scholarly journals Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory †

2017 ◽  
Vol 18 (2) ◽  
Author(s):  
Kristen Bowey-Dellinger ◽  
Luke Dixon ◽  
Kristin Ackerman ◽  
Cynthia Vigueira ◽  
Yewseok K. Suh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Undergraduate Biology ◽  
Biology Laboratory

scholarly journals Introducing Mammalian Cell Colony Formation in the Undergraduate Biology Laboratory

2020 ◽  
Author(s):  
Ashley Robinson ◽  
Mackenzie Crow ◽  
Austin Kratz ◽  
Taylor Ritts ◽  
Yewseok K. Suh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Hela Cells ◽  
Colony Formation ◽  
Cytotoxic Agents ◽  
Undergraduate Biology ◽  
Clonogenic Assays ◽  
Laboratory Exercise ◽  
Biology Laboratory ◽  
Cell Colony

Clonogenic assays are a simple and robust method that allow researchers to characterize mammalian cell line features, including the ability of a single cell to grow into a colony. We have used this assay as a tool in the undergraduate biology laboratory, exposing students to a more specialized form of mammalian cell culture and helping them refine scientific research skills and knowledge. In this article, we share an easy and undergraduate-friendly method of using HeLa cells to carry out clonogenic assays. The methods described include the introduction of different treatments to assess their effect in HeLa cell colony formation. In this laboratory exercise, undergraduate students utilize different cell culture techniques such as growing, harvesting, counting, diluting, staining, and imaging cells. Clonogenic assay, Cytotoxic agents, HeLa cells, Mammalian cell colony formation, undergraduate laboratory, Open Inquiry-Based Curriculum

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

1999 ◽  
Vol 34 (2) ◽  
pp. 159-165 ◽  
Author(s):  
J. Feuser ◽  
M. Halfar ◽  
D. Lütkemeyer ◽  
N. Ameskamp ◽  
M.-R. Kula ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Culture Broth ◽  
Expanded Bed Adsorption ◽  
Expanded Bed

Mammalian cell culture: engineering principles and scale-up

1983 ◽  
Vol 1 (4) ◽  
pp. 102-108 ◽  
Author(s):  
M.W. Glacken ◽  
R.J. Fleischaker ◽  
A.J. Sinskey
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Scale Up ◽  
Cell Culture Engineering

Hydrogen peroxide production is affected by oxygen levels in mammalian cell culture

2017 ◽  
Vol 493 (1) ◽  
pp. 246-251 ◽  
Author(s):  
Lucas A. Maddalena ◽  
Shehab M. Selim ◽  
Joao Fonseca ◽  
Holt Messner ◽  
Shannon McGowan ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Hydrogen Peroxide Production ◽  
Oxygen Levels

Expression of recombinant calf prochymosin in mammalian cell culture

1991 ◽  
Vol 20 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Meelis Kolmer ◽  
Tõnis Örd ◽  
Ismo Ulmanen
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture

Oxygen transfer rates in a mammalian cell culture bioreactor equipped with a cell-lift impeller

1990 ◽  
Vol 35 (1) ◽  
pp. 43-49 ◽  
Author(s):  
M. Johnson ◽  
G. André ◽  
C. Chavarie ◽  
J. Archambault
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Oxygen Transfer ◽  
Mammalian Cell Culture ◽  
Transfer Rates ◽  
A Cell
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