scholarly journals Phenotypic and Molecular Characterization of Erythromycin Resistance in Four Isolates of Streptococcus-Like Gram-Positive Cocci Causing Bacteremia

2004 ◽  
Vol 42 (7) ◽  
pp. 3303-3305 ◽  
Author(s):  
P. C. Y. Woo ◽  
A. P. C. To ◽  
S. K. P. Lau ◽  
A. M. Y. Fung ◽  
K.-y. Yuen
1999 ◽  
Vol 181 (20) ◽  
pp. 6347-6353 ◽  
Author(s):  
Richard van Kranenburg ◽  
Harmjan R. Vos ◽  
Iris I. van Swam ◽  
Michiel Kleerebezem ◽  
Willem M. de Vos

ABSTRACT Sixteen exopolysaccharide (EPS)-producing Lactococcus lactis strains were analyzed for the chemical compositions of their EPSs and the locations, sequences, and organization of theeps genes involved in EPS biosynthesis. This allowed the grouping of these strains into three major groups, representatives of which were studied in detail. Previously, we have characterized theeps gene cluster of strain NIZO B40 (group I) and determined the function of three of its glycosyltransferase (GTF) genes. Fragments of the eps gene clusters of strains NIZO B35 (group II) and NIZO B891 (group III) were cloned, and these encoded the NIZO B35 priming galactosyltransferase, the NIZO B891 priming glucosyltransferase, and the NIZO B891 galactosyltransferase involved in the second step of repeating-unit synthesis. The NIZO B40 priming glucosyltransferase gene epsD was replaced with an erythromycin resistance gene, and this resulted in loss of EPS production. This epsD deletion was complemented with priming GTF genes from gram-positive organisms with known function and substrate specificity. Although no EPS production was found with priming galactosyltransferase genes from L. lactis orStreptococcus thermophilus, complementation with priming glucosyltransferase genes involved in L. lactis EPS andStreptococcus pneumoniae capsule biosynthesis could completely restore or even increase EPS production in L. lactis.


2007 ◽  
Vol 11 (4) ◽  
pp. 329-336 ◽  
Author(s):  
Jinnethe Reyes ◽  
Marylin Hidalgo ◽  
Lorena Díaz ◽  
Sandra Rincón ◽  
Jaime Moreno ◽  
...  

2010 ◽  
Vol 42 (10) ◽  
pp. 1857-1863 ◽  
Author(s):  
Ramesh C. Patra ◽  
Seidu Malik ◽  
Michael Beer ◽  
Mallavarapu Megharaj ◽  
Ravi Naidu

Plasmid ◽  
1994 ◽  
Vol 31 (1) ◽  
pp. 60-71 ◽  
Author(s):  
Gerald W. Tannock ◽  
John B. Luchansky ◽  
Lynette Miller ◽  
Hugh Connell ◽  
Soren Thode-Andersen ◽  
...  

1997 ◽  
Vol 179 (17) ◽  
pp. 5632-5635 ◽  
Author(s):  
M J Pucci ◽  
J A Thanassi ◽  
L F Discotto ◽  
R E Kessler ◽  
T J Dougherty

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