Reactive oxygen species generated in mitochondria induce fragmentation of the mitochondrial reticulum in HeLa cells

2008 ◽  
Vol 420 (1) ◽  
pp. 221-223 ◽  
Author(s):  
O. K. Nepryakhina ◽  
A. Yu. Kuznetsova ◽  
K. G. Lyamzaev ◽  
D. S. Izyumov ◽  
O. Yu. Pletjushkina ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hela Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Reticulum

GNG11 (G-protein subunit γ 11) suppresses cell growth with induction of reactive oxygen species and abnormal nuclear morphology in human SUSM-1 cells

2017 ◽  
Vol 95 (4) ◽  
pp. 517-523 ◽  
Author(s):  
Yuki Takauji ◽  
Ikuru Kudo ◽  
Atsuki En ◽  
Ryo Matsuo ◽  
Mohammad Nazir Hossain ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
G Protein ◽  
Cell Lines ◽  
Hela Cells ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Nuclear Morphology ◽  
Oxygen Species ◽  
Protein Subunit ◽  
Regulatory Relationship

Enforced expression of GNG11, G-protein subunit γ 11, induces cellular senescence in normal human diploid fibroblasts. We here examined the effect of the expression of GNG11 on the growth of immortalized human cell lines, and found that it suppressed the growth of SUSM-1 cells, but not of HeLa cells. We then compared these two cell lines to understand the molecular basis for the action of GNG11. We found that expression of GNG11 induced the generation of reactive oxygen species (ROS) and abnormal nuclear morphology in SUSM-1 cells but not in HeLa cells. Increased ROS generation by GNG11 would likely be caused by the down-regulation of the antioxidant enzymes in SUSM-1 cells. We also found that SUSM-1 cells, even under normal culture conditions, showed higher levels of ROS and higher incidence of abnormal nuclear morphology than HeLa cells, and that abnormal nuclear morphology was relevant to the increased ROS generation in SUSM-1 cells. Thus, SUSM-1 and HeLa cells showed differences in the regulation of ROS and nuclear morphology, which might account for their different responses to the expression of GNG11. Thus, SUSM-1 cells may provide a unique system to study the regulatory relationship between ROS generation, nuclear morphology, and G-protein signaling.

scholarly journals Effects of Reactive Oxygen Species on Cell Proliferation and Death in HeLa Cells and Its MDR1-Overexpressing Derivative Cell Line

2003 ◽  
Vol 26 (2) ◽  
pp. 278-281 ◽  
Author(s):  
Yu-Wen Liu ◽  
Toshiyuki Sakaeda ◽  
Kohji Takara ◽  
Tsutomu Nakamura ◽  
Nobuko Ohmoto ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Cell Line ◽  
Hela Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Antibody ligation of CM1 on cisplatin-exposed HeLa cells induces apoptosis through reactive oxygen species-dependent Fas ligand expression

2014 ◽  
Vol 44 (6) ◽  
pp. 2016-2024 ◽  
Author(s):  
GA BIN PARK ◽  
DAEJIN KIM ◽  
HOI SOO YOON ◽  
YEONG-SEOK KIM ◽  
HYUN-KYUNG LEE ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hela Cells ◽  
Fas Ligand ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ligand Expression

scholarly journals Upconversion fluorescence imaging of HeLa cells using ROS generating SiO2-coated lanthanide-doped NaYF4 nanoconstructs

RSC Advances ◽  
2017 ◽  
Vol 7 (48) ◽  
pp. 30262-30273 ◽  
Author(s):  
Przemysław Kowalik ◽  
Danek Elbaum ◽  
Jakub Mikulski ◽  
Krzysztof Fronc ◽  
Izabela Kamińska ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fluorescence Imaging ◽  
Hela Cells ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Upconversion Fluorescence ◽  
Coated Nanoparticles

Multicolor upconversion of SiO2-coated nanoparticles using for cells imaging and reactive oxygen species generation.

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