Expression of Extremely Low Levels of Thymidine Kinase from an Acyclovir-Resistant Herpes Simplex Virus Mutant Supports Reactivation from Latently Infected Mouse Trigeminal Ganglia
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ABSTRACT A single-cytosine-deletion in the herpes simplex virus gene encoding thymidine kinase (TK) was previously found in an acyclovir-resistant clinical isolate. A laboratory strain engineered to carry this mutation did not generate sufficient TK activity for detection by plaque autoradiography, which detected 0.25% wild-type activity. However, a drug sensitivity assay suggested that extremely low levels of TK are generated by this virus. The virus was estimated to express 0.09% of wild-type TK activity via a ribosomal frameshift 24 nucleotides upstream of the mutation. Remarkably, this appeared to be sufficient active TK to support a low level of reactivation from latently infected mouse trigeminal ganglia.
1994 ◽
Vol 75
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pp. 2469-2474
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2006 ◽
Vol 87
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pp. 3495-3502
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1984 ◽
Vol 83
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pp. 344-346
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2004 ◽
Vol 78
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pp. 520-523
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1998 ◽
Vol 72
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pp. 7115-7124
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1981 ◽
Vol 34
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pp. 987-992
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1990 ◽
Vol 71
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pp. 125-132
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