Bacteriological and Serological Findings in a Further Case of Transfusion-Mediated Yersinia enterocolitica Sepsis

A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin M (IgM), IgA, and IgG antibodies against Yersinia outer proteins (YopM, -H, -D, and -E) were found. Since the donor remembered a short-lasting, mild diarrhea 14 days prior to blood donation, a transient attack of Yersinia enteritis may be associated with a longer than expected period of asymptomatic bacteremia that causes contamination of donor blood. Serological screening for IgM antibodies against Yersinia outer proteins might offer a way to reduce the risk of transfusion-associated Y. enterocolitica sepsis.

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Seroprevalence of Anti-SARS-CoV-2 IgG and IgM among Adults over 65 Years Old in the South of Italy

Diagnostics ◽

10.3390/diagnostics11030483 ◽

2021 ◽

Vol 11 (3) ◽

pp. 483

Author(s):

Immacolata Polvere ◽

Alfredina Parrella ◽

Giovanna Casamassa ◽

Silvia D’Andrea ◽

Annamaria Tizzano ◽

...

Keyword(s):

Antibody Response ◽

Immunoglobulin M ◽

Molecular Testing ◽

Elisa Assay ◽

The South ◽

Serum Samples ◽

Serological Screening ◽

Rna Detection ◽

In Vitro Diagnostic

SARS-CoV-2 is a zoonotic betacoronavirus associated with worldwide transmission of COVID-19 disease. By the beginning of March, WHO reported about 113,820,000 confirmed cases including more than 2,527,000 deaths all over the world. However, the true extent of virus circulation or its real infection/fatality ratio is not well-estimated due to the huge portion of asymptomatic infections. In this observational study, we have estimated the prevalence of specific immunoglobulin M and G directed towards SARS-CoV-2 antigen in a cohort of 1383 adult volunteers aged over 65 years old, living in the district of Benevento, in the South of Italy. Serological screening was carried out on capillary blood in September 2020, seven months after pandemic outbreak in Italy, to evaluate virus circulation and antibody response among elderly adults, in which severe symptoms due to viral infection are more common. The overall seroprevalence of anti-SARS-CoV-2 antibodies was 4.70% (CI 3.70%–5.95%) with no statistically significant differences between sexes. Among these, 69.69% (CI 55.61%–77.80%) tested positive to IgM, 23.08% (CI 14.51%–34.64%) to IgG and 9.23% (CI 4.30%–18.71%) was positive for both. All patients that were positive to IgM underwent molecular testing through RT-qPCR on oral-rhino pharyngeal swabs and only one specimen was positive for SARS-CoV-2 RNA detection. Instead, the presence of IgG from screened volunteers was confirmed by re-testing serum samples using both an ELISA assay validated for in vitro diagnostic use (IVD) and a recently published synthetic peptide-based ELISA assay. In conclusion, our report suggests that (1) early restrictions were successful in limiting COVID-19 diffusion in the district of Benevento; (2) rapid serological analysis is an ideal testing for both determining real seroprevalence and massive screening, whereas detection of viral RNA remains a gold standard for identification of infected patients; (3) even among people without COVID-19 related symptoms, the antibody response against SARS-CoV-2 antigens has individual features.

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Hospital-Based Donor Recruitment and Predonation Serologic Testing for COVID-19 Convalescent Plasma

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqaa268 ◽

2021 ◽

Author(s):

Joanna Balcerek ◽

Evelin Trejo ◽

Kendall Levine ◽

Paul Couey ◽

Zoe V Kornberg ◽

...

Keyword(s):

Blood Donation ◽

High Titer ◽

Igg Antibodies ◽

Serologic Testing ◽

Wide Range ◽

The Us ◽

Polymerase Chain ◽

Igg Level ◽

Donor Recruitment ◽

Selection Of

Abstract Objectives Serologic testing for antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in potential donors of coronavirus disease 2019 (COVID-19) convalescent plasma (CCP) may not be performed until after blood donation. A hospital-based recruitment program for CCP may be an efficient way to identify potential donors prospectively Methods Patients who recovered from known or suspected COVID-19 were identified and recruited through medical record searches and public appeals in March and April 2020. Participants were screened with a modified donor history questionnaire and, if eligible, were asked for consent and tested for SARS-CoV-2 antibodies (IgG and IgM). Participants positive for SARS-CoV-2 IgG were referred for CCP collection. Results Of 179 patients screened, 128 completed serologic testing and 89 were referred for CCP donation. IgG antibodies to SARS-CoV-2 were detected in 23 of 51 participants with suspected COVID-19 and 66 of 77 participants with self-reported COVID-19 confirmed by polymerase chain reaction (PCR). The anti–SARS-CoV-2 IgG level met the US Food and Drug Administration criteria for “high-titer” CCP in 39% of participants confirmed by PCR, as measured by the Ortho VITROS IgG assay. A wide range of SARS-CoV-2 IgG levels were observed. Conclusions A hospital-based CCP donor recruitment program can prospectively identify potential CCP donors. Variability in SARS-CoV-2 IgG levels has implications for the selection of CCP units for transfusion.

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Aortoabdominal aneurysm infected by Yersinia enterocolitica serotype O:9

Journal of Infection ◽

10.1016/s0163-4453(97)93536-2 ◽

1997 ◽

Vol 35 (3) ◽

pp. 314-315 ◽

Cited By ~ 7

Author(s):

B. La Scola ◽

D. Musso ◽

A. Carta ◽

P. Piquet ◽

J.-P. Casalta

Keyword(s):

Yersinia Enterocolitica ◽

Serotype O

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Functional Mapping of YadA- and Ail-Mediated Binding of Human Factor H to Yersinia enterocolitica Serotype O:3

Infection and Immunity ◽

10.1128/iai.00314-08 ◽

2008 ◽

Vol 76 (11) ◽

pp. 5016-5027 ◽

Cited By ~ 45

Author(s):

Marta Biedzka-Sarek ◽

Saara Salmenlinna ◽

Markus Gruber ◽

Andrei N. Lupas ◽

Seppo Meri ◽

...

Keyword(s):

Yersinia Enterocolitica ◽

Alternative Pathway ◽

Outer Membrane Proteins ◽

Factor H ◽

Complement Alternative Pathway ◽

Human Host ◽

Serotype O ◽

Complement Resistance ◽

Sense And Respond ◽

Active Complement

ABSTRACT Yersinia enterocolitica is an enteric pathogen that exploits diverse means to survive in the human host. Upon Y. enterocolitica entry into the human host, bacteria sense and respond to variety of signals, one of which is the temperature. Temperature in particular has a profound impact on Y. enterocolitica gene expression, as most of its virulence factors are expressed exclusively at 37°C. These include two outer membrane proteins, YadA and Ail, that function as adhesins and complement resistance (CR) factors. Both YadA and Ail bind the functionally active complement alternative pathway regulator factor H (FH). In this study, we characterized regions on both proteins involved in CR and the interaction with FH. Twenty-eight mutants having short (7 to 41 amino acids) internal deletions within the neck and stalk of YadA and two complement-sensitive site-directed Ail mutants were constructed to map the CR and FH binding regions of YadA and Ail. Functional analysis of the YadA mutants revealed that the stalk of YadA is required for both CR and FH binding and that FH appears to target several conformational and discontinuous sites of the YadA stalk. On the other hand, the complement-sensitive Ail mutants were not affected in FH binding. Our results also suggested that Ail- and YadA-mediated CR does not depend solely on FH binding.

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Yersinia enterocolitica isolates from humans in California, 1968-1975

Journal of Clinical Microbiology ◽

10.1128/jcm.4.2.137-144.1976 ◽

1976 ◽

Vol 4 (2) ◽

pp. 137-144

Author(s):

M L Bissett

Keyword(s):

Yersinia Enterocolitica ◽

Antimicrobial Susceptibility ◽

Breast Abscess ◽

Biochemical Tests ◽

California Department ◽

Biochemical Characteristics ◽

Serotype O ◽

Department Of Health ◽

Antimicrobial Susceptibility Tests ◽

Susceptibility Tests

This paper reports on the serological and biochemical characteristics of 24 human isolates of Yersinia enterocolitica submitted to the California Department of Health from 1968 through 1975. Nine different serotypes were represented. The majority of strains were serotype O:8 (six strains) and serotype O:5 (five strains). Sources of the isolates included feces (12 cases), blood (3), sputum or throat (3), bile or bowel drainage (2), wounds (2), breast abscess (1), and skin abscess (1). Clinical histories indicated a number of different syndromes. Underlying medical conditions existed in 13 cases. Results of selected biochemical tests and antimicrobial susceptibility tests on the strains indicated grouping compatible with the O serotypes of the organisms.

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