Subcellular localization and detection of Tobacco mosaic virus ORF6 protein by immunoelectron microscopy

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

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Identification of maize mosaic virus by immunoelectron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142827 ◽

1986 ◽

Vol 44 ◽

pp. 240-241

Author(s):

O. E. Bradfute

Keyword(s):

Zea Mays ◽

Mosaic Virus ◽

Immunoelectron Microscopy ◽

Severe Disease ◽

Maize Mosaic Virus ◽

The World ◽

Plant Rhabdovirus

Maize mosaic virus (MMV) causes a severe disease of Zea mays in many tropical and subtropical regions of the world, including the southern U.S. (1-3). Fig. 1 shows internal cross striations of helical nucleoprotein and bounding membrane with surface projections typical of many plant rhabdovirus particles including MMV (3). Immunoelectron microscopy (IEM) was investigated as a method for identifying MMV. Antiserum to MMV was supplied by Ramon Lastra (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela).

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Tobacco Mosaic Virus-Infected Tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119375 ◽

1985 ◽

Vol 43 ◽

pp. 510-511

Author(s):

Egbert W. Henry

Keyword(s):

Nicotiana Tabacum ◽

Tobacco Mosaic Virus ◽

Chlorogenic Acid ◽

Mosaic Virus ◽

Host Resistance ◽

Oxidative Metabolism ◽

Leaf Tissue ◽

Vein Clearing ◽

Basal Septum ◽

Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

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A method for computing low-frequency normal modes of large symmetric assemblies of macromolecules : Application to the disk of tobacco mosaic virus protein

Journal de Chimie Physique ◽

10.1051/jcp/1991882726 ◽

1991 ◽

Vol 88 ◽

pp. 2726-2726

Author(s):

T Simonson ◽

D Perahia

Keyword(s):

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Normal Modes ◽

Low Frequency ◽

Virus Protein

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