Reverse phase HPLC determination of sunitinib malate using UV detector, its isomerisation study, method development and validation

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of methanol: water (85:15), with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 10-110µg/ml with a regression equation y=88048x + 44524 and having a regression value (R2) of 0.999. The LOD and LOQ values found to be 10ng and 100ng, respectively. No changes found in ruggedness and robustness studies. The percentage of recovery was found to be 95.25% to 101.81%. Validation studies revealed that the method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis in its pharmaceutical formulations.

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A multivariate quantification of Box‐Behnken design assisted method development and validation of dextromethorphan hydrobromide and desloratadine simultaneously by reverse‐phase HPLC in in‐house syrup formulation

Journal of Separation Science ◽

10.1002/jssc.202000510 ◽

2020 ◽

Vol 43 (18) ◽

pp. 3597-3606

Author(s):

Md. Irshad Alam ◽

Aquil ur‐Rahim Siddiqui ◽

Nazia Khanam ◽

Shaikh Jalil Kamaruddin

Keyword(s):

Method Development ◽

Reverse Phase Hplc ◽

Reverse Phase ◽

Box Behnken Design ◽

Method Development And Validation ◽

Development And Validation ◽

Dextromethorphan Hydrobromide

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METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF PIOGLITAZONE IN BULK SAMPLES AS WELL AS IN TABLET DOSAGE FORMS BY USING RP -HPL C

INDIAN DRUGS ◽

10.53879/id.53.09.10394 ◽

2016 ◽

Vol 53 (09) ◽

pp. 42-46

Author(s):

M Debnath ◽

◽

A. S. Kumar ◽

V. D. S Ganta

Keyword(s):

Method Development ◽

Dosage Forms ◽

Hplc Method ◽

Uv Detector ◽

Pharmaceutical Dosage Forms ◽

Method Development And Validation ◽

The Mean ◽

Development And Validation ◽

Tablet Dosage

A simple and precise RP‐HPLC method was developed and validated for the determination of pioglitazone hydrochloride in pharmaceutical dosage forms. Chromatography was carried out using Kromosil- C18 ODS column (250 x 4.6 mm; 5 μm), mixture of acetate buffer: methanol (40:60 v/v) as the mobile phase at a flow rate 1.0 mL/min. The analyte was monitored using UV detector at 254 nm. The retention time for pioglitazone HCl was 3.063 min. The proposed method was found linear in the concentration range of 20.0‐70.0 μg/ml with correlation coefficient of r2=0.9999. The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for pioglitazone HCl were in the range 99.20-101.59%. Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining pioglitazone HCl in bulk and dosage forms.

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Bioanalytical method development and validation of UV spectrophotometric method for estimation of Bumetanide spiked in human urine

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.11.2.0298 ◽

2021 ◽

Vol 11 (2) ◽

pp. 241-248

Author(s):

Bhavya sri Khagga ◽

Kavya.Parelli

Keyword(s):

Spectrophotometric Method ◽

Human Urine ◽

Method Development ◽

Uv Detector ◽

Bioanalytical Method ◽

Method Development And Validation ◽

Uv Visible ◽

Development And Validation ◽

Spiked Human Urine

The main purpose of this study was to develop a simple precise, rapid and accurate UV-visible spectrophotometric method for determination of Bumetanide in spiked human urine by extracting the Bumetanide from spiked human urine using ethyl acetate after extraction it was scanned between 200-400nm by using UV detector and its absorbance maxima was found to be 222nm.The calibration curve was linear in the range of 1-17 µg/ml. .the recovery and assay studies of bumetanide were within 93-94.85% indicating that the proposed method can be estimation of bumetanide.

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METHOD DEVELOPMENT AND VALIDATION OF EPROSARTAN MESYLATE AND ITS IMPURITIES USING REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2016v8i4.15277 ◽

2016 ◽

Vol 8 (4) ◽

pp. 49 ◽

Cited By ~ 1

Author(s):

O. S. S. Chandana ◽

D. Sathis Kumar ◽

R. Ravichandra Babu

Keyword(s):

High Performance ◽

Method Development ◽

Hplc Method ◽

Reverse Phase ◽

Related Substances ◽

Rp Hplc ◽

Method Development And Validation ◽

The Mean ◽

Development And Validation

Objective: Our main objective is to develop an accurate and precise RP-HPLC method for the determination of Eprosartan Mesylate and its impurities. Methods: A Develosil ODS UG-5; (150 × 4.6) mm; 5 µm column was used for the Separation of drugs by a mobile phase consisting of Buffer and Acetonitrile mixture in the gradient proportion. The flow rate maintained was 0.8 ml/min and the wavelength used for detection was 235 nm.Results: The linearity was observed in the range of 0.025-50µg/ml of spiked impurities in Eprosartan Mesylate, impurity 1 and impurity 2 with a correlation coefficient of 0.99927, 0.99910 and 0.99934 respectively. The mean percentage recoveries for LOQ, 50%, 80%, 100%, 150% and 200% accuracy were found to be 101.5±1.51, 107.0±1.7, 104.6±0.4, 102.8±0.36, 101.7±0.26 and 101.3±0.15 respectively for impurities in Eprosartan Mesylate, impurity 1 and impurity 2. Linearity, accuracy, precision and robustness parameters for the suggested method were estimated for validation.Conclusion: The developed method is uncomplicated, accurate, sensitive and precise for the determination of related substances in the Eprosartan Mesylate. The satisfying % recoveries and low % RSD Values confirmed the suitability of the developed method for the usual analysis of Eprosartan mesylate in pharmaceuticals.

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