scholarly journals Novel MicroRNAs (miRNAs) Encoded by Herpesvirus of Turkeys: Evidence of miRNA Evolution by Duplication

2009 ◽  
Vol 83 (13) ◽  
pp. 6969-6973 ◽  
Author(s):  
Yongxiu Yao ◽  
Yuguang Zhao ◽  
Lorraine P. Smith ◽  
Michael Watson ◽  
Venugopal Nair
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Phylogenetic Relationships ◽  
Viral Genome ◽  
Herpesvirus Of Turkeys ◽  
Positional Conservation ◽  
Disease Virus Type ◽  
Mirna Evolution ◽  
Close Sequence

ABSTRACT Herpesviruses account for 134 out of the 140 virus-encoded microRNAs (miRNAs) known today. Here we report the identification of 11 novel miRNAs encoded by herpesvirus of turkey (HVT), a virus used as a live vaccine in poultry against the highly oncogenic Marek's disease virus type 1. Ten of these miRNAs were clustered together within the repeat long region of the viral genome, demonstrating some degree of positional conservation with other mardiviruses. Close sequence and phylogenetic relationships of some miRNAs in this cluster indicate evolution by duplication. HVT miRNAs represent the first example of virus-encoded miRNAs that show evolution by duplication.

Differentiation of Oncogenic and Nononcogenic Strains of Marek's Disease Virus Type 1 by Using Polymerase Chain Reaction DNA Amplification

1992 ◽  
Vol 36 (3) ◽  
pp. 637 ◽  
Author(s):  
Geng-Sheng Zhu ◽  
Tomoko Ojima ◽  
Takashi Hironaka ◽  
Takeshi Ihara ◽  
Noriko Mizukoshi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Virus Type ◽  
Dna Amplification ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Chain Reaction ◽  
Disease Virus Type ◽  
Polymerase Chain

scholarly journals Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

2000 ◽  
Vol 74 (7) ◽  
pp. 3217-3226 ◽  
Author(s):  
Kengo Sonoda ◽  
Masashi Sakaguchi ◽  
Hiroshi Okamura ◽  
Kenji Yokogawa ◽  
Eiji Tokunaga ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Type ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Maternal Antibodies ◽  
F Protein ◽  
Polyvalent Vaccine ◽  
Disease Virus Type ◽  
Commercial Chickens

ABSTRACT An earlier report (M. Sakaguchi et al., Vaccine 16:472–479, 1998) showed that recombinant Marek's disease virus type 1 (rMDV1) expressing the fusion (F) protein of Newcastle disease virus (NDV-F) under the control of the simian virus 40 late promoter [rMDV1-US10L(F)] protected specific pathogen-free chickens from NDV challenge, but not commercial chickens with maternal antibodies against NDV and MDV1. In the present study, we constructed an improved polyvalent vaccine based on MDV1 against MDV and NDV in commercial chickens with maternal antibodies. The study can be summarized as follows. (i) We constructed rMDV1 expressing NDV-F under the control of the MDV1 glycoprotein B (gB) promoter [rMDV1-US10P(F)]. (ii) Much less NDV-F protein was expressed in cells infected with rMDV1-US10P(F) than in those infected with rMDV1-US10L(F). (iii) The antibody response against NDV-F and MDV1 antigens of commercial chickens vaccinated with rMDV1-US10P(F) was much stronger and faster than with rMDV1-US10L(F), and a high level of antibody against NDV-F persisted for over 80 weeks postvaccination. (iv) rMDV1-US10P(F) was readily reisolated from the vaccinated chickens, and the recovered viruses were found to express NDV-F. (v) Vaccination of commercial chickens having maternal antibodies to rMDV1-US10P(F) completely protected them from NDV challenge. (vi) rMDV1-US10P(F) offered the same degree of protection against very virulent MDV1 as the parental MDV1 and commercial vaccines. These results indicate that rMDV1-US10P(F) is an effective and stable polyvalent vaccine against both Marek's and Newcastle diseases even in the presence of maternal antibodies.

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