scholarly journals Factors influencing the accumulation of tetraphenylphosphonium cation in HeLa cells.

1984 ◽  
Vol 4 (1) ◽  
pp. 199-202 ◽  
Author(s):  
R Hiller ◽  
A Schaefer ◽  
R Zibirre ◽  
H R Kaback ◽  
G Koch
Keyword(s):  
Cell Cycle ◽  
Amino Acids ◽  
Steady State ◽  
Hela Cells ◽  
Alkylating Agent ◽  
State Level ◽  
Steady State Level ◽  
Factors Influencing ◽  
Rapid Accumulation ◽  
External K

Exposure of HeLa cells to tetraphenylphosphonium cation (TPP+) results in a rapid accumulation intracellularly, and a steady-state level is reached within 10 min. Accumulation of [3H]TPP+ in HeLa cells is reduced under the following conditions: (i) after preincubation of cells in buffered saline or in medium containing two- to fourfold higher concentrations of amino acids, (ii) exposure to the alkylating agent L-1-tosylamido-2-phenyl-ethylchloromethyl ketone, (iii) ouabain-mediated inhibition of the Na+, K+ ATPase, and (iv) high external K+ concentrations. In contrast, addition of serum increases the uptake of TPP+. In synchronized cells, intracellular levels of TPP+ differ at various stages of cell cycle and are lowest in mitosis.

Factors influencing the accumulation of tetraphenylphosphonium cation in HeLa cells

1984 ◽  
Vol 4 (1) ◽  
pp. 199-202
Author(s):  
R Hiller ◽  
A Schaefer ◽  
R Zibirre ◽  
H R Kaback ◽  
G Koch
Keyword(s):  
Cell Cycle ◽  
Amino Acids ◽  
Steady State ◽  
Hela Cells ◽  
Alkylating Agent ◽  
State Level ◽  
Steady State Level ◽  
Factors Influencing ◽  
Rapid Accumulation ◽  
External K

Exposure of HeLa cells to tetraphenylphosphonium cation (TPP+) results in a rapid accumulation intracellularly, and a steady-state level is reached within 10 min. Accumulation of [3H]TPP+ in HeLa cells is reduced under the following conditions: (i) after preincubation of cells in buffered saline or in medium containing two- to fourfold higher concentrations of amino acids, (ii) exposure to the alkylating agent L-1-tosylamido-2-phenyl-ethylchloromethyl ketone, (iii) ouabain-mediated inhibition of the Na+, K+ ATPase, and (iv) high external K+ concentrations. In contrast, addition of serum increases the uptake of TPP+. In synchronized cells, intracellular levels of TPP+ differ at various stages of cell cycle and are lowest in mitosis.

scholarly journals Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase.

1985 ◽  
Vol 5 (1) ◽  
pp. 226-235 ◽  
Author(s):  
T A Peterson ◽  
L Prakash ◽  
S Prakash ◽  
M A Osley ◽  
S I Reed
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Steady State ◽  
Uv Light ◽  
State Level ◽  
S Phase ◽  
Dna Ligase ◽  
Mrna Levels ◽  
Steady State Level ◽  
High Level

We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S phase. This high level of CDC9 mRNA then decays with an apparent half-life of ca. 20 min and remains at a low basal level throughout the rest of the cell cycle. The accumulation of CDC9 mRNA in late G1 is dependent upon the completion of start but not the CDC7 and CDC8 functions. Exposure of cells to UV light elicits an eightfold increase in DNA ligase mRNA levels.

Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase

1985 ◽  
Vol 5 (1) ◽  
pp. 226-235
Author(s):  
T A Peterson ◽  
L Prakash ◽  
S Prakash ◽  
M A Osley ◽  
S I Reed
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Steady State ◽  
Uv Light ◽  
State Level ◽  
S Phase ◽  
Dna Ligase ◽  
Mrna Levels ◽  
Steady State Level ◽  
High Level

We have cloned CDC9, the structural gene for Saccharomyces cerevisiae DNA ligase, and investigated its transcriptional regulation both as a function of cell cycle stage and after UV irradiation. The steady-state level of DNA ligase mRNA increases at least fourfold in late G1, after the completion of start but before S phase. This high level of CDC9 mRNA then decays with an apparent half-life of ca. 20 min and remains at a low basal level throughout the rest of the cell cycle. The accumulation of CDC9 mRNA in late G1 is dependent upon the completion of start but not the CDC7 and CDC8 functions. Exposure of cells to UV light elicits an eightfold increase in DNA ligase mRNA levels.

scholarly journals The steady-state level and stability of TLS polymerase eta are cell cycle dependent in the yeast S. cerevisiae

DNA Repair ◽  
2015 ◽  
Vol 29 ◽  
pp. 147-153 ◽  
Author(s):  
Michal Plachta ◽  
Agnieszka Halas ◽  
Justyna McIntyre ◽  
Ewa Sledziewska-Gojska
Keyword(s):  
Cell Cycle ◽  
Steady State ◽  
State Level ◽  
Steady State Level ◽  
Polymerase Eta ◽  
Cycle Dependent

The effect of all-trans and 9-cis retinoic acid on the steady state level of HPV16 E6/E7 mRNA and cell cycle in cervical carcinoma cells

Life Sciences ◽  
1998 ◽  
Vol 63 (7) ◽  
pp. 565-573 ◽  
Author(s):  
Bhagavathi A. Narayanan ◽  
E.Blair Holladay ◽  
Daniel W. Nixon ◽  
Charles T. Maure
Keyword(s):  
Cell Cycle ◽  
Retinoic Acid ◽  
Steady State ◽  
Cervical Carcinoma ◽  
State Level ◽  
Carcinoma Cells ◽  
Steady State Level ◽  
Hpv16 E6

Tacrolimus initial steady state level in post-transplant cyclophosphamide-based GvHD prophylaxis regimens

2021 ◽  
Author(s):  
Janny M. Yao ◽  
Dongyun Yang ◽  
Mary C. Clark ◽  
Salman Otoukesh ◽  
Thai Cao ◽  
...  
Keyword(s):  
Steady State ◽  
State Level ◽  
Steady State Level ◽  
Post Transplant ◽  
Gvhd Prophylaxis
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