scholarly journals First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

2018 ◽  
Vol 7 (23) ◽  
Author(s):  
Mustafa Ababneh ◽  
Helena L. Ferreira ◽  
Mohammad Khalifeh ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Reverse Transcriptase ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Illumina Sequencing ◽  
Newcastle Disease ◽  
Rt Pcr ◽  
Total Rna ◽  
Reverse Transcriptase Pcr ◽  
Chicken Spleen

Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing.

scholarly journals Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse Transcriptase Polymerase Chain Reaction

2017 ◽  
Vol 35 (1) ◽  
pp. 127
Author(s):  
Lehgarubini Shanmuganathan ◽  
Dito Anggoro ◽  
Michael Haryadi Wibowo
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Rna Extraction ◽  
Rt Pcr ◽  
Chain Reaction ◽  
F Gene ◽  
Polymerase Chain

Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens. Diagnosis of ND which generally involves virus isolation and subsequent identification with serological assays has limitations that needs more time. This research was aimed to detect Newcastle Disease virus (NDV) in chickens suspected with ND using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) technique. Nine chicken organ samples such as lien, trachea, and lungs were collected from chicken farms diagnosed with ND. The organ samples were processed and the targeted viral RNA was extracted using the RNA extraction kit. Genome amplification was performed with RT-PCR using specificprimers to target the F gene. Amplification results produced an amplicon product of 565 base pairs (bp). PCR product samples were then visualised using agar gel electrophoresis and viewed using the unified gel documentation system. Amplification results show nine samples positive for the DNA bands corresponding to the targeted band of the NDV F gene fragment. The results of this research confirm that the RT-PCR method is applicable for NDV detection from chicken organ samples.

scholarly journals Complete Genome Sequence of a Newcastle Disease Virus Isolated from a Rock Dove (Columba livia) in the Russian Federation

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Kseniya S. Yurchenko ◽  
Mariya V. Sivay ◽  
Alexandra V. Glushchenko ◽  
Sergey V. Alkhovsky ◽  
Alexey M. Shchetinin ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Russian Federation ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Columba Livia ◽  
The Russian Federation

scholarly journals Complete Genome Sequence of a Velogenic Newcastle Disease Virus Strain Isolated from a Clinically Healthy Exotic Parakeet (Melopsittacus undulatus) in Pakistan

2017 ◽  
Vol 5 (6) ◽  
Author(s):  
Abdul Wajid ◽  
Asma Basharat ◽  
Taseer Ahmed Khan ◽  
Muhammad Wasim ◽  
Shafqat Fatima Rehmani
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virulent Strain ◽  
Northern Region ◽  
Melopsittacus Undulatus ◽  
Virulent Newcastle Disease Virus

ABSTRACT The complete genome sequence of a virulent Newcastle disease virus (vNDV) strain isolated from an exotic parakeet (Melopsittacus undulatus) is described here. The virulent strain parakeet/Pak/R-Pindi/SFR-16/2016 was isolated from a bird reared as a pet in the province of Punjab in the northern region of Pakistan in 2016. Phylogenetic analysis classified the isolate as a member of NDV class II, subgenotype VIIi, in genotype VII.

Evaluation of RT-PCR for Rapid Detection of Sudanese Isolates and Vaccine Strains of Newcastle Disease Virus

2005 ◽  
Vol 8 (3) ◽  
pp. 418-420
Author(s):  
Mohamed A.M. Yousof . ◽  
I.E. Aradaib . ◽  
K.M.S. Khairalla . ◽  
M.A. Abdalla . ◽  
A.R.E. Karrar . ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Rapid Detection ◽  
Rt Pcr

Characterization of complete genome sequence of genotype VI and VII velogenic Newcastle disease virus from Japan

Virus Genes ◽  
2014 ◽  
Vol 49 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Dennis V. Umali ◽  
Hiroshi Ito ◽  
Kazutoshi Shirota ◽  
Hiromitsu Katoh ◽  
Toshihiro Ito
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Velogenic Newcastle Disease Virus

scholarly journals Complete Genome Sequence of a Newcastle Disease Genotype XIII Virus Isolated from Indigenous Chickens in Zambia

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Celia Abolnik ◽  
Chrisborn Mubamba ◽  
George Dautu ◽  
Bruce Gummow
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virulent Strain ◽  
African Origin ◽  
Indigenous Chickens

ABSTRACT The first complete genome sequence of an African-origin Newcastle disease virus belonging to genotype XIII is described here. The virulent strain chicken/Zambia/Chiwoko/2015 was isolated from diseased chickens in 2015.

scholarly journals Real-Time Reverse Transcription-Polymerase Chain Reaction Detection of Newcastle Disease Virus Using Light upon Extension Fluorogenic Primers

2007 ◽  
Vol 19 (4) ◽  
pp. 400-404 ◽  
Author(s):  
Márta Antal ◽  
Tibor Farkas ◽  
Péter Germán ◽  
Sándor Belák ◽  
István Kiss
Keyword(s):  
Newcastle Disease Virus ◽  
Real Time ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Plasmid Copy Number ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Plasmid Copy ◽  
Infectious Dose ◽  
Efficient Detection

A real-time reverse transcriptase (RT)-PCR assay, applying light upon extension (LUX) fluorogenic primers, was developed for rapid and efficient detection of Newcastle disease virus (NDV). The method, which targets the fusion (F) protein gene of the viral genome, gave positive signal with all NDV isolates tested (32/32), while negative results were obtained with heterologous pathogens (35/35), including 13 avian influenza virus isolates. The detection limit of the assay was approximately 10+1.2 egg infectious dose (EID)50/0.2 ml and 10+2.2 EID50/0.2 ml for virus suspensions and spiked chicken fecal samples, respectively. As expressed in plasmid copy number, the procedure has a sensitivity of approximately 20 copies of the plasmid harboring the target gene. Due to its high specificity, sensitivity, and relative simplicity, the LUX RT-PCR assay provides a novel, rapid, and practical tool for the detection of NDV.

scholarly journals Complete Genome Sequence of a Genotype XVII Newcastle Disease Virus, Isolated from an Apparently Healthy Domestic Duck in Nigeria

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ismaila Shittu ◽  
Poonam Sharma ◽  
Tony M. Joannis ◽  
Jeremy D. Volkening ◽  
Georgina N. Odaibo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Protein Gene ◽  
Domestic Duck ◽  
Apparently Healthy

The first complete genome sequence of a strain of Newcastle disease virus (NDV) of genotype XVII is described here. A velogenic strain (duck/Nigeria/903/KUDU-113/1992) was isolated from an apparently healthy free-roaming domestic duck sampled in Kuru, Nigeria, in 1992. Phylogenetic analysis of the fusion protein gene and complete genome classified the isolate as a member of NDV class II, genotype XVII.

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