The method of noncovalent in vitro binding of target proteins to virus-like nanoparticles formed by core antigen of hepatitis B virus

2013 ◽  
Vol 448 (1) ◽  
pp. 52-54 ◽  
Author(s):  
E. A. Blokhina ◽  
V. V. Kupriyanov ◽  
N. V. Ravin ◽  
K. G. Skryabin
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Antigen ◽  
Target Proteins ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
B Virus

Transcription of human hepatitis B virus core antigen gene sequences in an in vitro HeLa cellular extract

Virology ◽  
1981 ◽  
Vol 111 (2) ◽  
pp. 647-652 ◽  
Author(s):  
Prasanta R. Chakraborty ◽  
Nelson Ruiz-Opazo ◽  
David A. Shafritz
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Antigen ◽  
Gene Sequences ◽  
Antigen Gene ◽  
Virus Core ◽  
Human Hepatitis ◽  
B Virus ◽  
Cellular Extract

Hepatitis B virus encodes an RNA polymerase III transcript

1983 ◽  
Vol 3 (10) ◽  
pp. 1774-1782
Author(s):  
D N Standring ◽  
L B Rall ◽  
O Laub ◽  
W J Rutter
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Rna Polymerase ◽  
Rna Polymerase Iii ◽  
Core Antigen ◽  
Transcription System ◽  
Polymerase Iii ◽  
B Virus ◽  
Sequence Elements

We demonstrated that cloned hepatitis B virus (HBV) DNA directs the synthesis of a 700-base RNA (HBV 700) by RNA polymerase III in a cell-free transcription system. HBV 700 is the only transcript known to originate from the viral short strand and has been mapped to the region between roughly 1,635 and 954 base pairs on the viral map, between the surface and core antigen coding sequences but overlapping and opposing the putative DNA polymerase and B protein genes. The in vitro initiation sites for the HBV 700 and core antigen RNAs are only 50 bases apart, suggesting that these two genes may be coordinately regulated. Moreover, both of these initiation sites appear to lie within the approximately 300-base double-stranded region (the nick region) between the 5' end of the HBV short strand and the nick in the viral long strand. We found two unusual sequence elements in the nick region that are conserved between the human and woodchuck viruses.

scholarly journals Hepatitis B virus encodes an RNA polymerase III transcript.

1983 ◽  
Vol 3 (10) ◽  
pp. 1774-1782 ◽  
Author(s):  
D N Standring ◽  
L B Rall ◽  
O Laub ◽  
W J Rutter
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Rna Polymerase ◽  
Rna Polymerase Iii ◽  
Core Antigen ◽  
Transcription System ◽  
Polymerase Iii ◽  
B Virus ◽  
Sequence Elements

We demonstrated that cloned hepatitis B virus (HBV) DNA directs the synthesis of a 700-base RNA (HBV 700) by RNA polymerase III in a cell-free transcription system. HBV 700 is the only transcript known to originate from the viral short strand and has been mapped to the region between roughly 1,635 and 954 base pairs on the viral map, between the surface and core antigen coding sequences but overlapping and opposing the putative DNA polymerase and B protein genes. The in vitro initiation sites for the HBV 700 and core antigen RNAs are only 50 bases apart, suggesting that these two genes may be coordinately regulated. Moreover, both of these initiation sites appear to lie within the approximately 300-base double-stranded region (the nick region) between the 5' end of the HBV short strand and the nick in the viral long strand. We found two unusual sequence elements in the nick region that are conserved between the human and woodchuck viruses.

scholarly journals In Vivo Modelling of Hepatitis B Virus Subgenotype A1 Replication Using Adeno-Associated Viral Vectors

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2247
Author(s):  
Shonisani Wendy Limani ◽  
Njabulo Mnyandu ◽  
Abdullah Ely ◽  
Reubina Wadee ◽  
Anna Kramvis ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Vectors ◽  
Cultured Cells ◽  
Sub Saharan Africa ◽  
Interferon Response ◽  
Core Antigen ◽  
B Virus

The paucity of animal models that simulate the replication of the hepatitis B virus (HBV) is an impediment to advancing new anti-viral treatments. The work reported here employed recombinant adeno-associated viruses (AAVs) to model HBV subgenotype A1 and subgenotype D3 replication in vitro and in vivo. Infection with subgenotype A1 is endemic to parts of sub-Saharan Africa, and it is associated with a high risk of hepatocellular carcinoma. Recombinant AAV serotype 2 (AAV2) and 8 (AAV8) vectors bearing greater-than-genome-length sequences of HBV DNA from subgenotype A1 and D3, were produced. Transduced liver-derived cultured cells produced HBV surface antigen and core antigen. Administration of AAV8 carrying HBV subgenotype A1 genome (AAV8-A1) to mice resulted in the sustained production of HBV replication markers over a six-month period, without elevated inflammatory cytokines, expression of interferon response genes or alanine transaminase activity. Markers of replication were generally higher in animals treated with subgenotype D3 genome-bearing AAVs than in those receiving the subgenotype A1-genome-bearing vectors. To validate the use of the AAV8-A1 murine model for anti-HBV drug development, the efficacy of anti-HBV artificial primary-microRNAs was assessed. Significant silencing of HBV markers was observed over a 6-month period after administering AAVs. These data indicate that AAVs conveniently and safely recapitulate the replication of different HBV subgenotypes, and the vectors may be used to assess antivirals’ potency.

scholarly journals Evaluation of the Seroprevalence of Infectious Diseases in 2,445 in vitro Fertilization Cycles

2021 ◽  
Vol 43 (03) ◽  
pp. 216-219
Author(s):  
João Guilherme Grassi dos Anjos ◽  
Newton Sergio de Carvalho ◽  
Karam Abou Saab ◽  
Edward Araujo ◽  
Jaime Kulak
Keyword(s):  
Hepatitis B Virus ◽  
In Vitro Fertilization ◽  
Hepatitis B ◽  
Core Antigen ◽  
Virus Surface ◽  
Vitro Fertilization ◽  
Human T Cell ◽  
B Virus ◽  
Hepatitis B Core Antigen

Abstract Objective To evaluate the seroprevalence of positive markers for syphilis, human immunodeficiency virus (HIV) I and II, human T cell lymphotropic virus (HTLV) I and II, and hepatitis B and C among women undergoing in vitro fertilization (IVF). Methods We conducted a retrospective analysis among patients who underwent IVF, between January 2013 and February 2016, and who had complete screening records. Results We analyzed 1,008 patients who underwent IVF, amounting to 2,445 cycles. Two patients (0.2%) tested positive for HIV I and II and none for HTLV I and II. Three patients (0.3%) had positive screening for syphilis, and two (0.2%) had positive hepatitis C antibody test (anti-HCV). A positive hepatitis B virus surface antigen (HbsAg) test was observed in 4 patients (0.4%), while 47 (4.7%) patients were positive for IgG antibody to hepatitis B core antigen (anti-HbC IgG), and only 1 (0.1%) was positive for IgM antibody to hepatitis B core antigen (anti-HbC IgM). The anti-HbS test was negative in 659 patients (65.3%). Only 34.7% of the patients had immunity against the Hepatitis B virus. Patients with an anti-HbS negative result were older than those with a hepatitis B test (anti-HbS) positive result (36.3 versus 34.9; p < 0.001). Conclusion The present study showed lower infection rates than the Brazilian ones for the diseases studied in patients undergoing IVF. Only a few patients were immunized against hepatitis B.

Sign in / Sign up

Export Citation Format

Share Document