scholarly journals Using Cartesian Doubt To Build a Sequencing-Based View of Microbiology

mSystems ◽  
2021 ◽  
Author(s):  
Braden T. Tierney ◽  
Erika Szymanski ◽  
James R. Henriksen ◽  
Aleksandar D. Kostic ◽  
Chirag J. Patel
Keyword(s):  
Dna Sequencing ◽  
Experimental Knowledge ◽  
Cartesian Doubt ◽  
Modern Tool

The technological leap of DNA sequencing generated a tension between modern metagenomics and historical microbiology. We are forcibly harmonizing the output of a modern tool with centuries of experimental knowledge derived from culture-based microbiology.

Imaging polymers, proteins and dna in aqueous solutions with the atomic force microscope

1989 ◽  
Vol 47 ◽  
pp. 32-33
Author(s):  
S.A.C. Gould ◽  
B. Drake ◽  
C.B. Prater ◽  
A.L. Weisenhorn ◽  
S.M. Lindsay ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Sequencing ◽  
Aqueous Solutions ◽  
Atomic Force Microscope ◽  
Piezoelectric Crystal ◽  
Atomic Force ◽  
Structure Of Molecules ◽  
Optical Lever

The atomic force microscope (AFM) is an instrument that can be used to image many samples of interest in biology and medicine. Images of polymerized amino acids, polyalanine and polyphenylalanine demonstrate the potential of the AFM for revealing the structure of molecules. Images of the protein fibrinogen which agree with TEM images demonstrate that the AFM can provide topographical data on larger molecules. Finally, images of DNA suggest the AFM may soon provide an easier and faster technique for DNA sequencing.The AFM consists of a microfabricated SiO2 triangular shaped cantilever with a diamond tip affixed at the elbow to act as a probe. The sample is mounted on a electronically driven piezoelectric crystal. It is then placed in contact with the tip and scanned. The topography of the surface causes minute deflections in the 100 μm long cantilever which are detected using an optical lever.

TB OR NOT TB?… THAT IS THE QUESTION: A RARE MYCOBACTERIUM (M. INTERMEDIUM) IDENTIFIED BY DNA SEQUENCING

2001 ◽  
Vol 28 (10) ◽  
pp. 549-554
Author(s):  
Ryan N. Cole ◽  
Stewart W. West ◽  
Christine L. Terrell ◽  
Glenn D. Roberts ◽  
Iftikhar Ahmed
Keyword(s):  
Dna Sequencing

A brief history of (DNA sequencing) time

2007 ◽  
Vol 8 (S1) ◽  
pp. S21-S21
Author(s):  
Elaine R. Mardis
Keyword(s):  
Dna Sequencing ◽  
History Of

Direct Read and Quantify Damage Nucleotide from an Oligonucleotide Using a Single Molecule Interface

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Meng-Yin Li ◽  
Jie Yang ◽  
Ya-Qian Wang ◽  
Xue-Yuan Wu ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Single Molecule ◽  
Genetic Diseases ◽  
High Sensitivity ◽  
Dna Lesions ◽  
Lesion Detection ◽  
The Novel ◽  
Structural Variations ◽  
Dna Lesion ◽  
Base Lesion

DNA lesion such as metholcytosine(<sup>m</sup>C), 8-OXO-guanine(<sup>O</sup>G), inosine(I) <i>etc</i> could cause the genetic diseases. Identification of the varieties of lesion bases are usually beyond the capability of conventional DNA sequencing which is mainly designed to discriminate four bases only. Therefore, lesion detection remain challenge due to the massive varieties and less distinguishable readouts for minor structural variations. Moreover, standard amplification and labelling hardly works in DNA lesions detection. Herein, we designed a single molecule interface from the mutant K238Q Aerolysin, whose confined sensing region shows the high compatible to capture and then directly convert each base lesion into distinguishable current readouts. Compared with previous single molecule sensing interface, the resolution of the K238Q Aerolysin nanopore is enhanced by 2-order. The novel K238Q could direct discriminate at least 3 types (<sup>m</sup>C, <sup>O</sup>G, I) lesions without lableing and quantify modification sites under mixed hetero-composition condition of oligonucleotide. Such nanopore could be further applied to diagnose genetic diseases at high sensitivity.

Lateral Adsorbate Interactions Inhibit HCOO- While Promoting CO Selectivity for CO2 Electrocatalysis on Ag

2018 ◽  
Author(s):  
Divya Bohra ◽  
Isis Ledezma-Yanez ◽  
Guanna Li ◽  
Wiebren De Jong ◽  
Evgeny A. Pidko ◽  
...  
Keyword(s):  
Electrochemical Reduction ◽  
Experimental Approach ◽  
Decisive Role ◽  
Experimental Methods ◽  
Reaction Intermediates ◽  
Intermediate Species ◽  
Experimental Knowledge ◽  
Complex Interactions ◽  
Ag Catalysts

<p>The analysis presented in this manuscript helps bridge an important fundamental discrepancy between the existing theoretical and experimental knowledge regarding the performance of Ag catalysts for CO<sub>2</sub> electrochemical reduction (CO<sub>2</sub>ER). The results demonstrate how the intermediate species *OCHO is formed readily en-route the HCOO<sup>– </sup>pathway and plays a decisive role in determining selectivity of a predominantly CO producing catalyst such as Ag. Our theoretical and experimental approach develops a better understanding of the nature of competition as well as the complex interactions between the reaction intermediates leading to CO, HCOO<sup>–</sup> and H<sub>2</sub> during CO<sub>2</sub>ER.</p><p><br></p><p>Details of computational and experimental methods are present in the Supporting Information provided. </p><p><br></p><p><br></p>

Probiotics are a modern tool for a clinician

2018 ◽  
pp. 59-62
Author(s):  
E.A. Yablokova ◽  
◽  
A.V. Gorelov ◽  
Keyword(s):  
Modern Tool

Lean Manufacturing- A Tool for Improvement of Production System

2016 ◽  
Vol 8 (02) ◽  
Author(s):  
José G. Vargas- Hernández ◽  
María Teresa Jiménez Castillo
Keyword(s):  
Research Methods ◽  
Production System ◽  
Lean Manufacturing ◽  
Relevant Information ◽  
Research Project ◽  
Success Stories ◽  
Modern Tool

The present research project aims to make known the benefits of Lean Manufacturing on a production system. The application of this modern tool of Lean Manufacturing helps us record the changes generated by its application on different companies. This is analyzed by using different research methods, such as collection and documented analysis. Finally the results are exposed through the data organised in table and graphs highlighting the efficiency of this tool by checking their validity. Success stories are also exposed in the implementation and relevant information drawn that could be used as a basic for new businesses that decide to use this application

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