scholarly journals Cell Surface Antigens Associated with Murine Leukemia Virus: Definition of the GL and GT Antigenic Systems

1973 ◽  
Vol 12 (5) ◽  
pp. 1104-1117 ◽  
Author(s):  
Robert C. Nowinski ◽  
Elizabeth D. Peters
Keyword(s):  
Cell Surface ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Definition Of ◽  
Murine Leukemia

scholarly journals Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma.

1977 ◽  
Vol 146 (3) ◽  
pp. 720-734 ◽  
Author(s):  
A B DeLeo ◽  
H Shiku ◽  
T Takahashi ◽  
M John ◽  
L J Old
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Cultured Fibroblasts ◽  
Chemically Induced ◽  
Sarcoma Cells ◽  
Murine Leukemia

As background for a serological definition of the unique antigens of chemically induced sarcomas, we have typed a series of fibroblast and sarcoma cell lines of BALB/c and C57BL/6 origin by cytoxicity and absorption tests for murine leukemia virus (MuLV)-related cell surface antigens and known alloantigens. 7 of the 17 cultured lines expressed the range of cell surface antigens associated with MuLV (GIX, GCSA, gp70, p30), and this was invariably associated with MuLV production. In nonproducer lines of C57BL/6 (but not BALB/c) origin, a MuLV-gp70-like molecule was found on the surface of fibroblasts and sarcoma cells. The alloantigenic phenotype of these MuLV+ and MuLV- cell lines was H-2D+, H-2K+, Thy-1.2+ or -, PC.1+ or -, Lyt-1.2-, Lyt-2.2-, Ia.7-, and TL.2-. A unique antigen was defined on the BALB/c ascites sarcoma Meth A with antisera prepared in BALB/c or (BALB/c X C57BL/6)F1 mice. Tissue culture lines derived from this tumor were MuLV-, which facilitated serological study of the antigen. Absorption analysis indicated that the antigen was restricted to Meth A; it could not be detected in normal or fetal BALB/c tissue MuLV+ or MuLV- fibroblast lines, 12 syngeneic or allogeneic sarcomas, or normal lymphoid cells from 13 different inbred mouse strains.

Correlation between various cell-surface antigens induced by murine leukemia virus in the rat: Serological analysis

1973 ◽  
Vol 11 (3) ◽  
pp. 575-585 ◽  
Author(s):  
Noboru Kuzumaki ◽  
Noritoshi Takeichi ◽  
Fujiro Sendo ◽  
Takao Kodama ◽  
Hiroshi Kobayashi
Keyword(s):  
Cell Surface ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Serological Analysis ◽  
Cell Surface Antigens ◽  
Murine Leukemia

scholarly journals Specificity studies on cytolytic T lymphocytes directed against murine leukemia virus-induced tumors. Analysis of monoclonal cytolytic T lymphocytes.

1982 ◽  
Vol 155 (4) ◽  
pp. 1050-1062 ◽  
Author(s):  
F Plata
Keyword(s):  
T Lymphocytes ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Target Cells ◽  
Cytolytic T Lymphocytes ◽  
Tumor Target ◽  
Induced Tumors ◽  
Definition Of ◽  
Leukemia Viruses ◽  
Murine Leukemia

The specificities of cloned cytolytic T lymphocytes (CTL) were studied for the analysis of CTL populations generated against murine leukemia viruses (MuLV) in H-2 congenic BALB/c (H-2d) and BALB.B (H-2b) mice. In particular, CTL generated in response to tumors induced by Gross MuLV and Friend MuLV were studied; these tumors expressed virus-induced antigens that do not cross-react and that can be distinguished from each other. The systematic study of 92 CTL clones clearly indicated that MuLV-immune CTL were highly heterogeneous with respect to both the intensities of target cell lysis that they mediated and to their specificity of recognition of MuLV-induced tumor target cells. Various categories of CTL clones were identified, ranging from CTL clones tht were tightly H-2 restricted and specific for the immunizing tumor to CTL clones that displayed no discernible patterns of specificity and that attacked a large number of different target cells. In addition, the surface markers of these cloned CTL were defined, and the best conditions for their prolonged maintenance in culture were determined. The present data indicate that future efforts in the definition of target antigens recognized by tumor-specific CTL should be performed with monoclonal lymphocytes.

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