Polymerase Chain reaction and 16S rRNA gene sequences from the luminous bacterial symbionts of two deep-sea anglerfishes

1992 ◽  
Vol 72 (1) ◽  
pp. 149-159 ◽  
Author(s):  
Margo G. Haygood ◽  
Daniel L. Distel ◽  
Peter J. Herring
Keyword(s):  
Polymerase Chain Reaction ◽  
Deep Sea ◽  
Direct Sequencing ◽  
Photobacterium Phosphoreum ◽  
Rrna Gene ◽  
Bacterial Symbionts ◽  
Reaction Products ◽  
Chain Reaction ◽  
Luminous Bacteria ◽  
Polymerase Chain

Sequences of the 16S ribosomal RNA gene of luminous bacterial symbionts from the escas of the deep sea anglerfishesMelanocetus johnsoniandCryptopsaras couesiwere determined by direct sequencing of polymerase chain reaction products. A sequence was also obtained from a strain ofPhotobacterium phosphoreum, the culturable light organ symbiont ofOpisthoproctus grimaldii. Comparison of these and other published sequences showed that the anglerfish symbionts group with the marine luminous bacteria but are not closely related toP. phosphoreum. The two ceratioid symbionts differ from each other at least at the species level.

scholarly journals Cloning and Characterization of a Self-compatible Sf Haplotype in Almond [Prunus dulcis (Mill.) D.A. Webb. syn. P. amygdalus Batsch] to Resolve Previous Confusion in Its Sf-RNase Sequence

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 609-613 ◽  
Author(s):  
Toshio Hanada ◽  
Kyoko Fukuta ◽  
Hisayo Yamane ◽  
Tomoya Esumi ◽  
Ryutaro Tao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Mechanisms ◽  
Direct Sequencing ◽  
Genomic Structure ◽  
Prunus Dulcis ◽  
Physical Distance ◽  
Histidine Residue ◽  
Reaction Products ◽  
Chain Reaction ◽  
Polymerase Chain

Most of the self-compatible (SC) cultivars of almond [Prunus dulcis (Mill.) D.A. Webb. syn. P. amygdalus Batsch] have the Sf haplotype. In this study, we cloned and characterized the S locus region of the Sf haplotype of SC ‘Lauranne’. The relative transcriptional orientation of SFBf and Sf-RNase and the physical distance between them are similar to those of other functional self-incompatible (SI) S haplotypes of Prunus, indicating that the genomic structure of the SC Sf haplotype appears to be intact. Although there is no apparent mutation in the coding sequence of SFBf, the Sf-RNase sequence in this study and previously reported Sf-RNase sequences show discrepancies. First, as opposed to previous indications, the ‘Lauranne’ Sf-RNase sequence encodes a histidine residue in place of a previously reported arginine residue in the conserved C2 region of Prunus S-RNase. Direct sequencing of the polymerase chain reaction products from the Sf-RNase of ‘Tuono’ confirmed that ‘Tuono’ Sf-RNase also encodes the histidine residue. We found another difference in the ‘Lauranne’ Sf-RNase sequence and other reported Sf-RNase sequences. Namely, ‘Lauranne’ Sf-RNase encodes a phenylalanine residue in place of a previously reported leucine residue in the conserved C5 region of Prunus S-RNase. This is also the case for ‘Tuono’ Sf-RNase. Expression analysis of Sf-RNase and SFBf by reverse transcriptase–polymerase chain reaction showed that Sf-RNase transcripts were barely detectable in pistil, whereas SFBf transcripts were accumulated at a similar level to the level that was observed with SFB of other functional SI S haplotypes of almond. We discuss the possible molecular mechanisms of SC observed with the Sf haplotype with special references to the expression of Sf-RNase.

Audiologic Features of Hearing Loss Due to the 1,555 Mutation of Mitochondrial DNA

1997 ◽  
Vol 106 (8) ◽  
pp. 643-648 ◽  
Author(s):  
Takashi Tsuiki ◽  
Kazuo Murai ◽  
Ken Kitamura ◽  
Seiko Murai ◽  
Yuya Tamagawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Hearing Loss ◽  
Mitochondrial Dna ◽  
Direct Sequencing ◽  
High Tone ◽  
Reaction Products ◽  
Chain Reaction ◽  
Cycle Sequencing ◽  
Sequencing Method ◽  
Polymerase Chain

We proved a 1,555 mutation of mitochondrial DNA in one member of each of three families with familial streptomycin hearing loss, and report the pedigrees and audiologic features. DNA was extracted by the standard method. The 1,555 A to G mutation was identified in all three patients and confirmed by direct sequencing of the polymerase chain reaction products by a cycle sequencing method. On audiograms, the hearing loss was sensorineural, bilateral, and symmetric, showing a high-tone loss or a profound loss particularly in the high-tone range, and the “symmetry law” of Langenbeck was applicable. The superimposed audiograms of members of one family did not cross themselves, proving the applicability of the “never-cross principle of audiograms.”

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