Rapd analysis of wine Saccharomyces cerevisiae strains differentiated by pulsed field gel electrophoresis

1994 ◽  
Vol 8 (8) ◽  
pp. 557-560 ◽  
Author(s):  
M. S. Grando ◽  
J. Ubeda ◽  
A. I. Briones
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gel Electrophoresis ◽  
Rapd Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Many yeast chromosomes lack the telomere-specific Y' sequence

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

scholarly journals Many yeast chromosomes lack the telomere-specific Y' sequence.

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757 ◽  
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

Distribution of telomere-associated sequences on natural chromosomes in Saccharomyces cerevisiae

1988 ◽  
Vol 8 (5) ◽  
pp. 2257-2260 ◽  
Author(s):  
V A Zakian ◽  
H M Blanton
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Yeast Chromosome ◽  
Associated Sequences ◽  
Chromosome I

Pulsed-field gel electrophoresis was used to examine the distribution of telomere-associated sequences on individual chromosomes in four strains of Saccharomyces cerevisiae. The pattern of X and Y' distribution was different for each strain. At least one chromosome in each strain lacked Y', and in some strains, chromosome I, the smallest yeast chromosome, lacked detectable amounts of both X and Y'.

scholarly journals Comparison of Ribotyping, Randomly Amplified Polymorphic DNA Analysis, and Pulsed-Field Gel Electrophoresis in Typing ofLactobacillus rhamnosus and L. caseiStrains

1999 ◽  
Vol 65 (9) ◽  
pp. 3908-3914 ◽  
Author(s):  
Soile Tynkkynen ◽  
Reetta Satokari ◽  
Maria Saarela ◽  
Tiina Mattila-Sandholm ◽  
Maija Saxelin
Keyword(s):  
Gel Electrophoresis ◽  
Type Strain ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Strain Atcc ◽  
Randomly Amplified Polymorphic Dna ◽  
Specific Primers ◽  
Pulsed Field ◽  
Species Specific

ABSTRACT A total of 24 strains, biochemically identified as members of theLactobacillus casei group, were identified by PCR with species-specific primers. The same set of strains was typed by randomly amplified polymorphic DNA (RAPD) analysis, ribotyping, and pulsed-field gel electrophoresis (PFGE) in order to compare the discriminatory power of the methods. Species-specific primers for L. rhamnosusand L. casei identified the type strain L. rhamnosus ATCC 7469 and the neotype strain L. caseiATCC 334, respectively, but did not give any signal with the recently revived species L. zeae, which contains the type strain ATCC 15820 and the strain ATCC 393, which was previously classified asL. casei. Our results are in accordance with the suggested new classification of the L. casei group. Altogether, 21 of the 24 strains studied were identified with the species-specific primers. In strain typing, PFGE was the most discriminatory method, revealing 17 genotypes for the 24 strains studied. Ribotyping and RAPD analysis yielded 15 and 12 genotypes, respectively.

scholarly journals Pulsed-Field Gel Electrophoresis Is More Efficient than Ribotyping and Random Amplified Polymorphic DNA Analysis in Discrimination of Pasteurella haemolytica Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 380-385 ◽  
Author(s):  
Angeli Kodjo ◽  
Laurence Villard ◽  
Chantal Bizet ◽  
Jean-Louis Martel ◽  
Richard Sanchis ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Pasteurella Haemolytica ◽  
Pulsed Field ◽  
Rapd Pattern ◽  
Almost All

One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA′ to HT′, that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1′ to Rp 15′) unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.

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