The genetic diversity of burbot (Lota lota L., 1758) of Western Siberia (the analysis of the mtDNA control region polymorphism)

<p>Notolabrus celidotus (the New Zealand spotty) is a common rocky reef species that is endemic to New Zealand. This species is the most abundant demersal reef fish in New Zealand, and is distributed throughout the North and South Islands, and Stewart Island. Notolabrus celidotus consumes a wide variety of small invertebrates, and juveniles are reliant on coastal kelp forests as nursery habitats. Because N. celidotus is such a common species on New Zealand rocky reefs it is a good model species for population genetic studies. The primary goal of this research was to investigate new genetic markers and add new sample locations to bolster previous genetic population data from N. celidotus. The thesis research utilised DNA sequences obtained from a 454 massively parallel DNA sequencer and reports six new microsatellite loci for N. celidotus. These loci are the first microsatellite DNA markers to be developed for this species. Additional mitochondrial DNA (mtDNA) control region sequences were obtained from new samples of N. celidotus and combined with previously reported mtDNA sequences. Increasing the sample size improved the genetic coverage of N. celidotus populations around coastal New Zealand. The mtDNA sequences were analysed to examine the population connectivity and demographic history of N. celidotus. The microsatellite DNA loci reported in this study were also used to examine the levels of genetic diversity and population structure in N. celidotus. Results of the combined genetic analyses revealed extremely high levels of genetic diversity among the population sample of the mtDNA control region. Both the mitochondrial DNA and microsatellite DNA analyses showed a distinct lack of population genetic structuring, which suggests there is constant mixing of N. celidotus among sites. The results of this study have the potential to inform the expectations about the genetic structure of closely related wrasse species, such as Notolabrus fucicola, as well as other coastal species that have a similar life history, dispersal power, and New Zealand-wide distribution.</p>

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New data on the phylogeography and genetic diversity of the brown bear Ursus arctos Linnaeus, 1758 of Northeastern Eurasia (mtDNA control region polymorphism analysis)

Biology Bulletin ◽

10.1134/s1062359014010087 ◽

2014 ◽

Vol 41 (1) ◽

pp. 38-46 ◽

Cited By ~ 6

Author(s):

V. V. Salomashkina ◽

M. V. Kholodova ◽

O. Yu. Tuten’kov ◽

N. S. Moskvitina ◽

N. G. Erokhin

Keyword(s):

Genetic Diversity ◽

Control Region ◽

Ursus Arctos ◽

Brown Bear ◽

Mtdna Control Region ◽

Polymorphism Analysis

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Ex situ conservation status of an endangered Yangtze finless porpoise population (Neophocaena phocaenoides asiaeorientalis) as measured from microsatellites and mtDNA diversity

ICES Journal of Marine Science ◽

10.1016/j.icesjms.2005.06.002 ◽

2005 ◽

Vol 62 (8) ◽

pp. 1711-1716 ◽

Cited By ~ 12

Author(s):

Junhong Xia ◽

Jinsong Zheng ◽

Ding Wang

Keyword(s):

Genetic Diversity ◽

Control Region ◽

Ex Situ Conservation ◽

Ex Situ ◽

Mtdna Control Region ◽

Finless Porpoise ◽

Yangtze Finless Porpoise ◽

Control Region Sequences ◽

Neophocaena Phocaenoides ◽

Neophocaena Phocaenoides Asiaeorientalis

Abstract The Yangtze finless porpoise, Neophocaena phocaenoides asiaeorientalis, is an endangered small cetacean that occurs only in the middle and lower reaches of the Yangtze River of China. The establishment of a breeding population of the porpoise in Tian-e-Zhou Baiji National Natural Reserve represents the first attempt at ex situ conservation efforts for a cetacean species. With the goal of effective protection, management, and monitoring of this preserved population, we examined its genetic diversity using 930 bp of mtDNA control region sequences and 13 polymorphic microsatellite loci. A very low level of genetic variation (h = 0.6010 ± 0.0029 s.d.; π = 0.0007 ± 0.0000002 s.d.) in the mtDNA control region sequences and a moderate genetic diversity (Ho = 0.5740 ± 0.2575 s.d.) in the microsatellites were detected in the population. It is necessary to introduce more individuals with representative genetic variations into the reserve in order to form a larger and healthier group structure for long-term survival of the population. Successful establishment of the Yangtze finless porpoise population in the Reserve also provides a useful model for an ex situ conservation programme for other rare and endangered species.

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Low genetic diversity in the Endangered Crested Ibis Nipponia nippon and implications for conservation

Bird Conservation International ◽

10.1017/s0959270904000231 ◽

2004 ◽

Vol 14 (3) ◽

pp. 183-190 ◽

Cited By ~ 22

Author(s):

BEI ZHANG ◽

SHENG-GUO FANG ◽

YONG-MEI XI

Keyword(s):

Genetic Diversity ◽

Control Region ◽

Haplotype Diversity ◽

Mtdna Control Region ◽

Significant Information ◽

Nipponia Nippon ◽

Low Genetic Diversity ◽

Captive Populations ◽

Crested Ibis ◽

Wide Range

Crested Ibis Nipponia nippon is an Endangered species that has experienced a severe but short-lived population bottleneck in recent years. Only one wild population and two captive populations exist in China today, all of which were probably initiated from two breeding pairs rediscovered in 1981. This paper represents the first study of the genetic diversity of Crested Ibis by analysing sequence variation of a fragment spanning domains II and III of the mitochondrial DNA (mtDNA) control region of both wild and captive populations. Two haplotypes were identified in both wild and captive populations, and the captive population had a bias towards haplotype 1. The species as a whole demonstrated an extremely low level of genetic diversity, with a haplotype diversity and nucleotide diversity of 0.386 ± 0.074 and 0.069% ± 0.013%, respectively. Despite the rapid increase in numbers of birds under intensive management, the paucity of genetic diversity remains a real threat to the species. The mtDNA control region variations detected in the present study could provide significant information additional to the studbook data of Crested Ibis; therefore we suggest that special attention be paid to individuals with haplotype 2 when considering captive breeding management. Overall, great care should be taken in the selection of reintroduction sites for this rare bird, as species with low genetic diversity are thought to be more limited in their ability to tolerate a wide range of environmental extremes and diseases.

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