Many yeast chromosomes lack the telomere-specific Y' sequence

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

scholarly journals Many yeast chromosomes lack the telomere-specific Y' sequence.

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757 ◽  
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

scholarly journals More than one variant ofListeria monocytogenesisolated from each of two human cases of invasive listeriosis

2006 ◽  
Vol 135 (5) ◽  
pp. 854-856 ◽  
Author(s):  
W. THAM ◽  
G. LOPEZ VALLADARES ◽  
S. HELMERSSON ◽  
A. ÖSTERLUND ◽  
M.-L. DANIELSSON-THAM
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Blood Cultures ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Dna Profile ◽  
Pulsed Field ◽  
Different Strains

SUMMARYTwo variants ofListeria monocytogeneswere isolated from blood cultures from each of two patients with listeriosis. Each variant displayed a two-band difference in DNA profile from the other by pulsed-field gel electrophoresis. Although this difference in profile is insufficient to distinguish clearly between the variants, the possibility of co-infection with different strains ofL. monocytogenesneeds to be considered. We suggest that more than one colony should be selected for molecular typing to aid interpretation during investigation of the sources and routes ofListeriainfection.

scholarly journals Comparison of Randomly Amplified Polymorphic DNA Analysis and Pulsed-Field Gel Electrophoresis for Typing of Moraxella catarrhalis Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 450-452 ◽  
Author(s):  
Hoang Vu-Thien ◽  
Carole Dulot ◽  
Didier Moissenet ◽  
Brigitte Fauroux ◽  
Antoine Garbarg-Chenon
Keyword(s):  
Gel Electrophoresis ◽  
Moraxella Catarrhalis ◽  
Dna Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field ◽  
Chronic Colonization ◽  
Different Strains

Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing ofM. catarrhalis.

Distribution of telomere-associated sequences on natural chromosomes in Saccharomyces cerevisiae

1988 ◽  
Vol 8 (5) ◽  
pp. 2257-2260 ◽  
Author(s):  
V A Zakian ◽  
H M Blanton
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Yeast Chromosome ◽  
Associated Sequences ◽  
Chromosome I

Pulsed-field gel electrophoresis was used to examine the distribution of telomere-associated sequences on individual chromosomes in four strains of Saccharomyces cerevisiae. The pattern of X and Y' distribution was different for each strain. At least one chromosome in each strain lacked Y', and in some strains, chromosome I, the smallest yeast chromosome, lacked detectable amounts of both X and Y'.

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