scholarly journals Cross-Reactive anti-Nucleocapsid Protein Immunity against Crimean-Congo Hemorrhagic Fever Virus and Hazara Virus in Multiple Species

2021 ◽  
Author(s):  
Merve Kalkan-Yazıcı ◽  
Elif Karaaslan ◽  
Nesibe Selma Çetin ◽  
Sevde Hasanoğlu ◽  
Filiz Güney ◽  
...  
Keyword(s):  
Surrogate Model ◽  
Nucleocapsid Protein ◽  
Hemorrhagic Fever ◽  
Cross Reactivity ◽  
Polyclonal Antiserum ◽  
The Other ◽  
Crimean Congo Hemorrhagic Fever ◽  
Humoral Immune ◽  
Hemorrhagic Fever Virus ◽  
Multiple Species

The World Health Organization estimates that there may be three billion people at risk of infection by Crimean-Congo Hemorrhagic Fever Virus (CCHFV), a highly lethal, emerging orthonairovirus carried by ticks. On the other hand, the closely related Hazara virus (HAZV), a member of the same serogroup, has not been reported as a pathogen for humans. Given the structural and phylogenetic similarities between these two viruses, we evaluated the immunological similarities of the nucleocapsid protein (NP) of these two viruses in multiple species. Strong antigenic similarities were demonstrated in anti-NP humoral immune responses against HAZV and CCHFV in multiple species using convalescent human CCHF sera, rabbit and mouse polyclonal antiserum raised against CCHFV, and mouse polyclonal antiserum against CCHFV-NP in enzyme immunoassays. We also report a convincing cross-reactivity between NPs in Western blots using HAZV-infected cell lysate as antigen and inactivated CCHFV and CCHFV-NP-immunized mice sera. These results suggest that NPs of HAZV and CCHFV share significant similarities in humoral responses across species and underline the potential utility of HAZV as a surrogate model for CCHFV. IMPORTANCE CCHFV and HAZV, members of the Nairoviridae family, are transmitted to mammals by tick bites. CCHFV is considered to be a severe threat to public health and causes hemorrhagic diseases with a high mortality rate, and there are neither preventative nor therapeutic medications against CCHFV disease. HAZV, on the other hand, is not a pathogen to humans and can be studied under BSL-2 conditions. The antigenic relationship between these viruses is of interest for vaccines and for preventative investigations. Here, we demonstrate cross-reactivity in anti-NP humoral immune response between NPs of HAZV and CCHFV in multiple species. These results underline the utility of HAZV as a surrogate model to study CCHFV infection.

scholarly journals Similarities in Antiviral Humoral Immune Response to Nucleocapsid Proteins of Hazara and Crimean–Congo Hemorrhagic Fever Virus

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 72
Author(s):  
Merve Kalkan-Yazıcı ◽  
Mehmet Ziya Doymaz
Keyword(s):  
Humoral Response ◽  
Hemorrhagic Fever ◽  
Cross Reactivity ◽  
Fever Virus ◽  
Nucleocapsid Proteins ◽  
Virus Like Particles ◽  
Crimean Congo Hemorrhagic Fever ◽  
Humoral Immune ◽  
Hemorrhagic Fever Virus ◽  
Transmission Electron

Hazara virus (HAZV), a tick-borne agent of the nairoviruses, is closely related to Crimean–Congo hemorrhagic fever virus (CCHFV). Hazara virus has not been reported as a pathogen for humans and can be studied under BSL-2 conditions, whereas CCHFV causes severe hemorrhagic diseases, with up to 30% mortality rate in humans, and requires BSL-4 facilities to be handled. Serologic and phylogenetic similarities between the two viruses would therefore be an interesting area of research. In this study, we evaluated the immunological similarities between these two viruses using nucleocapsid protein as a model. Here, we evaluated cross-reactivity between CCHFV and HAZV rNP, which forms virus-like particles when expressed in Pichia pastoris. In Western blot assays using CCHFV-infected human and immunized mice and rabbit sera, cross-reactions were detected between the nucleoproteins of both viruses. Virus-like particles were visualized by transmission electron microscopy (TEM) and monitored by dynamic light scattering (DLS). These results suggest that nucleocapsid proteins of HAZV and CCHFV share similarities regarding the antiviral humoral response in both species.

scholarly journals Crimean-Congo hemorrhagic fever virus nucleocapsid protein harbors distinct RNA-binding sites in the stalk and head domains

2019 ◽  
Vol 294 (13) ◽  
pp. 5023-5037 ◽  
Author(s):  
Subbiah Jeeva ◽  
Sheema Mir ◽  
Adrain Velasquez ◽  
Jacquelyn Ragan ◽  
Aljona Leka ◽  
...  
Keyword(s):  
Binding Sites ◽  
Nucleocapsid Protein ◽  
Rna Binding ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Rna Binding Sites

scholarly journals Crimean-Congo hemorrhagic fever virus nucleocapsid protein has dual RNA binding modes

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184935 ◽  
Author(s):  
Subbiah Jeeva ◽  
Sean Pador ◽  
Brittany Voss ◽  
Safder Saieed Ganaie ◽  
Mohammad Ayoub Mir
Keyword(s):  
Nucleocapsid Protein ◽  
Rna Binding ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Binding Modes ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

scholarly journals Structure, Function, and Evolution of the Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein

2012 ◽  
Vol 86 (20) ◽  
pp. 10914-10923 ◽  
Author(s):  
S. D. Carter ◽  
R. Surtees ◽  
C. T. Walter ◽  
A. Ariza ◽  
E. Bergeron ◽  
...  
Keyword(s):  
Structure Function ◽  
Nucleocapsid Protein ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

scholarly journals Adjuvant Potential of CD24 on Immunogenicity and Lethal Challenge Protection of a DNA vector Expressing Nucleocapsid Protein of Crimean Congo Hemorrhagic Fever Virus

2018 ◽  
Author(s):  
Touraj Aligholipour Farzani ◽  
Alireza Hanifehnezhad ◽  
Katalin Foldes ◽  
Koray Ergunay ◽  
Erkan Yilmaz ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Vaccine Development ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Foreign Gene ◽  
Lethal Challenge ◽  
Crimean Congo Hemorrhagic Fever ◽  
N Protein ◽  
Hemorrhagic Fever Virus ◽  
Dna Vector

Crimean Congo hemorrhagic fever virus (CCHFV) is the causative agent of a globally-spread tick-borne zoonotic infection with an eminent risk of fatal human disease. Imminent public health threat posed by disseminated virus activity and lack of an approved therapeutic make CCHFV an urgent target for vaccine development. We described the construction of a DNA vector expressing nucleocapsid protein (N) of CCHFV (pV-N13) and investigated its potential to stimulate cytokine and total/specific antibody responses in BALB/c and challenge experiment in IFNAR-/- mice. Due to lack of sufficient antibody stimulation towards N protein, we have selected CD24 protein as a potential adjuvant which has proliferative effect on B and T cells. Overall, our N expressing construct when administered solely or in combination with pCD24 vector elicited significant cellular and humoral responses in BALB/c, despite variations in particular cytokines and total antibodies. However, the stimulated antibodies produced due to expression of N protein have shown no neutralizing ability in VNA. Furthermore, challenge experiments were revealed protection potential of N expressing construct in IFNAR -/- mice model. In conclusion, we have shown that CD24 has prominent effect as a genetic adjuvant when co-delivers with a synergic foreign gene expressing vector. Besides, targeting of S segment of CCHFV can be considered as a practical way in developing vaccine against this virus due to its ability to induce immune response which leads to protection in challenge assays in IFN-gamma defective mice models.

scholarly journals Mapping of the interaction domains of the Crimean–Congo hemorrhagic fever virus nucleocapsid protein

2015 ◽  
Vol 96 (3) ◽  
pp. 524-537 ◽  
Author(s):  
Jesica M. Levingston Macleod ◽  
Hannah Marmor ◽  
Adolfo García-Sastre ◽  
Natalia Frias-Staheli
Keyword(s):  
Nucleocapsid Protein ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Interaction Domains
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