Growth factor-induced delayed early response genes

1992 ◽  
Vol 12 (9) ◽  
pp. 3919-3929
Author(s):  
A Lanahan ◽  
J B Williams ◽  
L K Sanders ◽  
D Nathans
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Early Response ◽  
Integral Membrane Protein ◽  
Early Gene ◽  
Immediate Early ◽  
Human Macrophage ◽  
Cellular Genes ◽  
Gene Response ◽  
Early Response Genes

Growth factors induce the sequential expression of cellular genes whose products are thought to mediate long-term responses to the growth factors. In mouse 3T3 fibroblastic cells, the first genes to be expressed (immediate-early genes) are activated within minutes after the addition of platelet-derived growth factor, fibroblast growth factor, or serum. By cDNA cloning, we have identified genes that are activated after a delay of a few hours and several hours prior to serum-induced DNA replication. Activation of these delayed early response genes requires new protein synthesis, presumably the synthesis of immediate-early transcription factors described previously. Partial or complete sequencing of 13 different delayed early cDNAs, representing about 40% of the 650 primary cDNA isolates, revealed that 8 were related to known gene sequences and 5 were not. Among the former are cDNAs encoding nonhistone chromosomal proteins [HMGI(Y) and HMGI-C], adenine phosphoribosyltransferase (APRT), a protein related to human macrophage migration inhibitory factor (MIF), a protein of the major intrinsic protein (MIP) family homologous to the integral membrane protein of human erythrocytes, and cyclin CYL1. In 3T3 cells, the delayed early gene response to growth factors appears to be at least as complex as the immediate-early gene response previously described.

scholarly journals Growth factor-induced delayed early response genes.

1992 ◽  
Vol 12 (9) ◽  
pp. 3919-3929 ◽  
Author(s):  
A Lanahan ◽  
J B Williams ◽  
L K Sanders ◽  
D Nathans
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Early Response ◽  
Integral Membrane Protein ◽  
Early Gene ◽  
Immediate Early ◽  
Human Macrophage ◽  
Cellular Genes ◽  
Gene Response ◽  
Early Response Genes

Growth factors induce the sequential expression of cellular genes whose products are thought to mediate long-term responses to the growth factors. In mouse 3T3 fibroblastic cells, the first genes to be expressed (immediate-early genes) are activated within minutes after the addition of platelet-derived growth factor, fibroblast growth factor, or serum. By cDNA cloning, we have identified genes that are activated after a delay of a few hours and several hours prior to serum-induced DNA replication. Activation of these delayed early response genes requires new protein synthesis, presumably the synthesis of immediate-early transcription factors described previously. Partial or complete sequencing of 13 different delayed early cDNAs, representing about 40% of the 650 primary cDNA isolates, revealed that 8 were related to known gene sequences and 5 were not. Among the former are cDNAs encoding nonhistone chromosomal proteins [HMGI(Y) and HMGI-C], adenine phosphoribosyltransferase (APRT), a protein related to human macrophage migration inhibitory factor (MIF), a protein of the major intrinsic protein (MIP) family homologous to the integral membrane protein of human erythrocytes, and cyclin CYL1. In 3T3 cells, the delayed early gene response to growth factors appears to be at least as complex as the immediate-early gene response previously described.

Promiscuous and Depolarization-Induced Immediate-Early Response Genes Are Induced by Mechanical Strain of Osteoblasts

2009 ◽  
Vol 24 (7) ◽  
pp. 1247-1262 ◽  
Author(s):  
Claus-Eric Ott ◽  
Sebastian Bauer ◽  
Thomas Manke ◽  
Susan Ahrens ◽  
Christian Rödelsperger ◽  
...  
Keyword(s):  
Mechanical Strain ◽  
Early Response ◽  
Immediate Early ◽  
Early Response Genes

scholarly journals A novel 7-nucleotide motif located in 3' untranslated sequences of the immediate-early gene set mediates platelet-derived growth factor induction of the JE gene.

1992 ◽  
Vol 12 (12) ◽  
pp. 5288-5300 ◽  
Author(s):  
R R Freter ◽  
J C Irminger ◽  
J A Porter ◽  
S D Jones ◽  
C D Stiles
Keyword(s):  
Growth Factor ◽  
Immediate Early Genes ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Platelet Derived Growth Factor ◽  
Immediate Early ◽  
Control Element ◽  
Gene Set ◽  
Cis Acting ◽  
Early Genes

A cohort of the serum and growth factor regulated immediate-early gene set is induced with slower kinetics than c-fos. Two of the first immediate-early genes characterized as such, c-myc and JE, are contained within this subset. cis-acting genomic elements mediating induction of the slower responding subset of immediate-early genes have never been characterized. Herein we characterize two widely separated genomic elements which are together essential for induction of the murine JE gene by platelet-derived growth factor, serum, interleukin-1, and double-stranded RNA. One of these elements is novel in several regards. It is a 7-mer, TTTTGTA, found in the proximal 3' sequences downstream of the JE stop codon. The 3' element is position dependent and orientation independent. It does not function in polyadenylation, splicing, or destabilization of the JE transcript. Copies of the 7-mer or its inverse are found at comparable 3' sites in 25 immediate-early genes that encode transcription factors or cytokines. Given its general occurrence, the 7-mer may be a required cis-acting control element mediating induction of the immediate-early gene set.

Pip92: a short-lived, growth factor-inducible protein in BALB/c 3T3 and PC12 cells

1990 ◽  
Vol 10 (12) ◽  
pp. 6769-6774
Author(s):  
C H Charles ◽  
J S Simske ◽  
T P O'Brien ◽  
L F Lau
Keyword(s):  
Amino Acids ◽  
Growth Factors ◽  
Growth Factor ◽  
Neuronal Differentiation ◽  
Cellular Responses ◽  
Early Gene ◽  
Extracellular Signals ◽  
Pheochromocytoma Cell ◽  
Inducible Protein ◽  
Proline Rich Protein

pip92 is a cellular immediate-early gene inducible by serum growth factors in fibroblasts. It is also induced in the rat pheochromocytoma cell line PC12 by agents that cause proliferation, neuronal differentiation, and membrane depolarization. We show that the pip92-encoded polypeptide is a proline-rich protein of 221 amino acids, has an extremely short half-life, and is localized in the cytoplasm. We hypothesize that Pip92 plays a role in mediating the cellular responses to a variety of extracellular signals.

Effect of a dominant inhibitory Ha-ras mutation on neuronal differentiation of PC12 cells

1990 ◽  
Vol 10 (10) ◽  
pp. 5324-5332
Author(s):  
J Szeberényi ◽  
H Cai ◽  
G M Cooper
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Neuronal Differentiation ◽  
Pc12 Cells ◽  
Morphological Differentiation ◽  
Early Response ◽  
Secondary Response ◽  
Response Gene ◽  
Early Response Gene ◽  
Early Response Genes

A dominant inhibitory mutation of Ha-ras which changes Ser-17 to Asn-17 in the gene product p21 [p21 (Asn-17)Ha-ras] has been used to investigate the role of ras in neuronal differentiation of PC12 cells. The growth of PC12 cells, in contrast to NIH 3T3 cells, was not inhibited by p21(Asn-17)Ha-ras expression. However, PC12 cells expressing the mutant Ha-ras protein showed a marked inhibition of morphological differentiation induced by nerve growth factor (NGF) or fibroblast growth factor (FGF). These cells, however, were still able to respond with neurite outgrowth to dibutyryl cyclic AMP and 12-O-tetradecanoylphorbol-13-acetate (TPA). Induction of early-response genes (fos, jun, and zif268) by NGF and FGF but not by TPA was also inhibited by high levels of p21(Asn-17)Ha-ras. However, lower levels of p21(Asn-17) expression were sufficient to block neuronal differentiation without inhibiting induction of these early-response genes. Induction of the secondary-response genes SCG10 and transin by NGF, like morphological differentiation, was inhibited by low levels of p21(Asn-17) whether or not induction of early-response genes was blocked. Therefore, although inhibition of ras function can inhibit early-response gene induction, this is not required to block morphological differentiation or secondary-response gene expression. These results suggest that ras proteins are involved in at least two different pathways of signal transduction from the NGF receptor, which can be distinguished by differential sensitivity to p21(Asn-17)Ha-ras. In addition, ras and protein kinase C can apparently induce early-response gene expression by independent pathways in PC12 cells.

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