An analysis of the bioluminescence intensity dynamics of the luminous bacteria Photobacterium phosphoreum

BIOPHYSICS ◽  
2015 ◽  
Vol 60 (2) ◽  
pp. 251-255 ◽  
Author(s):  
A. V. Drozdov ◽  
E. N. Gromozova ◽  
I. A. Gretsky
Keyword(s):  
Photobacterium Phosphoreum ◽  
Bioluminescence Intensity ◽  
Luminous Bacteria

scholarly journals Genomic and Phylogenetic Characterization of Luminous Bacteria Symbiotic with the Deep-Sea Fish Chlorophthalmus albatrossis (Aulopiformes: Chlorophthalmidae)

2005 ◽  
Vol 71 (2) ◽  
pp. 930-939 ◽  
Author(s):  
Paul V. Dunlap ◽  
Jennifer C. Ast
Keyword(s):  
Deep Sea ◽  
Type Strain ◽  
Photobacterium Phosphoreum ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Genomic Profiling ◽  
Light Organ ◽  
Phylogenetic Characterization ◽  
Luminous Bacteria ◽  
High Level

ABSTRACT Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate between phenetically similar but evolutionarily distinct luminous bacteria. Therefore, to test the species identification of chlorophthalmid symbionts, we carried out a genomotypic (repetitive element palindromic PCR genomic profiling) and phylogenetic analysis on strains isolated from the perirectal light organ of Chlorophthalmus albatrossis. Sequence analysis of the 16S rRNA gene of 10 strains from 5 fish specimens placed these bacteria in a cluster related to but phylogenetically distinct from the type strain of P. phosphoreum, ATCC 11040T, and the type strain of Photobacterium iliopiscarium, ATCC 51760T. Analysis of gyrB resolved the C. albatrossis strains as a strongly supported clade distinct from P. phosphoreum and P. iliopiscarium. Genomic profiling of 109 strains from the 5 C. albatrossis specimens revealed a high level of similarity among strains but allowed identification of genomotypically different types from each fish. Representatives of each type were then analyzed phylogenetically, using sequence of the luxABFE genes. As with gyrB, analysis of luxABFE resolved the C. albatrossis strains as a robustly supported clade distinct from P. phosphoreum. Furthermore, other strains of luminous bacteria reported as P. phosphoreum, i.e., NCIMB 844, from the skin of Merluccius capensis (Merlucciidae), NZ-11D, from the light organ of Nezumia aequalis (Macrouridae), and pjapo.1.1, from the light organ of Physiculus japonicus (Moridae), grouped phylogenetically by gyrB and luxABFE with the C. albatrossis strains, not with ATCC 11040T. These results demonstrate that luminous bacteria symbiotic with C. albatrossis, together with certain other strains of luminous bacteria, form a clade, designated the kishitanii clade, that is related to but evolutionarily distinct from P. phosphoreum. Members of the kishitanii clade may constitute the major or sole bioluminescent symbiont of several families of deep-sea luminous fishes.

scholarly journals Effects of Hydrostatic Pressure on Growth and Luminescence of a Moderately-Piezophilic Luminous Bacteria Photobacterium phosphoreum ANT-2200

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66580 ◽  
Author(s):  
Séverine Martini ◽  
Badr Al Ali ◽  
Marc Garel ◽  
David Nerini ◽  
Vincent Grossi ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Photobacterium Phosphoreum ◽  
Luminous Bacteria

scholarly journals Isolation and Identification of Photobacterium phosphoreum from an Unexpected Niche: Migrating Salmon

2003 ◽  
Vol 69 (11) ◽  
pp. 6938-6942 ◽  
Author(s):  
K. J. Budsberg ◽  
C. F. Wimpee ◽  
J. F. Braddock
Keyword(s):  
Marine Environment ◽  
Marine Bacterium ◽  
Photobacterium Phosphoreum ◽  
Isolation And Identification ◽  
Yukon River ◽  
Luminous Bacteria

ABSTRACT Six luminous bacteria were isolated from migrating salmon in the Yukon River, Alaska. All isolates were identified as Photobacterium phosphoreum. Previous studies suggest that P. phosphoreum is an exclusively marine bacterium, while our Alaskan isolates are from salmon which migrated up to 1,228 km from the marine environment.

scholarly journals Determinasi Bakteri Simbion Luminesensi Cumi Loligo edulis Serta Analisis Potensinya Sebagai Anti Bakteri

2017 ◽  
Vol 20 (2) ◽  
pp. 78
Author(s):  
Delianis Pringgenies ◽  
Dinny Anjang Sari ◽  
Ria Azizah T.N. ◽  
Ervia Yudiati ◽  
Endang Sri Susilo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Vibrio Harveyi ◽  
Bacterial Species ◽  
Photobacterium Phosphoreum ◽  
Light Organ ◽  
Bacillus Sp ◽  
Activity Test ◽  
Luminous Bacteria

The aim of this research is to determine luminous bacteria that symbiont with the light organ of the squid Loligo  edulis and also to find out the potency of simbiont bacteria in light organ of the squid L. edulis. The squid L. edulis were collected from Teluk Awur Waters, Jepara.  Stages of this research began with the isolation of bacteria, bacteria screening for anti bacteria, antibacterial activity test and and determination of bacterial species of the isolate. The result of determination showed that luminous bacteria which symbiont with the light organ of the squid L. edulis is come from species of  Photobacterium phosphoreum.  The result showed that luminous bacteria which symbiont with the light organ of the squid L. edulis have potency as antibacterial compound. Bacteria P. phosphoreum which symbiont in light organ of the squid L. edulis could inhibit the growth of Vibrio harveyi (diameter of resistance zone had range from 8,30-8,87 mm), Escherichia coli (diameter of resistance zone had range from 7,84-8,45 mm), Staphylococcus aureus (diameter of resistance zone had range from 8,39-9,09 mm) and Bacillus sp (diameter of resistance zone had range from 8,27-9,01).                                                                  Tujuan dari penelitian ini adalah untuk mendeterminasi bakteri simbion yang bercahaya yang pada organ ringan dan mengetahui potensi bakteri simbion pada organ ringan dari cumi cumi Loligo edulis. Cumi L. edulis dikoleksi dari perairan Teluk Awur, Jepara. Tahapan penelitian meliputi:  isolasi bakteri, skrining bakteri, uji aktivitas antibakteri dan dan identifikasi  spesies bakteri isolat. Hasil penelitian memperlihatkan bahwa bakteri luminensensi yang bersimbiosis dengan organ cahaya cumi L. edulis adalah bakteri jenis Photobacterium phosphoreum. Bakteri luminesensi yang menempel pada organ cahaya cumi L. edulis memiliki potensi sebagai senyawa antibakteri. Bakteri P. phosphoreum yang tergabung edulis dapat menghambat pertumbuhan bakteri pathogen Vibrio harveyi (diameter zona resistansi berkisar antara 8,30-8,87 mm), Escherichia coli (diameter zona resistansi berkisar antara 7,84-8,45 mm), Staphylococcus aureus (diameter zona resistansi berkisar antara 8,39-9,09 mm) dan Bacillus sp (diameter zona resistansi berkisar antara 8,27-9,01). 

Polymerase Chain reaction and 16S rRNA gene sequences from the luminous bacterial symbionts of two deep-sea anglerfishes

1992 ◽  
Vol 72 (1) ◽  
pp. 149-159 ◽  
Author(s):  
Margo G. Haygood ◽  
Daniel L. Distel ◽  
Peter J. Herring
Keyword(s):  
Polymerase Chain Reaction ◽  
Deep Sea ◽  
Direct Sequencing ◽  
Photobacterium Phosphoreum ◽  
Rrna Gene ◽  
Bacterial Symbionts ◽  
Reaction Products ◽  
Chain Reaction ◽  
Luminous Bacteria ◽  
Polymerase Chain

Sequences of the 16S ribosomal RNA gene of luminous bacterial symbionts from the escas of the deep sea anglerfishesMelanocetus johnsoniandCryptopsaras couesiwere determined by direct sequencing of polymerase chain reaction products. A sequence was also obtained from a strain ofPhotobacterium phosphoreum, the culturable light organ symbiont ofOpisthoproctus grimaldii. Comparison of these and other published sequences showed that the anglerfish symbionts group with the marine luminous bacteria but are not closely related toP. phosphoreum. The two ceratioid symbionts differ from each other at least at the species level.

scholarly journals Factors Influencing Histamine Formation by Psychrotrophic Luminous Bacteria Photobacterium phosphoreum.

1994 ◽  
Vol 60 (6) ◽  
pp. 773-777 ◽  
Author(s):  
Hideaki Morii ◽  
Yoshihiko Izumi ◽  
Kentaro Kasama ◽  
Ryo Ishimoto
Keyword(s):  
Photobacterium Phosphoreum ◽  
Factors Influencing ◽  
Histamine Formation ◽  
Luminous Bacteria

Toxicity of Para-Chloro Substituted Benzene Derivatives in the Microtox™ Test

1985 ◽  
Vol 20 (2) ◽  
pp. 36-43 ◽  
Author(s):  
Klaus L.E. Kaiser ◽  
Juan M. Ribo ◽  
Brian M. Zaruk
Keyword(s):  
General Formula ◽  
Functional Group ◽  
Structural Parameters ◽  
Systematic Investigation ◽  
Photobacterium Phosphoreum ◽  
Benzene Derivatives ◽  
Quantitative Structure ◽  
Chlorinated Benzenes ◽  
Chloro Derivatives ◽  
Derivatives Of

Abstract This paper gives the results of part of a systematic investigation into contaminant toxicity to Photobacterium phosphoreum in the Microtox™ test. Reported are the toxicity values for 39 para-chloro substituted benzene derivatives of the general formula l-Cl-C6h4-4-X=CH2CH(NH2)COOH, F, SO2NH2, OCH2COOH, CH2COOH, CONHNH2, NHCOCH3, CONH2, CH=CHCOOH, SeOOH, CH2NH2, CH2CH2NH2, NO2, H, CF3, CHO, CH2OH, OH, CH3, CCl3, COCH3, COOH, NH2, SO2C6H5, Cl, CH2COCH3, COCl, CN, OCH3, NCO, NHCH3, I, COC6H5, CH2Cl, SH, CH2SH, NCS, CH2CN and SO2C6H4Cl. Except for the last compound, whose solubility is below the required concentration, the toxicities increase in the presented order with a total range of more than three orders of magnitude. The data are discussed in terms of quantitative structure-toxicity correlations with compound-specific structural parameters. In combination with a previously developed submodel on chlorinated benzenes, phenols, nitrobenzenes and anilines, the observed relationships allow the prediction of the toxicity of some 780 possible chloro derivatives of the general formula C6H5-nClnX, where n=<5 and X is a functional group as listed above.

Sign in / Sign up

Export Citation Format

Share Document