scholarly journals Role of Untranslated Regions of the Hemagglutinin-Neuraminidase Gene in Replication and Pathogenicity of Newcastle Disease Virus

2009 ◽  
Vol 83 (11) ◽  
pp. 5943-5946 ◽  
Author(s):  
Yongqi Yan ◽  
Subrat N. Rout ◽  
Shin-Hee Kim ◽  
Siba K. Samal

ABSTRACT To determine the role of untranslated regions (UTRs) in replication and pathogenesis of Newcastle disease virus (NDV), we generated recombinant viruses with deletions in 5′ and 3′ UTRs of the HN mRNA. Deletion of any HN UTR did not noticeably affect in vitro replication of these viruses. However, complete deletion of the 5′ UTR of the HN gene decreased the HN mRNA levels and HN protein contents in virus particles, resulting in attenuation of the virus in chickens. This indicates that the 5′ UTR of HN mRNA plays an important role in replication and pathogenicity of NDV in vivo.

2021 ◽  
Vol 100 (5) ◽  
pp. 101067
Author(s):  
Ana Paula Del Vesco ◽  
Hyun Jun Jang ◽  
Melissa S. Monson ◽  
Susan J. Lamont

2004 ◽  
Vol 17 (4) ◽  
pp. 543-547 ◽  
Author(s):  
I. K. Park ◽  
H. L. Jiang ◽  
C. H. Yun ◽  
Y. J. Choi ◽  
S. J. Kim ◽  
...  

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1249
Author(s):  
Archana Jadhav ◽  
Lele Zhao ◽  
Alice Ledda ◽  
Weiwei Liu ◽  
Chan Ding ◽  
...  

The expression of accessory non-structural proteins V and W in Newcastle disease virus (NDV) infections depends on RNA editing. These proteins are derived from frameshifts of the sequence coding for the P protein via co-transcriptional insertion of one or two guanines in the mRNA. However, a larger number of guanines can be inserted with lower frequencies. We analysed data from deep RNA sequencing of samples from in vitro and in vivo NDV infections to uncover the patterns of mRNA editing in NDV. The distribution of insertions is well described by a simple Markov model of polymerase stuttering, providing strong quantitative confirmation of the molecular process hypothesised by Kolakofsky and collaborators three decades ago. Our results suggest that the probability that the NDV polymerase would stutter is about 0.45 initially, and 0.3 for further subsequent insertions. The latter probability is approximately independent of the number of previous insertions, the host cell, and viral strain. However, in LaSota infections, we also observe deviations from the predicted V/W ratio of about 3:1 according to this model, which could be attributed to deviations from this stuttering model or to further mechanisms downregulating the abundance of W protein.


Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 962 ◽  
Author(s):  
Panrao Liu ◽  
Yuncong Yin ◽  
Yabin Gong ◽  
Xusheng Qiu ◽  
Yingjie Sun ◽  
...  

Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.


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