The link between Soil Methane Oxidation Rate and Abundance of Methanotrophs Estimated by Quantitative PCR

Microbiology ◽  
2020 ◽  
Vol 89 (2) ◽  
pp. 182-191
Author(s):  
A. F. Sabrekov ◽  
M. V. Semenov ◽  
I. E. Terent’eva ◽  
Yu. V. Litti ◽  
D. V. Il’yasov ◽  
...  
2016 ◽  
Vol 77 ◽  
pp. 103-107 ◽  
Author(s):  
V.Yu. Bychkov ◽  
Yu.P. Tulenin ◽  
M.M. Slinko ◽  
A.K. Khudorozhkov ◽  
V.I. Bukhtiyarov ◽  
...  

2019 ◽  
Author(s):  
Jun Murase ◽  
Atsuko Sugimoto ◽  
Ryo Shingubara ◽  
Tomoki Morozumi ◽  
Shinya Takano ◽  
...  

Abstract. Arctic wetlands are significant sources of atmospheric methane and the observed accelerated climate changes in the arctic could cause the change in methane dynamics, where methane oxidation would be the key process to control methane emission from wetlands. In this study we determined the potential methane oxidation rate of the wetland soils of a taiga-tundra transition zone in northeastern Siberia. Peat soil samples were collected in summer from depressions covered with tussocks of sedges and Sphagnum spp. and from mounds vegetated with moss and larch trees. A bottle incubation experiment demonstrated that the soil samples collected from depressions in the moss- and sedge-dominated zones exhibited active methane oxidation with no time lag. The potential methane oxidation rates at 15 °C ranged from 94 to 496 nmol h−1 g−1 dw. Methane oxidation was observed over the depths studied (0–40 cm) including the water-saturated anoxic layers. The maximum methane oxidation rate was recorded in the layer above the water-saturated layer: the surface (0–2 cm) layer in the sedge-dominated zone and in the middle (4–6 cm) layer in the moss-dominated zone. The methane oxidation rate was temperature-dependent, and the threshold temperature of methane oxidation was estimated to be −4 to −11 °C, which suggested methane oxidation at subzero temperatures. Soil samples collected from the frozen layer of Sphagnum peat also showed immediate methane consumption when incubated at 15 °C. The present results suggest that the methane oxidizing bacteria in the wetland soils keep their potential activities even under anoxic and frozen conditions and immediately utilize methane when the conditions become favorable. On the other hand, the inhibitor of methane oxidation did not affect the methane flux from the sedge and moss zones in situ, which indicated the minor role of plant-associated methane oxidation.


2021 ◽  
Author(s):  
Semra Bakkaloglu ◽  
Dave Lowry ◽  
Rebecca Fisher ◽  
James France ◽  
Euan Nisbet

<p>Biological methane oxidation in landfill cover material can be characterised using stable isotopes. Methane oxidation fraction is calculated from the carbon isotopic signature of emitted CH<sub>4</sub>, with enhanced microbial consumption of methane in the aerobic portion of the landfill cover indicated by a shift to less depleted isotopic values in the residual methane emitted to air. This study was performed at four southwest England landfill sites. Mobile mole fraction measurement at the four sites was coupled with Flexfoil bag sampling of air for high-precision isotope analysis. Gas well samples collected from the pipeline systems and downwind plume air samples were utilized to estimate methane oxidation rate for whole sites. This work was designed to assess the impact on carbon isotopic signature and oxidation rate as UK landfill practice and waste streams have changed in recent years.</p><p>The landfill status such as closed and active, seasonal variation, cap stripping and site closure impact on landfill isotopic signature and oxidation rate were evaluated. The isotopic signature of <sup>13</sup>C-CH<sub>4</sub> values of emissions varied between -60 and -54‰, with an averaged value of -57 +- 2‰ for methane from closed and active landfill sites. Methane emissions from older, closed landfill sites were typically more enriched in <sup>13</sup>C than emissions from active sites. This study found that the isotopic signature of <sup>13</sup>C-CH<sub>4</sub> of fugitive methane did not show a seasonal trend, and there was no plume observed from a partial cap stripping process to assess changes in <sup>13</sup>C-CH<sub>4</sub>  isotopic signatures of emitted methane. Also, the closure of an active landfill cell caused a significant decrease in mole fraction of measured CH<sub>4</sub>, which was less depleted <sup>13</sup>C in the emitted plume due to a higher oxidation rate. Methane oxidation, estimated from the isotope fractionation, ranged from 3 to 27%, with mean values of 7% and 15% for active and closed landfills, respectively. These results indicate that the oxidation rate is highly site specific.</p><p> </p>


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