scholarly journals The RNA polymerase II 15-kilodalton subunit is essential for viability in Drosophila melanogaster.

1992 ◽  
Vol 12 (3) ◽  
pp. 928-935 ◽  
Author(s):  
D A Harrison ◽  
M A Mortin ◽  
V G Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Product ◽  
Metal Binding ◽  
Developmental Stages ◽  
Larval Instar ◽  
A Subunit ◽  
Transplantation Experiments ◽  
Early Larval Development

A small, divergently transcribed gene is located 500 bp upstream of the suppressor of Hairy-wing locus of Drosophila melanogaster. Sequencing of a full-length cDNA clone of the predominant 850-nucleotide transcript reveals that this gene encodes a 15,100-Da protein with high homology to a subunit of RNA polymerase II. The RpII15 protein is 46% identical to the RPB9 protein of Saccharomyces cerevisiae, one of the smallest subunits of RNA polymerase II from that species. Among those identical residues are four pairs of cysteines whose spacing is suggestive of two metal-binding "finger" domains. The gene is expressed at all developmental stages and in all tissues. Two deletions within the RpII15 gene are multiphasic lethal deletions, with accumulation of dead animals commencing at the second larval instar. Ovary transplantation experiments indicate that survival of mutant animals to this stage is due to the persistence of maternal gene product throughout embryogenesis and early larval development. The RpII15 gene product is thus necessary for viability of D. melanogaster.

The RNA polymerase II 15-kilodalton subunit is essential for viability in Drosophila melanogaster

1992 ◽  
Vol 12 (3) ◽  
pp. 928-935
Author(s):  
D A Harrison ◽  
M A Mortin ◽  
V G Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Product ◽  
Metal Binding ◽  
Developmental Stages ◽  
Larval Instar ◽  
A Subunit ◽  
Transplantation Experiments ◽  
Early Larval Development

A small, divergently transcribed gene is located 500 bp upstream of the suppressor of Hairy-wing locus of Drosophila melanogaster. Sequencing of a full-length cDNA clone of the predominant 850-nucleotide transcript reveals that this gene encodes a 15,100-Da protein with high homology to a subunit of RNA polymerase II. The RpII15 protein is 46% identical to the RPB9 protein of Saccharomyces cerevisiae, one of the smallest subunits of RNA polymerase II from that species. Among those identical residues are four pairs of cysteines whose spacing is suggestive of two metal-binding "finger" domains. The gene is expressed at all developmental stages and in all tissues. Two deletions within the RpII15 gene are multiphasic lethal deletions, with accumulation of dead animals commencing at the second larval instar. Ovary transplantation experiments indicate that survival of mutant animals to this stage is due to the persistence of maternal gene product throughout embryogenesis and early larval development. The RpII15 gene product is thus necessary for viability of D. melanogaster.

scholarly journals Tyrosine Phosphorylation of RNA Polymerase II Carboxyl-terminal Domain by the Abl-related Gene Product

1997 ◽  
Vol 272 (30) ◽  
pp. 18905-18909 ◽  
Author(s):  
R. Baskaran ◽  
Gary G. Chiang ◽  
Tami Mysliwiec ◽  
Gary D. Kruh ◽  
Jean Y. J. Wang
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Tyrosine Phosphorylation ◽  
Gene Product ◽  
Related Gene ◽  
Terminal Domain ◽  
Carboxyl Terminal Domain ◽  
Carboxyl Terminal

scholarly journals RPII15 codes for the M r 15,000 subunit 9 of Drosophila melanogaster RNA polymerase II

FEBS Letters ◽  
1993 ◽  
Vol 335 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Zhihong Liu ◽  
Roland E. Kontermann ◽  
Rüdiger A. Schulze ◽  
Gabriele Petersen ◽  
Ekkehard K.F. Bautz
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Subunit 9

scholarly journals Protein-DNA cross-linking reveals dramatic variation in RNA polymerase II density on different histone repeats of Drosophila melanogaster.

1987 ◽  
Vol 7 (9) ◽  
pp. 3341-3344 ◽  
Author(s):  
D S Gilmour ◽  
J T Lis
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Topoisomerase I ◽  
Transcriptional Activity ◽  
Base Pairs ◽  
Nontranscribed Spacer ◽  
Short Repeat ◽  
Dramatic Variation

In Drosophila melanogaster the five histone genes are within a 5-kilobase region which is repeated 100 times at a single chromosomal site. These 5-kilobase repeats are of two distinct classes, short and long, that differ by approximately 200 base pairs of DNA in the spacer region between the H1 and H3 genes. Since the mRNA-homologous regions of the repeats are highly conserved, one cannot examine differential expression of the repeats by classical hybridization methods. In this study, we assessed their transcriptional activity by measuring in vivo the relative amounts of RNA polymerase II that were cross-linked by UV irradiation to the two different histone repeats. The RNA polymerase II density on the long repeat in Schneider line 2 cells was strikingly lower (10-fold) than the density on the short repeat. The magnitude of this difference cannot be accounted for by reduced transcription of only one or two genes of the repeat. The density of topoisomerase I, an indicator of transcriptional activity, was also much higher on the short repeat than on the long repeat of line 2 cells. In contrast, the RNA polymerase II density was slightly higher on the long repeat than on the short repeat in a second cell line, KcH. The major difference between active (KcH) and inactive (S2) long repeats resides in the H1-H3 nontranscribed spacer. This portion of the spacer may contain a component necessary for expression that can act over a moderate distance and affect multiple genes of the repeat.

scholarly journals The Cockayne syndrome group A gene encodes a WD repeat protein that interacts with CSB protein and a subunit of RNA polymerase II TFIIH

Cell ◽  
1995 ◽  
Vol 82 (4) ◽  
pp. 555-564 ◽  
Author(s):  
Karla A. Henning ◽  
Lei Li ◽  
Narayan Iyer ◽  
Lisa D. McDaniel ◽  
Michael S. Reagan ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cockayne Syndrome ◽  
Repeat Protein ◽  
Group A ◽  
A Subunit ◽  
Wd Repeat
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