scholarly journals Methane oxidation potential of the arctic wetland soils of a taiga-tundra ecotone in northeastern Siberia

2019 ◽  
Author(s):  
Jun Murase ◽  
Atsuko Sugimoto ◽  
Ryo Shingubara ◽  
Tomoki Morozumi ◽  
Shinya Takano ◽  
...  
Keyword(s):  
Methane Oxidation ◽  
Oxidation Rate ◽  
Time Lag ◽  
Soil Samples ◽  
The Arctic ◽  
Wetland Soils ◽  
Oxidation Rates ◽  
Northeastern Siberia ◽  
Water Saturated ◽  
Methane Oxidation Rate

Abstract. Arctic wetlands are significant sources of atmospheric methane and the observed accelerated climate changes in the arctic could cause the change in methane dynamics, where methane oxidation would be the key process to control methane emission from wetlands. In this study we determined the potential methane oxidation rate of the wetland soils of a taiga-tundra transition zone in northeastern Siberia. Peat soil samples were collected in summer from depressions covered with tussocks of sedges and Sphagnum spp. and from mounds vegetated with moss and larch trees. A bottle incubation experiment demonstrated that the soil samples collected from depressions in the moss- and sedge-dominated zones exhibited active methane oxidation with no time lag. The potential methane oxidation rates at 15 °C ranged from 94 to 496 nmol h−1 g−1 dw. Methane oxidation was observed over the depths studied (0–40 cm) including the water-saturated anoxic layers. The maximum methane oxidation rate was recorded in the layer above the water-saturated layer: the surface (0–2 cm) layer in the sedge-dominated zone and in the middle (4–6 cm) layer in the moss-dominated zone. The methane oxidation rate was temperature-dependent, and the threshold temperature of methane oxidation was estimated to be −4 to −11 °C, which suggested methane oxidation at subzero temperatures. Soil samples collected from the frozen layer of Sphagnum peat also showed immediate methane consumption when incubated at 15 °C. The present results suggest that the methane oxidizing bacteria in the wetland soils keep their potential activities even under anoxic and frozen conditions and immediately utilize methane when the conditions become favorable. On the other hand, the inhibitor of methane oxidation did not affect the methane flux from the sedge and moss zones in situ, which indicated the minor role of plant-associated methane oxidation.

Methane oxidation potential of the arctic wetland soils of a taiga-tundra ecotone in northeastern Siberia

2020 ◽  
Vol 66 (4) ◽  
pp. 645-652
Author(s):  
Jun Murase ◽  
Atsuko Sugimoto ◽  
Ryo Shingubara ◽  
Maochang Liang ◽  
Tomoki Morozumi ◽  
...  
Keyword(s):  
Methane Oxidation ◽  
Oxidation Potential ◽  
The Arctic ◽  
Wetland Soils ◽  
Northeastern Siberia

UK landfill methane emissions: Use of mobile plume measurements and carbon isotopic characterisation to reassess oxidation rates for open and closed sites 

2021 ◽  
Author(s):  
Semra Bakkaloglu ◽  
Dave Lowry ◽  
Rebecca Fisher ◽  
James France ◽  
Euan Nisbet
Keyword(s):  
Methane Oxidation ◽  
Mole Fraction ◽  
Oxidation Rate ◽  
Methane Emissions ◽  
Isotopic Signature ◽  
Oxidation Rates ◽  
Landfill Cover ◽  
Carbon Isotopic ◽  
Landfill Sites ◽  
The Impact

<p>Biological methane oxidation in landfill cover material can be characterised using stable isotopes. Methane oxidation fraction is calculated from the carbon isotopic signature of emitted CH<sub>4</sub>, with enhanced microbial consumption of methane in the aerobic portion of the landfill cover indicated by a shift to less depleted isotopic values in the residual methane emitted to air. This study was performed at four southwest England landfill sites. Mobile mole fraction measurement at the four sites was coupled with Flexfoil bag sampling of air for high-precision isotope analysis. Gas well samples collected from the pipeline systems and downwind plume air samples were utilized to estimate methane oxidation rate for whole sites. This work was designed to assess the impact on carbon isotopic signature and oxidation rate as UK landfill practice and waste streams have changed in recent years.</p><p>The landfill status such as closed and active, seasonal variation, cap stripping and site closure impact on landfill isotopic signature and oxidation rate were evaluated. The isotopic signature of <sup>13</sup>C-CH<sub>4</sub> values of emissions varied between -60 and -54‰, with an averaged value of -57 +- 2‰ for methane from closed and active landfill sites. Methane emissions from older, closed landfill sites were typically more enriched in <sup>13</sup>C than emissions from active sites. This study found that the isotopic signature of <sup>13</sup>C-CH<sub>4</sub> of fugitive methane did not show a seasonal trend, and there was no plume observed from a partial cap stripping process to assess changes in <sup>13</sup>C-CH<sub>4</sub>  isotopic signatures of emitted methane. Also, the closure of an active landfill cell caused a significant decrease in mole fraction of measured CH<sub>4</sub>, which was less depleted <sup>13</sup>C in the emitted plume due to a higher oxidation rate. Methane oxidation, estimated from the isotope fractionation, ranged from 3 to 27%, with mean values of 7% and 15% for active and closed landfills, respectively. These results indicate that the oxidation rate is highly site specific.</p><p> </p>

The link between Soil Methane Oxidation Rate and Abundance of Methanotrophs Estimated by Quantitative PCR

Microbiology ◽  
2020 ◽  
Vol 89 (2) ◽  
pp. 182-191
Author(s):  
A. F. Sabrekov ◽  
M. V. Semenov ◽  
I. E. Terent’eva ◽  
Yu. V. Litti ◽  
D. V. Il’yasov ◽  
...  
Keyword(s):  
Methane Oxidation ◽  
Quantitative Pcr ◽  
Oxidation Rate ◽  
Methane Oxidation Rate ◽  
Soil Methane Oxidation

Self-oscillations of methane oxidation rate over Pd/Al2O3 catalysts: Role of Pd particle size

2016 ◽  
Vol 77 ◽  
pp. 103-107 ◽  
Author(s):  
V.Yu. Bychkov ◽  
Yu.P. Tulenin ◽  
M.M. Slinko ◽  
A.K. Khudorozhkov ◽  
V.I. Bukhtiyarov ◽  
...  
Keyword(s):  
Particle Size ◽  
Methane Oxidation ◽  
Oxidation Rate ◽  
Methane Oxidation Rate

scholarly journals Evidence for the Cooccurrence of Nitrite-Dependent Anaerobic Ammonium and Methane Oxidation Processes in a Flooded Paddy Field

2014 ◽  
Vol 80 (24) ◽  
pp. 7611-7619 ◽  
Author(s):  
Li-dong Shen ◽  
Shuai Liu ◽  
Qian Huang ◽  
Xu Lian ◽  
Zhan-fei He ◽  
...  
Keyword(s):  
Methane Oxidation ◽  
Paddy Field ◽  
Dry Weight ◽  
Anammox Bacteria ◽  
Wetland Soils ◽  
Soil Cores ◽  
Ammonium Oxidation ◽  
Content Type ◽  
Oxidation Rates ◽  
Damo Bacteria

ABSTRACTAnaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) are two of the most recent discoveries in the microbial nitrogen cycle. In the present study, we provide direct evidence for the cooccurrence of the anammox and n-damo processes in a flooded paddy field in southeastern China. Stable isotope experiments showed that the potential anammox rates ranged from 5.6 to 22.7 nmol N2g−1(dry weight) day−1and the potential n-damo rates varied from 0.2 to 2.1 nmol CO2g−1(dry weight) day−1in different layers of soil cores. Quantitative PCR showed that the abundance of anammox bacteria ranged from 1.0 × 105to 2.0 × 106copies g−1(dry weight) in different layers of soil cores and the abundance of n-damo bacteria varied from 3.8 × 105to 6.1 × 106copies g−1(dry weight). Phylogenetic analyses of the recovered 16S rRNA gene sequences showed that anammox bacteria affiliated with “CandidatusBrocadia” and “CandidatusKuenenia” and n-damo bacteria related to “CandidatusMethylomirabilis oxyfera” were present in the soil cores. It is estimated that a total loss of 50.7 g N m−2per year could be linked to the anammox process, which is at intermediate levels for the nitrogen flux ranges of aerobic ammonium oxidation and denitrification reported in wetland soils. In addition, it is estimated that a total of 0.14 g CH4m−2per year could be oxidized via the n-damo process, while this rate is at the lower end of the aerobic methane oxidation rates reported in wetland soils.

scholarly journals Methanotrophic activity and bacterial diversity in volcanic-geothermal soils at Pantelleria island (Italy)

2014 ◽  
Vol 11 (4) ◽  
pp. 5147-5178
Author(s):  
A. L. Gagliano ◽  
W. D'Alessandro ◽  
M. Tagliavia ◽  
F. Parello ◽  
P. Quatrini
Keyword(s):  
Bacterial Diversity ◽  
Methane Oxidation ◽  
Active Site ◽  
Oxidation Rate ◽  
Soil Samples ◽  
Geothermal Systems ◽  
Soil Dna ◽  
Methane Consumption ◽  
Top Soil ◽  
Geothermal Soils

Abstract. Volcanic and geothermal systems emit endogenous gases by widespread degassing from soils, including CH4, a greenhouse gas twenty-five times as potent as CO2. Recently, it has been demonstrated that volcanic/geothermal soils are source of methane, but also sites of methanotrophic activity. Methanotrophs are able to consume 10–40 Tg of CH4 a−1 and to trap more than 50% of the methane degassing through the soils. We report on methane microbial oxidation in the geothermally most active site of Pantelleria island (Italy), Favara Grande, whose total methane emission was previously estimated in about 2.5 t a−1. Laboratory incubation experiments with three top-soil samples from Favara Grande indicated methane consumption values up to 950 ng g−1 dry soil h−1. One of the three sites, FAV2, where the highest oxidation rate was detected, was further analysed on a vertical soil profile and the maximum methane consumption was measured in the top-soil layer but values > 100 ng g−1 h−1 were maintained up to a depth of 15 cm. The highest consumption rate was measured at 37 °C, but a still recognizable consumption at 80 °C (> 20 ng g−1 h−1) was recorded. In order to estimate the bacterial diversity, total soil DNA was extracted from Favara Grande and analysed using a Temporal Temperature Gradient gel Electrophoresis (TTGE) analysis of the amplified bacterial 16S rRNA gene. The three soil samples were probed by PCR using standard proteobacterial primers and newly designed verrucomicrobial primers targeting the unique methane monooxygenase gene pmoA; the presence of methanotrophs was detected in sites FAV2 and FAV3, but not in FAV1, where harsher chemical-physical conditions and negligible methane oxidation were detected. The pmoA gene libraries from the most active site FAV2 pointed out a high diversity of gammaproteobacterial methanotrophs distantly related to Methylococcus/Methylothermus genera and the presence of the newly discovered acido-thermophilic methanotrophs Verrucomicrobia. Alphaproteobacteria of the genus Methylocystis were isolated from enrichment cultures, under a methane containing atmosphere at 37 °C. The isolates grow at pH 3.5–8 and temperatures of 18–45 °C, and show a methane oxidation rate of ~ 450 μmol mol−1 h−1. Soils from Favara Grande showed the largest diversity of methanotrophic bacteria until now detected in a geothermal soil. While methanotrophic Verrucomicrobia are reported to dominate highly acidic geothermal sites, our results suggest that slightly acidic soils, in high enthalpy geothermal systems, host a more diverse group of both culturable and uncultivated methanotrophs.

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