Identification of Enterococcus faecalis strains by DNA hybridization and pulsed-field gel electrophoresis.

1995 ◽  
Vol 33 (12) ◽  
pp. 3368-3369 ◽  
Author(s):  
T M Coque ◽  
B E Murray
Keyword(s):  
Enterococcus Faecalis ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Draft Genome Sequences of the Probiotic Enterococcus faecalis Symbioflor 1 Clones DSM16430 and DSM16434

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Moritz Fritzenwanker ◽  
Anindita Chakraborty ◽  
Torsten Hain ◽  
Kurt Zimmermann ◽  
Eugen Domann
Keyword(s):  
Comparative Analysis ◽  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Draft Genome ◽  
Pulsed Field Gel Electrophoresis ◽  
Genome Sequences ◽  
Pulsed Field

The probiotic Symbioflor 1 is a historical concoction of 10 isolates of Enterococcus faecalis . Pulsed-field gel electrophoresis revealed two groups: one comprising eight identical clones (DSM16430, DSM16432, DSM16433, DSM16435 to DSM16439) and a further two isolates (DSM16431, DSM16434) with marginally different profiles. Here, we report a comparative analysis of the draft genome sequences of representative isolates.

scholarly journals Comparison of Multilocus Variable-Number Tandem-Repeat Analysis with Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis for Enterococcus faecalis

2011 ◽  
Vol 60 (4) ◽  
pp. 335-339 ◽  
Author(s):  
EWA SADOWY ◽  
ALEKSANDRA SIEŃKO ◽  
WALERIA HRYNIEWICZ
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Tandem Repeats ◽  
Diversity Index ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Pulsed Field Gel Electrophoresis ◽  
Discriminatory Power ◽  
Pulsed Field ◽  
High Discriminatory Power

Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus sequence types and 67 pulsed-field gel electrophoresis (PFGE) profiles. MLVA showed high discriminatory power, discerning 111 different types (diversity index equal 98.9%). The concordance MLVA/MLST and MLVA/PFGE was 0.95 and 0.74, respectively. High discriminatory power of MLVA indicates its utility for local epidemiology such as outbreak investigation, and for differentiation of clones defined by other methods.

scholarly journals Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal

2004 ◽  
Vol 48 (9) ◽  
pp. 3613-3617 ◽  
Author(s):  
Carla Novais ◽  
Teresa M. Coque ◽  
João Carlos Sousa ◽  
Fernando Baquero ◽  
Luisa Peixe
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Genetic Patterns ◽  
Single Clone ◽  
Vancomycin Resistant

ABSTRACT Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

Identification of Enterococcus faecalis in Different Clonal Lineages with the Pulsed-Field Gel Electrophoresis Method in Imam Khomeini Hospital, Ilam, Iran

2021 ◽  
Vol 14 (11) ◽  
Author(s):  
Farzad Mohamadi ◽  
Jalil Vand Yousefi ◽  
Naser Harzandi ◽  
Sobhan Ghafourian
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Disk Diffusion ◽  
Pulsed Field ◽  
Vana Gene ◽  
Electrophoresis Method ◽  
Typing Methods ◽  
Vancomycin Resistant ◽  
Tract Infections

Background: Due to the importance of identifying the source of infectious agents, different typing methods have been developed, among which the pulsed-field gel electrophoresis (PFGE) method is known as the gold standard for bacteria. Also, Enterococcus faecalis is classified as a nosocomial infection. Objectives: The current study aimed to identify the source of E. faecalis by the PFGE method. Methods: Bacteria were collected from all cases of urinary tract infections. Then, the identification process was performed. All isolates were evaluated for vancomycin resistance, and then PFGE was carried out. Results: The results of disk diffusion showed that 54% of the isolates showed resistance to vancomycin. Also, 4% of the isolates were intermediate, and 42% showed sensitivity to vancomycin. Afterwards, the PCR of the VanA gene was performed to confirm the results of disk diffusion. Thus, 48 out of 54 (88.8%) isolates had the VanA gene, and none of the four intermediate isolates had the VanA gene. Our results demonstrated that 54 isolates were vancomycin-resistant, and 50 different pulsotypes groups were identified. Conclusions: Our findings showed the isolates of E. faecalis were from different clonal lineages.

Many yeast chromosomes lack the telomere-specific Y' sequence

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

scholarly journals Many yeast chromosomes lack the telomere-specific Y' sequence.

1989 ◽  
Vol 9 (12) ◽  
pp. 5754-5757 ◽  
Author(s):  
D Jäger ◽  
P Philippsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

scholarly journals Isolation of VanA-Type Vancomycin-Resistant Enterococcus Strains from Domestic Poultry Products with Enrichment by Incubation in Buffered Peptone Water at 42�C

2010 ◽  
Vol 76 (15) ◽  
pp. 5317-5320 ◽  
Author(s):  
Tetsuya Harada ◽  
Masashi Kanki ◽  
Takao Kawai ◽  
Masumi Taguchi ◽  
Tsutomu Asao ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Poultry Products ◽  
Domestic Poultry ◽  
Peptone Water ◽  
Vancomycin Resistant

ABSTRACT Eight VanA-type enterococcal strains were isolated from 8 of 171 domestic poultry products by using enrichment by incubation in buffered peptone water at 35�C and 42�C. The pulsed-field gel electrophoresis patterns of all six VanA-type Enterococcus faecalis isolates were nearly indistinguishable, indicating the presence of a specific clone in Japan.

scholarly journals Comparison of the beta-lactamase gene cluster in clonally distinct strains of Enterococcus faecalis.

1996 ◽  
Vol 40 (5) ◽  
pp. 1170-1174 ◽  
Author(s):  
J F Tomayko ◽  
K K Zscheck ◽  
K V Singh ◽  
B E Murray
Keyword(s):  
Gene Cluster ◽  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Considerable Change ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Analysis ◽  
Beta Lactamase ◽  
Pulsed Field ◽  
Large Clone ◽  
Lactamase Gene

Ten beta-lactamase-producing Enterococcus faecalis isolates were examined for the presence of the staphylococcal beta-lactamase repressor and antirepressor genes. Four isolates, previously shown to be unrelated to each other by pulsed-field gel electrophoresis analysis, were positive for both genes by PCR, although beta-lactamase production was not induced with methicillin. Six isolates, previously shown to be clonally related, were negative for both genes by PCR. The blaZ sequences of eight beta-lactamase-producing E. faecalis isolates were determined. Seven isolates from five distinct clones had sequences identical to that previously reported for E. faecalis HH22, regardless of whether the repressor or antirepressor was demonstrated by PCR. However, blaZ from one isolate differed from those of the other enterococci by 11 nucleotides; this isolate is part of the large clone, as defined by pulsed-field gel electrophoresis and multilocus enzyme analysis, that includes HH22. These findings suggest either that enterococci have acquired the bla gene cluster from more than one source or that the gene cluster has undergone considerable change since acquisition by this clone.

scholarly journals Apparent Increased Prevalence of High-Level Aminoglycoside-Resistant Enterococcus durans Resulting from False Identification by a Semiautomated Software System

1998 ◽  
Vol 36 (5) ◽  
pp. 1419-1421 ◽  
Author(s):  
Athanassios Tsakris ◽  
Neil Woodford ◽  
Spyros Pournaras ◽  
Mary Kaufmann ◽  
John Douboyas
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
University Hospital ◽  
Software System ◽  
False Identification ◽  
Enterococcus Durans ◽  
Pulsed Field ◽  
High Level

Identification of enterococci by using a semiautomated system (PASCO; Difco Laboratories, Detroit, Mich.) in the AHEPA University Hospital, Thessaloniki, Greece, revealed a high proportion ofEnterococcus durans, particularly among isolates highly resistant to gentamicin and streptomycin. When 14 isolates were further tested by a conventional biochemical scheme and by PCR, all were reidentified as Enterococcus faecalis. Antibiotic resistance and pulsed-field gel electrophoresis patterns showed that unrelated strains were misidentified.

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