scholarly journals Stimulation of Mitotic Recombination Events by High Levels of RNA Polymerase II Transcription in Yeast

2000 ◽  
Vol 20 (15) ◽  
pp. 5404-5414 ◽  
Author(s):  
Dean Saxe ◽  
Abhijit Datta ◽  
Sue Jinks-Robertson
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Conversion ◽  
Regulatory Protein ◽  
Mitotic Recombination ◽  
Gene Conversion Event ◽  
Conversion Event ◽  
The Impact ◽  
Protein Transcription ◽  
Rna Polymerase Ii Transcription

ABSTRACT The impact of high levels of RNA polymerase II transcription on mitotic recombination was examined using lys2 recombination substrates positioned on nonhomologous chromosomes. Substrates were used that could produce Lys+ recombinants by either a simple (noncrossover) gene conversion event or a crossover-associated recombination event, by only a simple gene conversion event, or by only a crossover event. Transcription of the lys2 substrates was regulated by the highly inducible GAL1-10 promoter or the low-level LYS2 promoter, with GAL1-10 promoter activity being controlled by the presence or absence of the Gal80p negative regulatory protein. Transcription was found to stimulate recombination in all assays used, but the level of stimulation varied depending on whether only one or both substrates were highly transcribed. In addition, there was an asymmetry in the types of recombination events observed when one substrate versus the other was highly transcribed. Finally, the lys2 substrates were positioned as direct repeats on the same chromosome and were found to exhibit a different recombinational response to high levels of transcription from that exhibited by the repeats on nonhomologous chromosomes. The relevance of these results to the mechanisms of transcription-associated recombination are discussed.

RAD52-INDEPENDENT MITOTIC GENE CONVERSION IN SACCHAROMYCES CEREVISIAE FREQUENTLY RESULTS IN CHROMOSOMAL LOSS

Genetics ◽  
1985 ◽  
Vol 111 (1) ◽  
pp. 7-22
Author(s):  
James E Haber ◽  
Mark Hearn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mitotic Recombination ◽  
Gene Conversion Event ◽  
Intragenic Recombination ◽  
Wild Type ◽  
Conversion Event ◽  
Chromosomal Loss ◽  
Mitotic Gene Conversion ◽  
Striking Effect

ABSTRACT We have examined spontaneous, interchromosomal mitotic recombination events between his4 alleles in both Rad+ and rad52 strains of Saccharomyces cerevisiae. In Rad+ strains, 74% of the His+ prototrophs resulted from gene conversion events without exchange of flanking markers. In diploids homozygous for the rad52-1 mutation, the frequency of His+ prototroph formation was less than 5% of the wild-type value, and more than 80% of the gene conversion events were accompanied by an exchange of flanking markers. Most of the rad52 intragenic recombination events arose by gene conversion accompanied by an exchange of flanking markers and not by a simple reciprocal exchange between the his4A and his4C alleles. There were also profound effects on the kinds of recombinant products that were recovered. The most striking effect was that RAD52-independent mitotic recombination frequently results in the loss of one of the two chromosomes participating in the gene conversion event.

scholarly journals Prenatal diagnosis of the fragile X syndrome: loss of mutation owing to a double recombinant or gene conversion event at the FMR1 locus.

1997 ◽  
Vol 34 (11) ◽  
pp. 924-926 ◽  
Author(s):  
M Losekoot ◽  
E Hoogendoorn ◽  
R Olmer ◽  
C C Jansen ◽  
J C Oosterwijk ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Gene Conversion ◽  
Fragile X Syndrome ◽  
Fragile X ◽  
Gene Conversion Event ◽  
Conversion Event ◽  
Or Gene ◽  
Double Recombinant

Non-coding-RNA regulators of RNA polymerase II transcription

2006 ◽  
Vol 7 (8) ◽  
pp. 612-616 ◽  
Author(s):  
James A. Goodrich ◽  
Jennifer F. Kugel
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Non Coding Rna ◽  
Rna Polymerase Ii Transcription

scholarly journals The Ras/PKA Signaling Pathway May Control RNA Polymerase II Elongation via the Spt4p/Spt5p Complex in Saccharomyces cerevisiae

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1059-1070
Author(s):  
Susie C Howard ◽  
Arelis Hester ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Elongation Factor ◽  
Ras Signaling ◽  
Elongation Step ◽  
Rna Polymerase Ii Transcription

Abstract The Ras signaling pathway in Saccharomyces cerevisiae controls cell growth via the cAMP-dependent protein kinase, PKA. Recent work has indicated that these effects on growth are due, in part, to the regulation of activities associated with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. However, the precise target of these Ras effects has remained unknown. This study suggests that Ras/PKA activity regulates the elongation step of the RNA polymerase II transcription process. Several lines of evidence indicate that Spt5p in the Spt4p/Spt5p elongation factor is the likely target of this control. First, the growth of spt4 and spt5 mutants was found to be very sensitive to changes in Ras/PKA signaling activity. Second, mutants with elevated levels of Ras activity shared a number of specific phenotypes with spt5 mutants and vice versa. Finally, Spt5p was efficiently phosphorylated by PKA in vitro. Altogether, the data suggest that the Ras/PKA pathway might be directly targeting a component of the elongating polymerase complex and that this regulation is important for the normal control of yeast cell growth. These data point out the interesting possibility that signal transduction pathways might directly influence the elongation step of RNA polymerase II transcription.

Nucleophosmin Interacts with HEXIM1 and Regulates RNA Polymerase II Transcription

2008 ◽  
Vol 378 (2) ◽  
pp. 302-317 ◽  
Author(s):  
Meera Gurumurthy ◽  
Chuan Hao Tan ◽  
Raymond Ng ◽  
Lisa Zeiger ◽  
Joanne Lau ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rna Polymerase Ii Transcription
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