technique for Quantitative Assessment of Viral Load in Children Naturally Infected with Respiratory Syncytial Virus

Journal of Clinical Microbiology ◽

10.1128/jcm.43.5.2356-2362.2005 ◽

2005 ◽

Vol 43 (5) ◽

pp. 2356-2362 ◽

Author(s):

S. M. Perkins ◽

D. L. Webb ◽

S. A. Torrance ◽

C. El Saleeby ◽

L. M. Harrison ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Reverse Transcriptase ◽

Real Time ◽

Quantitative Assessment ◽

Culture Technique ◽

Pcr Assay ◽

Reverse Transcriptase Pcr ◽

Syncytial Virus

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A Sensitive, Specific, and Cost-Effective Multiplex Reverse Transcriptase-PCR Assay for the Detection of Seven Common Respiratory Viruses in Respiratory Samples

Journal of Molecular Diagnostics ◽

10.1016/s1525-1578(10)60500-4 ◽

2004 ◽

Vol 6 (2) ◽

pp. 125-131 ◽

Author(s):

Melanie W. Syrmis ◽

David M. Whiley ◽

Marion Thomas ◽

Ian M. Mackay ◽

Jeanette Williamson ◽

...

Keyword(s):

Reverse Transcriptase ◽

Cost Effective ◽

Respiratory Viruses ◽

Pcr Assay ◽

Reverse Transcriptase Pcr

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Comparison of storage methods for reverse-transcriptase PCR amplification of rotavirus RNA from gorilla (Gorilla g. gorilla) fecal samples

Journal of Virological Methods ◽

10.1016/j.jviromet.2003.10.003 ◽

2004 ◽

Vol 116 (1) ◽

pp. 11-17 ◽

Author(s):

Christopher A Whittier ◽

William Horne ◽

Barrett Slenning ◽

Michael Loomis ◽

Michael K Stoskopf

Keyword(s):

Reverse Transcriptase ◽

Pcr Amplification ◽

Gorilla Gorilla ◽

Fecal Samples ◽

Reverse Transcriptase Pcr ◽

Storage Methods

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