scholarly journals Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test

2002 ◽  
Vol 40 (11) ◽  
pp. 4161-4165 ◽  
Author(s):  
A. Ito ◽  
N. Xiao ◽  
M. Liance ◽  
M. O. Sato ◽  
Y. Sako ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Immunosorbent Assay ◽  
Alveolar Echinococcosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blind Test

scholarly journals Determination of Vegetal Proteins in Milk Powder by Enzyme-Linked Immunosorbent Assay: Interlaboratory Study

2002 ◽  
Vol 85 (6) ◽  
pp. 1390-1397 ◽  
Author(s):  
Lourdes Sánchez ◽  
María D Pérez ◽  
Pilar Puyol ◽  
Miguel Calvo ◽  
Gary Brett ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Collaborative Study ◽  
Milk Powder ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Proteins ◽  
Blind Test ◽  
Relative Standard ◽  
Standard Deviations ◽  
Phosphate Buffered Saline

Abstract Eight laboratories participated in a collaborative study to evaluate an enzyme-linked immunosorbent assay (ELISA) to determine soy, pea, and wheat proteins in pasteurized or ultra-high temperature (UHT) milk powders. To perform this assay, polyclonal antibodies for soy, pea, and wheat proteins were obtained from rabbit sera. Collaborators received calibration standards composed of milk powder containing 0–8% (w/w) vegetal protein in total protein and blind test samples containing approximately 1, 2, and 5% (w/w) vegetal protein. An indirect competitive ELISA was performed with a kit prepared by a participating laboratory; the kit contained plates coated with soy, pea, or wheat proteins, the corresponding specific antisera, enzyme-labeled second antibody, and substrate solution. Test samples and calibrants were extracted with phosphate-buffered saline, pH 7.4, containing 0.05% Tween and assayed with the ELISA kits. The degree of adulteration was affected by the type of heat treatment applied to the samples. The estimated percentage of vegetal protein addition was close to the theoretical value for pasteurized samples but much lower for UHT samples. For pasteurized samples, intralaboratory relative standard deviations ranged from 5 to 22% and interlaboratory relative standard deviations ranged from 14 to 34%.

scholarly journals Differential Diagnosis of Flavivirus Infections in Horses Using Viral Envelope Protein Domain III Antigens in Enzyme-Linked Immunosorbent Assay

2017 ◽  
Vol 17 (12) ◽  
pp. 825-835 ◽  
Author(s):  
Thisun B.H. Piyasena ◽  
Yin X. Setoh ◽  
Jody Hobson-Peters ◽  
Natalie A. Prow ◽  
Helle Bielefeldt-Ohmann ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Immunosorbent Assay ◽  
Envelope Protein ◽  
Viral Envelope ◽  
Protein Domain ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Envelope Protein ◽  
Domain Iii

scholarly journals Synthesis of the Carbohydrate Moiety of Glycoproteins from the Parasite Echinococcus granulosus and Their Antigenicity against Human Sera

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5652
Author(s):  
Noriyasu Hada ◽  
Tokio Morita ◽  
Takashi Ueda ◽  
Kazuki Masuda ◽  
Hiromi Nakane ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Immunosorbent Assay ◽  
Cystic Echinococcosis ◽  
Alveolar Echinococcosis ◽  
Total Yield ◽  
Carbohydrate Moiety ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Sera

Stereocontrolled syntheses of biotin-labeled oligosaccharide portions containing the carbohydrate moiety of glycoprotein from Echinococcus granulosus have been accomplished. Trisaccharide Galβ1-3Galβ1-3GalNAcα1-R (A), tetrasaccharide Galα1-4Galβ1-3Galβ1-3GalNAcα1-R (B), and pentasaccharide Galα1-4Galβ1-3Galβ1-3Galβ1-3GalNAcα1-R (C), (R = biotinylated probe) were synthesized by stepwise condensation and/or block synthesis by the use of 5-(methoxycarbonyl)pentyl 2-azido-4,6-O-benzylidene-2-deoxy-α-d-galactopyranoside as a common glycosyl acceptor. The synthesis of the tetrasaccharide and the pentasaccharide was improved from the viewpoint of reducing the number of synthetic steps and increasing the total yield by changing from stepwise condensation to block synthesis. Moreover, hexasaccharide E, which contains the oligosaccharide sequence which occurs in E. granulosus, was synthesized from trisaccharide D. We examined the antigenicity of these five oligosaccharides by an enzyme-linked immunosorbent assay (ELISA). Although compounds of C–E did not exhibit antigenicity against cystic echinococcosis (CE) patient sera, compounds B, D, and E showed good serodiagnostic potential for alveolar echinococcosis (AE).

scholarly journals Detection of Echinococcus Multilocularis Infection in a Colony of Cynomolgus Monkeys (Macaca Fascicularis) using Serology and Ultrasonography

2005 ◽  
Vol 17 (2) ◽  
pp. 183-186 ◽  
Author(s):  
Patrick Rehmann ◽  
Andrea Gröne ◽  
Bruno Gottstein ◽  
Jörg Völlm ◽  
Heinz Sager ◽  
...  
Keyword(s):  
Bile Duct ◽  
Immunosorbent Assay ◽  
Echinococcus Multilocularis ◽  
Macaca Fascicularis ◽  
Alveolar Echinococcosis ◽  
Postmortem Examination ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cynomolgus Monkeys ◽  
Serological Examination

Five animals in a colony of cynomolgus monkeys ( Macaca fascicularis) died or were euthanatized because of alveolar echinococcosis, during a period of 5 years. The remainder of the colony was screened for possible infection with Echinococcus multilocularis, using serology and ultrasonography. A total of 46 animals out of a group of 55 were examined. The presence of anti-Em2 antibodies analyzed with enzyme-linked immunosorbent assay was demonstrated in 3 monkeys. In 2 of these 3 monkeys, multilocular structures compatible with metacestodal cysts in the liver were identified, using ultrasonography. The presence of alveolar echinococcosis was subsequently confirmed at postmortem examination in 1 animal. The other animals are still alive. Two other monkeys were negative in the serological examination but had cystic structures in the liver, which were identified as bile duct cysts at postmortem examination in 1 animal. The other monkey is still alive. These findings suggest that serology for antibodies against the Em2 antigen may represent a useful method in identifying animals that might be infected with E. multilocularis and are therefore at risk of developing fatal alveolar echinococcosis.

Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis

2006 ◽  
Vol 80 (4) ◽  
pp. 387-391 ◽  
Author(s):  
K. Yamano ◽  
N. Hada ◽  
T. Yamamura ◽  
T. Takeda ◽  
H. Honma ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Immunosorbent Assay ◽  
Echinococcus Multilocularis ◽  
Cystic Echinococcosis ◽  
Alveolar Echinococcosis ◽  
Carbohydrate Antigen ◽  
Single Component ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Specific Reactions

AbstractIn the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids fromEchinococcus multiloculariswere examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1-ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.

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